Nectandra cuspidata fraction and the isolated polyphenols protect fibroblasts and hairless mice skin from UVB-induced inflammation and oxidative stress

Ferreira, Lilian dos Anjos Oliveira; Melo, Cristina de Paula Barros de; Saito, Priscila; Iwanaga, Camila Cristina; Nakamura, Celso Vataru; Casagrande, Rúbia; Truiti, Maria da Conceição Torrado

doi:10.1016/j.jphotobiol.2020.111824

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…Additionally, emulsion containing EAF from Nectandra cuspidata, containing vitexin, protected hairless mice from UVB-induced oxidative stress. 30 In accordance with these findings, the protective effects of EE and EAF against UVB and UVA radiations may be linked to the presence of these flavonoids. Vitexin and isoquercetin have previously been identified in the genus Senegalia.…”

Section: Discussionsupporting

confidence: 58%

“…Wang et al 27 reported a great scavenging potential of isoquercetin (DPPH assay IC 50 7.63 µg/mL and ORAC assay 15.35 mmol TE/g), and Kang et al 28 reported an ORAC value of 14.8 mmol TE/g for vitexin. The photochemoprotective effects of vitexin was demonstrated by Kim et al 29 and Ferreira et al 30 presenting ROS scavenging activity and decrease of lipid peroxidation in UVBirradiated fibroblasts. Additionally, emulsion containing EAF from Nectandra cuspidata, containing vitexin, protected hairless mice from UVB-induced oxidative stress.…”

Section: Discussionmentioning

confidence: 92%

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Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts

Daré¹,

Ferreira²,

Ribeiro³

et al. 2021

FRA

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Objectives: Unprotected exposure to ultraviolet radiation causes oxidative damages to skin cells. Topical administration of antioxidants is a feasible strategy to prevent oxidative alterations. Therefore, the present study evaluated the photodamage attenuating potential of plant materials from Senegalia polyphylla leaves, due to previous studies relating Senegalia species as a source of antioxidant phenolic compounds. Materials and Methods: The ethanolic extract (EE) and its ethyl acetate (EAF), hydromethanolic and hexane fractions were evaluated for their total phenolic content and antioxidant capacity. The photochemoprotective effects of plant materials with higher antioxidant potential were assessed in L929 fibroblasts against ultraviolet-B (UVB) and Ultraviolet-A (UVA) radiations. Phytochemical investigation of bioactive plant material was performed and compounds identified by nuclear magnetic resonance analysis. Results: The EE and EAF presented the highest total phenolic content and antioxidant capacity, showing ferric reducing power and ability to scavenge free radicals DPPH•, ABTS• + , O 2 • − and ROO•. The EE and EAF treatments prior to UVB and UVA irradiation prevented the decrease in cell viability, and attenuated reactive oxygen species generation, reduced glutathione depletion, lipid peroxidation and plasma membrane disruption, especially with EAF treatment. Vitexin and isoquercetin, known antioxidant compounds, were isolated from EAF, which may be correlated with its photochemoprotective ability. Conclusion: Findings indicate the potential of polyphenol-enriched botanical materials, such as EAF from S. polyphylla, in preventing UVB and UVA-induced oxidative damages, due to its effective antioxidant activity and ability to attenuate redox imbalance and reduce cell damages.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 92%

Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts

Daré¹,

Ferreira²,

Ribeiro³

et al. 2021

FRA

Self Cite

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“…A set of studies analyzed the potential utilization of quercetin and luteolin in the management of several illnesses, including chronic urticaria [ 207 ]. The authors highlighted other research that emphasized the antioxidant and anti-inflammatory properties of polyphenols, as well as their potential to reduce oxidative stress and mast cell activation in the skin [ 208 , 209 ]. Furthermore, the scientists suggested that the amalgamation of polyphenols and antihistamines could potentially augment the outcomes for individuals suffering from chronic urticaria [ 210 ].…”

