Necropsy of the mouse

Rasmussen, Lorna; McInnes, Elizabeth F.

doi:10.1002/9781118789568.ch1

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Necropsy Procedures. Complete necropsies were conducted on sacrificed mice, according to standardized protocols 35,36 , and age-associated lesions were recorded. Before sacrificing, whole body weight measurements were documented, and gross examinations were conducted on the extracted thymus and spleen after trimming peri-thymic and peri-splenic adipose tissue, respectively.…”

Section: Methods Animal Subjectsmentioning

confidence: 99%

Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

Lagou,

Argyris,

Vodopyanov

et al. 2024

Preprint

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The thymus, a central primary lymphoid organ of the immune system, plays a key role in T cell development. Surprisingly, the thymus is quite neglected with regards to standardized pathology approaches and practices for assessing structure and function. Most studies use multispectral flow cytometry to define the dynamic composition of the thymus at the cell population level, but they are limited by lack of contextual insight. This knowledge gap hinders our understanding of various thymic conditions and pathologies, particularly how they affect thymic architecture, and subsequently, immune competence. Here, we introduce a digital pathology pipeline to address these challenges. Our approach can be coupled to analytical algorithms and utilizes rationalized morphometric assessments of thymic tissue, ranging from tissue-wide down to microanatomical and ultrastructural levels. This pipeline enables the quantitative assessment of putative changes and adaptations of thymic structure to stimuli, offering valuable insights into the pathophysiology of thymic disorders. This versatile pipeline can be applied to a wide range of conditions that may directly or indirectly affect thymic structure, ranging from various cytotoxic stimuli inducing acute thymic involution to autoimmune diseases, such as myasthenia gravis. Here, we demonstrate applicability of the method in a mouse model of age-dependent thymic involution, both by confirming established knowledge, and by providing novel insights on intrathymic remodeling in the aged thymus. Our orthogonal pipeline, with its high versatility and depth of analysis, promises to be a valuable and practical toolset for both basic and translational immunology laboratories investigating thymic function and disease.

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Section: Methods Animal Subjectsmentioning

confidence: 99%

Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

Lagou,

Argyris,

Vodopyanov

et al. 2024

Preprint

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“…Throughout the feeding period, we employ the clinical symptoms to detect the physical signs and survival rate, the mental status was tested with open field test (OFT) method and daily body weights were recorded. After 30 days of feeding, mice were processed with the cervical dislocation method, and the mice were necropsied with reference to the Lorna ’s method 49 . The jejunum and jejunal contents of the mice in the Jcon and Jm groups were taken immediately into sterile 1.0 mL lyophilized tubes and stored at − 80 °C for microbiological studies.…”

Section: Methodsmentioning

confidence: 99%

Music-based interventions in the feeding environment on the gut microbiota of mice

Niu

Zeng

et al. 2023

Sci Rep

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Gut microbiota is established to be associated with the diversity of gastrointestinal conditions, but information on the variation associated with music and gut microbes is limited. Current study revealed the impacts of music intervention during feeding on the growth performance and gut microbes of mice by using clinical symptoms and 16S rRNA sequencing techniques. The results showed that feeding mice with music had a significant increase in body weight after the 25th day. The Firmicutes and Proteobacteria were the most dominant phylum in the gut microbiota. Also, the relative abundance of the dominant bacteria was variable after musical intervention. In contrast to the control group, a significant decrease in alpha diversity analysis of gut bacterial microorganisms and Metastats analysis showed a significant increase in the relative abundance of 5 genera and one phylum after the music intervention. Moreover, the musical intervention during feeding caused modifications in the gut microbial composition of mice, as evidenced by an increase in the level of Firmicutes and Lactobacillus, while decreases the richness of pathogenic bacteria, e.g. Proteobacteria, Cyanobacteria and Muribaculaceae, etc. In summary, music intervention increased body weight and enhanced the abundance of beneficial bacteria by reducing the prevalence of pathogenic bacteria in gut microbiota of mice.

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“…The person performing the dissection at necropsy needs to be appropriately trained and skilled to ensure that the correct tissues are sampled and that minimum artifact is introduced due to poor handling. Numerous methods for dissection of the mouse have been published on the Internet and in journals (Fiette and Slaoui, ; Covelli et al., ; Rasmussen and McIness, ) and for methods for specific techniques (e.g., Swiss roll preparation for intestines; Moolenbeek and Ruitenberg, ). There is no one best method, but the dissector needs to be familiar and comfortable with a systematic approach to the dissection they are required to perform and any recordings they should make (see below section on recording data).…”

Section: Acquiring High‐quality Pathology Datamentioning

confidence: 99%

Acquiring, Recording, and Analyzing Pathology Data from Experimental Mice: An Overview

Scudamore

2014

CP Mouse Biology

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Pathology is often underutilized as an end point in mouse studies in academic research because of a lack of experience and expertise. The use of traditional pathology techniques including necropsy and microscopic analysis can be useful in identifying the basic processes underlying a phenotype and facilitating comparison with equivalent human diseases. This overview aims to provide a guide and reference to the acquisition, recording, and analysis of high-quality pathology data from experimental mice in an academic research setting.

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Necropsy of the mouse

Cited by 4 publications

References 40 publications

Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

Music-based interventions in the feeding environment on the gut microbiota of mice

Acquiring, Recording, and Analyzing Pathology Data from Experimental Mice: An Overview

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