Nebulized N-Acetyl Cysteine Protects the Pulmonary Graft Inside the Non–Heart-Beating Donor

Rega, Filip; Wuyts, Wim; Vanaudenaerde, Bart M.; Jannis, Nicole; Neyrinck, Arne; Verleden, Geert M.; Lerut, Toni; Raemdonck, Dirk Van

doi:10.1016/j.healun.2004.10.013

Cited by 32 publications

(38 citation statements)

References 35 publications

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“…In pigs undergoing isolated ventilation and reperfusion, NAC aerosol administration immediately before and after EVLP prevented warm ischemic damage compared to animals without treatment (75).…”

Section: N-acetyl-cysteine (Nac)mentioning

confidence: 90%

Oxidative and nitrosative stress during pulmonary ischemia-reperfusion injury: from the lab to the OR

Gielis¹,

Beckers²,

Briedé³

et al. 2017

Ann. Transl. Med.

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Oxidative and nitrosative stress are an umbrella term for pathophysiological processes that involve free radical generation during inflammation. In this review, the involvement of reactive oxygen and nitrogen species is evaluated during lung ischemia-reperfusion injury (LIRI) from a surgical point of view. The main biochemical and cellular mechanisms behind free radical generation are discussed, together with surgical procedures that may cause reperfusion injury. Finally, different therapeutic strategies are further explored. A literature search was performed, searching for "lung ischemia reperfusion injury", "reperfusion injury", "large animal model" and different search terms for each section: "surgery", "treatment", "cellular mechanism", or "enzyme". Although reperfusion injury is not an uncommon entity and there is a lot of evidence concerning myocardial ischemia-reperfusion injury, in the lung this phenomenon is less extensively described and studies in large animals are not easy to come by. With increasing number of patients on waiting lists for lung transplant, awareness for this entity should all but rise.

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Section: N-acetyl-cysteine (Nac)mentioning

confidence: 90%

Oxidative and nitrosative stress during pulmonary ischemia-reperfusion injury: from the lab to the OR

Gielis¹,

Beckers²,

Briedé³

et al. 2017

Ann. Transl. Med.

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“…Thus its offer directly into the bloodstream are loosely based on reports suggesting that this dose and time prior to application, are suitable for tissue concentrations sufficient to minimize or eliminate the effects of biochemical and structural repercussions on the parenchyma pulmonar [27][28][29] .…”

Section: Discussionmentioning

confidence: 99%

The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Castro

Lauz

et al. 2012

Acta Cir. Bras.

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PURPOSE:To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS:The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed. Key words: Acetylcysteine. Lung. Reperfusion. Ischemia. Liver. Rabbits. RESUMO OBJETIVO:Estudar as lesões no fígado e no pulmão de coelhos, provocadas pela isquemia/reperfusão hepática (I/R) moduladas pelo uso da N-acetil-cisteína (NAC). MÉTODOS: Vinte e quatro coelhos distribuídos em dois grupos: Grupo controle GI (n=12) solução de glicose 5% e Grupo experimento GII (n=12) NAC. Os animais foram pré-anestesiados com maleato de acepromazina 1% e anestesiados com cloridrato de quetamina 10% e xilazina 2% via intramuscular. Os grupos GI e GII receberam solução glicosada ou NAC respectivamente via endovenosa 15min antes da oclusão do pedículo hepático (30 min). Após iniciou-se o período de reperfusão por 24h (n=6) ou 48h (n=6), terminada a reperfusão, amostras do fígado e pulmão foram coletadas para a histologia e imunoistoquímica para avaliar o comprometimento celular. RESULTADOS: Os animais do grupo GII-24h e GII-48h apresentaram arquitetura do parênquima hepático próximo do normal, quando se utilizou NAC. Os grupos GII-24h e GII-48h apresentaram menor espessura das células alveolares que os grupos GI-24h e GI-48h. A expressão da caspase 3 nas células pulmonares do grupo GII, apresentou valor menor comparada ao grupo GI. CONCLUSÃO: A N-acetil-cisteína administrada 15min prévios a lesão da isquemia/reperfusão teve um papel protetor significativo minimizando as lesões morfológicas e apoptóticas pulmonares nos períodos observados. Descritores: Acetilcisteína. Pulmão. Reperfusão. Isquemia. Fígado. Coelhos.

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“…The group from Leuven have shown good experimental results in terms of lung function using antiinfl ammatory drugs nebulized after arrest. 34,35 Another point of controversy is the method of evaluating and the acceptability of criteria for these special grafts. With uncontrolled NHBDs, there is no way to investigate the quality of the lungs in terms of oxygenation in the same manner as for brain-death donors.…”

Section: Discussionmentioning

confidence: 99%

Non-heart-beating donation in Spain

Gomez-de-Antonio

Varela

2011

Gen Thorac Cardiovasc Surg

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Nebulized N-Acetyl Cysteine Protects the Pulmonary Graft Inside the Non–Heart-Beating Donor

Cited by 32 publications

References 35 publications

Oxidative and nitrosative stress during pulmonary ischemia-reperfusion injury: from the lab to the OR

Oxidative and nitrosative stress during pulmonary ischemia-reperfusion injury: from the lab to the OR

The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Non-heart-beating donation in Spain

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