Section: Polyphenols and Their Significance In Skin Disease Therapymentioning

confidence: 99%

The Promising Role of Polyphenols in Skin Disorders

Farhan

2024

Molecules

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The biochemical characteristics of polyphenols contribute to their numerous advantageous impacts on human health. The existing research suggests that plant phenolics, whether consumed orally or applied directly to the skin, can be beneficial in alleviating symptoms and avoiding the development of many skin disorders. Phenolic compounds, which are both harmless and naturally present, exhibit significant potential in terms of counteracting the effects of skin damage, aging, diseases, wounds, and burns. Moreover, polyphenols play a preventive role and possess the ability to delay the progression of several skin disorders, ranging from small and discomforting to severe and potentially life-threatening ones. This article provides a concise overview of recent research on the potential therapeutic application of polyphenols for skin conditions. It specifically highlights studies that have investigated clinical trials and the use of polyphenol-based nanoformulations for the treatment of different skin ailments.

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“…3,4 Among them, oxidative stress and acute inflammation are the primary factors of skin tissue damage. 5 Glutathione (GSH) is the most abundant nonprotein thiol resistant to oxidative stress, but is depleted during inflammation. 6 In addition, GSH is a key factor in determining the redox signal, which has the functions of detoxification, regulation of cell proliferation and apoptosis, and immune function improvement.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have shown that the mechanism of UVB‐induced skin damage is a complex process that involves oxidative damage, inflammation, DNA damage, etc 3,4 . Among them, oxidative stress and acute inflammation are the primary factors of skin tissue damage 5 …”

Section: Introductionmentioning

confidence: 99%

Protective effect of γ‐glutamylcysteine against UVB radiation in NIH‐3T3 cells

Lü

Liu

Zhang

et al. 2022

Photoderm Photoimm Photomed

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Background: Ultraviolet (UV) radiation-induced oxidative stress is the main cause of photodamage to the skin. Glutathione (GSH) serves important physiological functions, including scavenging oxygen-free radicals and maintaining intracellular redox balance. γ-glutamylcysteine (γ-GC), as an immediate precursor of GSH and harboring antioxidant and anti-inflammatory properties, represents an unexplored option for skin photodamage treatment. Purpose:The purpose of this study was to investigate whether γ-GC can reduce UVBinduced NIH-3T3 cell damage. Methods:The experimental groups were as follows: control, UVB radiation, UVB radiation after pretreatment with γ-GC. Cell counting kit-8 (CCK-8) assays were used to measure cell proliferation, flow cytometry, and immunoblotting to detect the apoptosis rate and apoptosis-associated proteins. The levels of Reactive Oxygen Species (ROS), Superoxide Dismutase (SOD), and GSH/GSSG (oxidized GSH) were measured to assess oxidative stress. Immunoblotting and immunofluorescence were used to detect DNA damage. The members of the MAPK signaling pathways were detected by immunoblotting.Results: UVB irradiation significantly reduced cell viability and destroyed the oxidative defense system. Pretreatment with γ-GC reduced UVB-induced cytotoxicity, restored the oxidation defense system, and inhibited activation of the MAPK pathway.It also reduced the apoptosis rate, downregulated the levels of cleaved caspase 3 and cleaved PARP. Furthermore, pretreatment with γ-GC reduced the accumulation of γH2AX after UVB radiation exposure, indicating that γ-GC could protect cells from DNA damage. Conclusion:γ-GC protected NIH-3T3 from damage caused by UVB irradiation. The photoprotective effect of γ-GC is mediated via strengthening the endogenous antioxidant defense system, which prevents DNA damage and inhibits the activation of the MAPK pathway.

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Nectandra cuspidata fraction and the isolated polyphenols protect fibroblasts and hairless mice skin from UVB-induced inflammation and oxidative stress

Cited by 20 publications

References 39 publications

Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts

Photochemoprotective Effects of Ethyl Acetate Fraction from Senegalia polyphylla Leaves in Ultraviolet-Irradiated L929 Fibroblasts

The Promising Role of Polyphenols in Skin Disorders

Protective effect of γ‐glutamylcysteine against UVB radiation in NIH‐3T3 cells

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