Negative Association of Serum Uric Acid with Peripheral Blood Cellular Aging Markers

Yu, Jie; Liu, Hongxing; He, Song; Li, P.; Ma, Chunxiao; Ping, Fan; Zhang, H.; Li, W.; Sun, Qi; Ma, Miling; Liu, Y.; Lv, Le; Xu, Lingling; Li, Yuxiu

doi:10.1007/s12603-019-1200-3

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Our previous studies described leukocyte mtDNAcn analysis in detail (21,23). In brief, genomic DNA was extracted from leukocytes by QIAamp DNA Blood Midi Kits (Qiagen, Hilden, Germany).…”

Section: Measurement Of Mtdnacnmentioning

confidence: 99%

Association Between Leukocyte Mitochondrial DNA Copy Number and Non-alcoholic Fatty Liver Disease in a Chinese Population Is Mediated by 8-Oxo-2′-Deoxyguanosine

Liu

et al. 2020

Front. Med.

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“…Our previous studies described leukocyte mtDNAcn analysis in detail (21,23). In brief, genomic DNA was extracted from leukocytes by QIAamp DNA Blood Midi Kits (Qiagen, Hilden, Germany).…”

Section: Measurement Of Mtdnacnmentioning

confidence: 99%

Association Between Leukocyte Mitochondrial DNA Copy Number and Non-alcoholic Fatty Liver Disease in a Chinese Population Is Mediated by 8-Oxo-2′-Deoxyguanosine

Liu

et al. 2020

Front. Med.

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“…Telomere shortening is associated with a series of age-related deteriorations and diseases, such as cardiovascular ( 22 ) and metabolic diseases ( 23 ). Interestingly, a significant negative association between serum urate and peripheral blood aging markers, including leukocyte telomere length (LTL) and mitochondrial DNA copy number (mtDNAcn) ( 24 ), has indicated that serum urate may play a role in promoting organismal aging by telomere shortening. Although reduced telomere length and elevated serum urate levels both come with aging, a recent Mendelian randomization (MR) study does not support the casual effect of serum urate on telomere shorten ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Associations between serum urate and telomere length and inflammation markers: Evidence from UK Biobank cohort

Cui²,

Zhang³

2022

Front. Immunol.

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ObjectiveHyperuricemia and gout have become gradually more common. The effect of serum urate on organism aging and systematic inflammation is not determined. This study aims to evaluate whether serum urate is causally associated with cellular aging markers and serum inflammation markers.MethodsA Mendelian randomization study was performed on summary-level data from the largest published genome-wide association studies. Single nucleotide polymorphisms with a genome-wide significance level were selected as instrumental variables for leukocyte telomere length (LTL), and serum soluble makers of inflammation (CRP, IL-6, TNF-α, and IGF-1). Standard inverse variance weighted (IVW) method was used as the primary statistical method. The weighted median, MR-Egger regression, and MR-PRESSO methods were used for sensitivity analysis.ResultsAn inverse causal association of genetically predicted serum urate levels and LTL was found using IVW method (OR: 0.96, 95%CI 0.95, 0.97; β=-0.040; SE=0.0072; P=4.37×10-8). The association was also supported by MR results using MR-Egger method and weighted median method. The MR-PRESSO analysis and leave-one-out sensitivity analysis supported the robustness of the combined results. In terms of other aging-related serum biomarkers, there was no evidence supporting a causal effect of serum urate on CRP, IL-6, TNF-α, or IGF-1 levels.ConclusionsSerum urate levels are negatively associated with telomere length but are not associated with serum soluble indicators of inflammation. Telomere length may be a critical marker that reflects urate-related organismal aging and may be a mechanism in the age-related pathologies and mortality caused by hyperuricemia.

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“…Leukocyte DNA was extracted by the QIAamp DNA blood mid kit (Qiagen, Hilden, Germany). The peripheral blood mtDNAcn and LTL measurement techniques were used as elaborated by previous research [18, 26]. LTL was determined as the relative ratio of telomere repeat copy number to single gene copy number using the novel monochrome multiplex quantitative PCR method [27].…”

Section: Methodsmentioning

confidence: 99%

Negative Association between Caloric Intake and Estimated Glomerular Filtration Rate in a Chinese Population: Mediation Models Involving Mitochondrial Function

et al. 2020

Gerontology

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Background: With the shortening of leukocyte telomere length (LTL) and decrease in the copy number of mitochondrial DNA, mitochondrial dysfunction and oxidative stress are considered important drivers of the aging process. Although previous experimental studies report that caloric intake is associated with age-related renal dysfunction through the changes in mitochondrial function, there are insufficient epidemiological data to establish this association. Objective: We aimed to explore the association between caloric intake and renal function and to investigate whether mitochondrial DNA copy number (mtDNAcn) mediated this association by cross-sectional analysis. Methods: A total of 403 individuals from a Chinese rural cohort (women = 66.50%; mean age = 53.94 ± 10.27 years) with an estimated glomerular filtration rate (eGFR) ≥60 mL/min/1.73 m 2 and with differences in the glucose tolerance status (diabetes, n = 106; prediabetes, n = 125; normal glucose tolerance, n = 172) were included. Dietary data were obtained by a 24-h food recall, and caloric intake was normalized by ideal body weight. The mtDNAcn and LTL were detected using realtime PCR assay. The associations between caloric intake, aging markers, and renal function were analyzed by partial correlation analysis and multiple linear regression analysis. Mediation analysis was applied to examine the role of mtDNAcn in the association between caloric intake and eGFR. Results: Caloric intake was higher while age-adjusted mtDNAcn was lower in individuals with eGFR < 90 mL/ min/1.73 m 2 (n = 140) than in those with eGFR ≥90 mL/ min/1.73 m 2 (n = 263). After adjusting for multiple factors, linear regression analysis revealed that caloric intake was negatively associated with eGFR and mtDNAcn, while mtDNAcn was positively associated with eGFR. Moreover, mediation analysis indicated that the indirect effect of caloric intake on eGFR through mtDNAcn was significant (β =-0.0505, 95% confidence interval-0.0931 to-0.0190). Conclusions: Caloric intake was negatively associated with eGFR in a Chinese population, and the association was partly mediated by decreased mtDNAcn.

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Negative Association of Serum Uric Acid with Peripheral Blood Cellular Aging Markers

Cited by 9 publications

References 17 publications

Association Between Leukocyte Mitochondrial DNA Copy Number and Non-alcoholic Fatty Liver Disease in a Chinese Population Is Mediated by 8-Oxo-2′-Deoxyguanosine

Association Between Leukocyte Mitochondrial DNA Copy Number and Non-alcoholic Fatty Liver Disease in a Chinese Population Is Mediated by 8-Oxo-2′-Deoxyguanosine

Associations between serum urate and telomere length and inflammation markers: Evidence from UK Biobank cohort

Negative Association between Caloric Intake and Estimated Glomerular Filtration Rate in a Chinese Population: Mediation Models Involving Mitochondrial Function

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