Negative correlations between cultivable and active-yet-uncultivable pyrene degraders explain the postponed bioaugmentation

Jiang, Bo; Chen, Yating; Xing, Yi; Lian, Luning; Shen, Yaoxin; Zhang, Baogang; Zhang, Han; Sun, Guangdong; Li, Junyi; Wang, Xinzi; Zhang, Dayi

doi:10.1016/j.jhazmat.2021.127189

Cited by 22 publications

(9 citation statements)

References 94 publications

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“…Therefore they could be captured by an external permanent magnet in MFC_CPF fractions. 15,18 Therefore, the OTUs enriched in MFC_CPF were possibly responsible for the in situ CPF degradation. The taxonomic analysis of the community profiles in MMI_CPF and MFC_CPF on the genus level at different time points is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore they could be captured by an external permanent magnet in MFC_CPF fractions. 15,18 Therefore, the OTUs enriched in MFC_CPF were possibly responsible for the in situ CPF degradation. The taxonomic analysis of the community The NMDS score plot illustrated the cluster of microbial community composition in all treatments, in which the bacterial communities in MMI_CPF and MFC_CPF were clustered together, and exhibited dramatic shis from the original soils (Fig.…”

Section: Microorganisms Responsible For Cpf Biodegradation Identied ...mentioning

confidence: 99%

“…16,17 More recently, Jiang et al applied MMI to isolated active pyrene degraders and found that the bioaugmentation performance was related to the correlations between cultivable and active but not cultivable degraders. 18 This study links indigenous bacterial taxa with the involvement of CPF biodegradation. Combined with high-throughput sequencing, MMI was applied to reveal the active CPF degradants in agricultural soils.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

Lian

Xing

Zhang

et al. 2022

Environ. Sci.: Processes Impacts

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“…Therefore they could be captured by an external permanent magnet in MFC_CPF fractions. 15,18 Therefore, the OTUs enriched in MFC_CPF were possibly responsible for the in situ CPF degradation. The taxonomic analysis of the community profiles in MMI_CPF and MFC_CPF on the genus level at different time points is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore they could be captured by an external permanent magnet in MFC_CPF fractions. 15,18 Therefore, the OTUs enriched in MFC_CPF were possibly responsible for the in situ CPF degradation. The taxonomic analysis of the community The NMDS score plot illustrated the cluster of microbial community composition in all treatments, in which the bacterial communities in MMI_CPF and MFC_CPF were clustered together, and exhibited dramatic shis from the original soils (Fig.…”

Section: Microorganisms Responsible For Cpf Biodegradation Identied ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

Lian

Xing

Zhang

et al. 2022

Environ. Sci.: Processes Impacts

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“…PCR was performed in a thermal cycler (2720, Applied Biosystems, USA) with the following parameters: 95 °C for 3 min, 95 °C for 30 s, 54 For soil microbial communities, soil genomic DNA was amplified for V3−V4 regions of 16S rRNA genes with the universal primer set of 515F (5′-GTGCCAGCMGCCGCGG-TAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). 37 An Illumina Miseq platform was used for sequencing. After removing the primer connector, all paired reads were merged and quality-checked.…”

Section: Soilmentioning

confidence: 99%

Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils

Zhao,

Zhang,

Liang

et al. 2023

Environ. Sci. Technol.

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Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.

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Linking soil characteristics, rhizosphere microbiome composition, and plant defence reaction to apple replant disease severity

Orth,

Krueger,

Liu

et al. 2024

Plant Soil

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Introduction Apple replant disease (ARD) causes reduced growth and fruit yield and affects orchards and tree nurseries worldwide. A number of pathogens have been consistently identified as causal agents of ARD; however factors affecting disease-severity are not fully understood. Aims We examined five soils from German tree nurseries and apple orchards featuring different soil characteristics and replant histories. We aimed to link the plant-soil interaction to replant disease severity. Methods In a greenhouse experiment, young apple plants were grown for eight weeks on untreated and disinfected (control) soils. Growth parameters were recorded to evaluate the severity of ARD. The defence response of the plants was examined by expression analysis of ARD indicator genes (BIS3, B4H and ERF1B) and GC–MS-based detection of phytoalexins. The fungal and bacterial rhizosphere communities were investigated by ITS and 16S rRNA amplicon sequencing, respectively. Results After eight weeks, ARD symptoms were observed on all soils. Growth depression was highest on soils that had faced intensive apple cultivation and lowest on a soil with only one year of apple cultivation prior to the experiment. These results correlated with increases in the BIS3 expression level and the phytoalexin content in the roots. No bacteria and fungi commonly found in increased abundance in ARD soils were consistently detected in all soils. Conclusions Replant history influenced disease severity more than soil characteristics. ARD symptoms correlated with BIS3 expression and phytoalexin (PA) formation. PA exudation increased the relative abundance of bacterial genera with the potential ability to degrade phenolic compounds.

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Negative correlations between cultivable and active-yet-uncultivable pyrene degraders explain the postponed bioaugmentation

Cited by 22 publications

References 94 publications

Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

Identification of chlorpyrifos-degrading microorganisms in farmland soils via cultivation-independent and -dependent approaches

Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils

Linking soil characteristics, rhizosphere microbiome composition, and plant defence reaction to apple replant disease severity

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