Negative chemical ionization gas chromatography/mass spectrometry to quantify urinary 3-methylhistidine: Application to burn injury

Beffa, David C.; Carter, Edward A.; Lu, Xiaoming; Yu, Yong‐Ming; Prelack, Kathy; Sheridan, Robert L.; Young, Vernon R.; Fischman, Alan J.; Tompkins, Ronald G.

doi:10.1016/j.ab.2006.03.057

Cited by 16 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its sensitivity allows for the detection of 6.8 pmol of urinary 3MH with a precision of 9.8%. 10 The mean 3MH obtained by averaging the three consecutive 24-hour urine samples was used for calculating skeletal muscle protein breakdown. Skeletal muscle protein breakdown was estimated using a factor of 4.2 µmol 3MH per 1 g of mixed protein.…”

Section: Methodsmentioning

confidence: 99%

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

Prelack

Dylewski

et al. 2010

Journal of Burn Care & Research

Self Cite

View full text Add to dashboard Cite

The physiologic response to trauma results in the efflux of large amounts of amino acids from skeletal muscle. This is extreme in large burn injuries. Protein kinetic studies, although useful in determining the rates of protein synthesis and breakdown, do not provide information about muscle loss. This study determined the contribution of muscle protein to whole-body protein breakdown in children throughout their course of burn injury. Children aged 0 to 18 years with initial burn size ≥30% TBSA underwent 15N glycine and 3 methylhistidine (3MH) analysis during three phases of care: A, early acute; B, wound closure; and C, convalescence. Muscle protein breakdown was estimated using a factor of 4.2 µmol 3MH per 1 g of mixed protein. Twenty-two patients with a mean of 54.5 ± 20.1% TBSA burn were studied. Protein balance did not change remarkably and remained positive by 2 g/kg during hospitalization. However, muscle protein breakdown dropped from 1.1 to 0.6 g/kg with wound closure (P < .0001), representing a decrease in the contribution of muscle protein to whole-body protein breakdown from 20 to 7%. Ten patients returned for a third measurement after discharge. Although protein turnover was high, muscle breakdown was consistent with 3MH values reported in healthy children. Serial determination of 3MH excretion is a simple way to track muscle catabolism throughout burn injury. Our data suggest that despite accelerated protein turnover, muscle catabolism significantly decreases with wound closure and begins to normalize around discharge. In convalescence, 3MH excretion is comparable with normal children.

show abstract

Section: Methodsmentioning

confidence: 99%

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

Prelack

Dylewski

et al. 2010

Journal of Burn Care & Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…A clinical study indicated that urine 3-MH is significantly increased in burned children [27]. It is seen as an important marker of catabolic metabolism.…”

Section: Discussionmentioning

confidence: 99%

Use of 1H-nuclear magnetic resonance to screen a set of biomarkers for monitoring metabolic disturbances in severe burn patients

et al. 2014

View full text Add to dashboard Cite

IntroductionTo establish a plasma metabolomics fingerprint spectrum for severe burn patients and to use it to identify a set of biomarkers that could be used for clinical monitoring.MethodsTwenty-one severe burn patients and three healthy control individuals were enrolled in this study, and the plasma samples from patients and healthy individuals were collected for nuclear magnetic resonance (NMR) measurements. The NMR spectra were analyzed using principal component analysis (PCA) and partial least squares (PLS) in order to establish the metabolomics fingerprint representing the changes in metabolism and to select the major biomarkers.ResultsNMR spectra of the plasma samples showed significant differences between burn patients and healthy individuals. Using metabolomics techniques, we found an Eigen-metabolome that consists of 12 metabolites, which are regulated by 103 enzymes in a global metabolic network. Among these metabolites, α-ketoisovaleric acid, 3-methylhistidine, and β-hydroxybutyric acid were the most important biomarkers that were significantly increased during the early stage of burn injury. These results suggest that the mitochondrial damage and carbohydrate, protein and fatty acid metabolism disturbances occur after burn injury. Our analysis also show that histone deacetylases, which are protein transcription suppressors, were remarkably increased and indicate that protein transcription was inhibited and anabolism was restrained during the early stage of burn injury.ConclusionsMetabolomics techniques based on NMR can be used to monitor metabolism in severe burn patients. Our study demonstrates that integrated 1H-NMR metabolome and global metabolic network analysis is useful for visualizing complex metabolic disturbances after severe burn injury and may provide a new quantitative injury severity evaluation for future clinical use.Trial registrationChinese Clinical Trial Registry ChiCTR-OCC-12002145. Registered 25 April 2012.

show abstract

“…Spot urine samples were collected at the time of urinary catheter insertion in the operating room (preoperative) and at the conclusion of 24‐hour urine collection on the second postoperative day. The concentration of urinary 3‐methylhistidine (3MH) was measured with an Agilent/HP6890 gas chromatographer/mass spectrometer according to previously published procedures . Urinary creatinine (Cr) concentrations were measured with a commercially available Creatinine Assay Kit (Sigma, St Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Whole Body Protein Turnover and Net Protein Balance After Pediatric Thoracic Surgery: A Noninvasive Single‐Dose ¹⁵N Glycine Stable Isotope Protocol With End‐Product Enrichment

Fullerton

Sparks

Khan

et al. 2017

J Parenter Enteral Nutr

Self Cite

View full text Add to dashboard Cite

show abstract

Negative chemical ionization gas chromatography/mass spectrometry to quantify urinary 3-methylhistidine: Application to burn injury

Cited by 16 publications

References 14 publications

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

Use of 1H-nuclear magnetic resonance to screen a set of biomarkers for monitoring metabolic disturbances in severe burn patients

Whole Body Protein Turnover and Net Protein Balance After Pediatric Thoracic Surgery: A Noninvasive Single‐Dose ¹⁵N Glycine Stable Isotope Protocol With End‐Product Enrichment

Contact Info

Product

Resources

About

Negative chemical ionization gas chromatography/mass spectrometry to quantify urinary 3-methylhistidine: Application to burn injury

Cited by 16 publications

References 14 publications

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

The Contribution of Muscle to Whole-Body Protein Turnover Throughout the Course of Burn Injury in Children

Use of 1H-nuclear magnetic resonance to screen a set of biomarkers for monitoring metabolic disturbances in severe burn patients

Whole Body Protein Turnover and Net Protein Balance After Pediatric Thoracic Surgery: A Noninvasive Single‐Dose 15N Glycine Stable Isotope Protocol With End‐Product Enrichment

Contact Info

Product

Resources

About

Whole Body Protein Turnover and Net Protein Balance After Pediatric Thoracic Surgery: A Noninvasive Single‐Dose ¹⁵N Glycine Stable Isotope Protocol With End‐Product Enrichment