Necrotrophic fungus Macrophomina phaseolina tolerates chromium stress through regulating antioxidant enzymes and genes expression (MSN1 and MT)

Shoaib, Amna; Nisar, Zahra; Nafisa,; Javaid, Arshad; Khurshid, Saba; Javed, Sidrah

doi:10.1007/s11356-019-04457-y

Cited by 17 publications

(8 citation statements)

References 50 publications

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“…Numerous studies have shown that Pseudomonas significantly alleviated Cr 6+ toxicity and enhanced the growth and nutrient uptake of plant as bionic bacteria [44]. Several studies have reported that extracellular reduction (75% removal rate) and adsorption by cell wall (24% removal rate) were the main physiological mechanisms of Cr(VI) removal [45,46]. However, the metabolic mechanism and cellular response have not been explored in Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

Pang

Zhang

et al. 2022

PLoS ONE

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Over exploitation of mineral resources has increasingly caused serious heavy metal contamination such as chromium (Cr). Cr(VI), the pathogenicity factor, is one of common environmental contaminants and widely known health hazards to living organisms. Therefore, it is urgent to control the polluted soil. Up to now, little is known about the regulatory mechanisms of Cr response in Pseudomonas sp. Cr13. In this study, transcriptome and differentially expressed genes in Pseudomonas sp. Cr13 strain was characterized by a comparison between Cr(VI)-treated sample and control sample using transcriptome sequencing approach. In total, 2974 genes were annotated, including 1245 (1154 down-regulated genes and 91 up-regulated genes) differentially expressed genes (DEGs). All DEGs could be assigned to 29 pathways, of which pathways related to amino acid metabolism, carbohydrate metabolism, energy metabolism and signal transduction mechanism were significantly enriched in Pseudomonas sp. Cr13. A possible mechanism for Cr toxicity response might be an active efflux which utilized a heavy metal translocating P-type ATPase to lower the intracellular Cr concentration. The down-regulated genes related to the antioxidant defense system had a key role in Cr reduction, such as SodA, Gst, osmC, BtuE, KatE, csdA and AhpC. The proteins that were visibly up-regulated, were likely to involve in alleviating Cr(VI) stress, and the significantly down-regulated genes such as MarR, Lrp, FhlA, GntR, HrcA, LysR family genes, were likely to reduce Cr(VI) induced oxidative stress. In addition, real-time quantitative PCR was used to analyze the expression patterns of some Cr responsive genes. This study reported the first identification of Cr responsive genes, and inferred the underlying regulatory mechanisms of response to Cr(VI) stress in Pseudomonas sp. Cr13.

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Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

Pang

Zhang

et al. 2022

PLoS ONE

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“…Shoaib et al . 2019). In a future study, quantifying gene regulation during exposure to Li or Co could further test the hypothesis that favourable conditions for mutation were present in pre‐exposed cultures of A. niger .…”

Section: Discussionmentioning

confidence: 99%

Tolerance of three fungal species to lithium and cobalt: Implications for bioleaching of spent rechargeable Li‐ion batteries

et al. 2021

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Aims: This paper aims to quantify the growth and organic acid production of Aspergillus niger, Penicillium chrysogenum and Penicillium simplicissimum when these fungi are exposed to varying levels of lithium (Li) and cobalt (Co). The study also tests whether pre-exposing the fungi to these metals enables the fungi to develop tolerance to Li or Co. Methods and Results: When cultures of A. niger, P. chrysogenum or P. simplicissimum were exposed to 250 mg l -1 of Li or Co, biomass production and excretion of organic acids were significantly inhibited after 5 days of growth compared to cultures grown in the absence of these metals. Pre-exposing cultures of A. niger to 250 mg l -1 of Li or Co for 20 days significantly increased biomass production when the fungus was subsequently sub-cultured into 250 or 500 mg l -1 of Li or Co. However, pre-exposure of P. chrysogenum or P. simplicissimum to 250 mg l -1 of Li or Co for 20 days did not increase biomass production. Conclusions: Aspergillus niger, but not the Penicillium species, developed tolerance to Li and to Co during the 20-day pre-exposure period. Therefore, processes that utilize fungal bioleaching with A. niger to mobilize and recover valuable metals such as Li or Co should consider a pre-exposure step for fungi to improve their tolerance to metal toxicity. Significance and Impact of the Study: Fungi may have the ability to extract valuable metals such as Li and Co from spent rechargeable batteries. However, the toxicity of the extracted metals can inhibit fungal growth and organic acid production. Pre-exposure to metals may alleviate toxicity for some fungal species. This knowledge can be used to improve the design of bioleaching protocols, increasing the potential for fungal bioleaching to become an economical and environmentally friendly method of recovering Li and Co from spent batteries.

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“…Table 2 shows that the values for the peaks corresponding to the hydroxyl groups, amides I and amides II, have not undergone significant changes or modifications. This may be because in both cases these groups are involved in the reduction of Cr (VI) and later convert to acid in the case of hydroxyl groups, in the case of amides I and II, and it is suggested that the reduction could be due to the presence of proteins [23,[56][57][58][59]. Of all cell fractions analyzed, it was observed that the fraction with the greatest capacity to biotransform toxic chromium [Cr (VI)] to a non-toxic form [Cr (III)] was the mixed membrane fractions.…”

Section: Effect Of Cr (Vi) On Ir Region Of the Subcellular Fractionmentioning

confidence: 99%

Biotransformation of Chromium (VI) via a Reductant Activity from the Fungal Strain Purpureocillium lilacinum

González

Rodríguez

Terán-Figueroa

et al. 2021

JoF

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Industrial effluents from chromium-based products lead to chromium pollution in the environment. Several technologies have been employed for the removal of chromium (Cr) from the environment, including adsorption, ion-exchange, bioremediation, etc. In this study, we isolated a Cr (VI)-resistant fungus, Purpureocillium lilacinum, from contaminated soil, which could reduce chromium. We also characterized a reductant activity of dichromate found in the cellular fraction of the fungus: optimal pH and temperature, effect of enzymatic inhibitors and enhancers, metal ions, use of electron donors, and initial Cr (VI) and protein concentration. This study also shows possible mechanisms that could be involved in the elimination of this metal. We observed an increase in the reduction of Cr (VI) activity in the presence of NADH followed by that of formate and acetate, as electron donor. This reduction was highly inhibited by EDTA followed by NaN3 and KCN, and this activity showed the highest activity at an optimal pH of 7.0 at 37 °C with a protein concentration of 3.62 µg/mL.

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Necrotrophic fungus Macrophomina phaseolina tolerates chromium stress through regulating antioxidant enzymes and genes expression (MSN1 and MT)

Cited by 17 publications

References 50 publications

Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

Tolerance of three fungal species to lithium and cobalt: Implications for bioleaching of spent rechargeable Li‐ion batteries

Biotransformation of Chromium (VI) via a Reductant Activity from the Fungal Strain Purpureocillium lilacinum

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