2002
DOI: 10.1128/jvi.76.9.4625-4633.2002
|View full text |Cite
|
Sign up to set email alerts
|

Nef-Mediated Downregulation of CD4 Enhances Human Immunodeficiency Virus Type 1 Replication in Primary T Lymphocytes

Abstract: The accessory protein Nef plays a crucial role in primate lentivirus pathogenesis. Nef enhances human immunodeficiency virus type 1 (HIV-1) infectivity in culture and stimulates viral replication in primary T cells. In this study, we investigated the relationship between HIV-1 replication efficiency in CD4 ؉ T cells purified from human blood and two various known activities of Nef, CD4 downregulation and single-cycle infectivity enhancement. Using a battery of reporter viruses containing point mutations in nef… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

23
141
0
1

Year Published

2004
2004
2018
2018

Publication Types

Select...
4
2
1

Relationship

1
6

Authors

Journals

citations
Cited by 135 publications
(165 citation statements)
references
References 71 publications
23
141
0
1
Order By: Relevance
“…Absence of Nef expression was confirmed by immunoblot analysis of viral lysates. R8-based viral clones expressing the Nef mutant proteins T80A, LL165AA, and DD175AA were constructed as described previously (43). Coreceptor usage of all cloned viruses was verified by differential infection of P4/CCR5 Ϫ and P4/ CCR5 ϩ indicator cells (see below).…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…Absence of Nef expression was confirmed by immunoblot analysis of viral lysates. R8-based viral clones expressing the Nef mutant proteins T80A, LL165AA, and DD175AA were constructed as described previously (43). Coreceptor usage of all cloned viruses was verified by differential infection of P4/CCR5 Ϫ and P4/ CCR5 ϩ indicator cells (see below).…”
Section: Methodsmentioning
confidence: 99%
“…HIV-1 replication was analyzed as previously described (43). Briefly, 2 ng of each 293T-produced virus was inoculated onto 80,000 activated T cells in IL-2-containing medium (200 l).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…The interaction of Nef with p6* may be of a similar nature on the molecular level. Since Nef augments virion infectivity in a manner that depends on the flexible loop in the absence of CD4 (29), it is possible that the binding of the flexible loop of Nef to GagPol is critical for this effect. Whether Nef still internalizes CD4 when associated with p6* will have to be determined.…”
mentioning
confidence: 99%