Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role of blue light emitting diodes

Yuan, Shu; Lv, Zhenming; Zhu, Aiyi; Zheng, Jia; Wu, Changwen

doi:10.1016/j.ecoenv.2017.01.021

Cited by 48 publications

(11 citation statements)

References 44 publications

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“…The Cd is a genotoxic metal for fish [70] that has already been classified as a carcinogen in humans, and is considered toxic to aquatic organisms by inducing the oxidative stress and immunotoxicity. The level of Cd in water can reach 0.05 mg•L −1 [71]. The mean Cd concentration in the muscle of turbot is similar to the results reported by Ergönül and Altindag [40], Nisbet et al [39] and Bat et al [37].…”

Section: Resultssupporting

confidence: 87%

Bioconcentration of Essential and Nonessential Elements in Black Sea Turbot (Psetta Maxima Maeotica Linnaeus, 1758) in Relation to Fish Gender

Simionov

Cristea

Petrea

et al. 2019

JMSE

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This study investigates the influence of gender in the bioconcentration of essential and nonessential elements in different parts of Black Sea turbot (Psetta maxima maeotica) body, from an area considered under high anthropogenic pressure (the Constanta City Black Sea Coastal Area in Romania). A number of 13 elements (Ca, Mg, Na, K, Fe, Zn, Mn, Cu, Ni, Cr, As, Pb and Cd) were measured in various sample types: muscle, stomach, stomach content, intestine, intestine content, gonads, liver, spleen, gills and caudal fin. Turbot adults (4–5 years old) were separated, according to their gender, into two groups (20 males, 20 females, respectively), and a high total number of samples (1200 from both groups) were prepared and analyzed, in triplicate, with Flame Atomic Absorption Spectrometry and High-Resolution Continuum Source Atomic Absorption Spectrometry with Graphite Furnace techniques. The results were statistically analyzed in order to emphasize the bioconcentration of the determined elements in different tissues of wild turbot males vs. females, and also to contribute to an upgraded characterization of the Romanian Black Sea Coast, around Constanta City, in terms of heavy metals pollution. The essential elements Mg and Zn have different roles in the gonads of males and females, as they were the only elements with completely different patterns between the analyzed groups of specimens. The concentrations of studied elements in muscle were not similar with the data provided by literature, suggesting that chemistry of the habitat and food plays a major role in the availability of the metals in the body of analyzed fish species. The gender influenced the bioaccumulation process of all analyzed elements in most tissues since turbot male specimens accumulated higher concentration of metals compared to females. The highest bioaccumulation capacity in terms of Ca, Mg, Na, Ni, As, Zn and Cd was registered in caudal fin, liver and intestine tissues. Also, other elements such as K, Fe, Cu and Mn had the highest bioaccumulation in their muscle, spleen, liver and gills tissues. The concentrations of toxic metals in Black Sea turbot from this study were lower in the muscle samples compared with the studies conducted in Turkey, suggesting that the anthropogenic activity in the studied area did not pose a major impact upon the habitat contamination.

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Section: Resultssupporting

confidence: 87%

Bioconcentration of Essential and Nonessential Elements in Black Sea Turbot (Psetta Maxima Maeotica Linnaeus, 1758) in Relation to Fish Gender

Simionov

Cristea

Petrea

et al. 2019

JMSE

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“…Cadmium (Cd) is toxic to aquatic organisms, and it can have long-term adverse effects on the aquatic environment (Rajan et al, 1995;Čelechovská et al, 2007;Vinodhini and Narayanan, 2008;Chen et al, 2013;Yuan et al, 2017;Liu et al, 2018). In animals, Cd is mainly enriched in the liver and kidney (Dudley et al, 1982;Thijssen et al, 2007;Włostowski et al, 2008;Elboshy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

et al. 2021

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Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.

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“…The molecular mechanisms of cold stress have well been elucidated by RNA-seq in zebra sh larvae (Long et al, 2013;. Our studies have suggested that blue LEDs (LDB) play a positive role in immune and antioxidant systems (Zheng et al, 2016a;Yuan et al, 2017a), and mitigated cadmium-induced toxicity in the liver of zebra sh (Yuan et al, 2017b). To test the hypothesis that LDB can protect sh against cold shock in sh, zebra sh were pre-exposed to white LEDs and blue LEDs for 2 weeks, and then exposed to 26℃ or 11℃, respectively.…”

Section: Introductionmentioning

confidence: 91%

“…The pre-acclimation to LDB for 2 weeks was selected because it could induce sh resistance to cold shock. The setting of irradiance is determined according to our previous research (Yuan et al, 2017b;Zheng et al, 2016a). The low temperature at 11°C was selected for the cold shock experiment because sh started to die at this temperature after 12 h. The low water temperature was maintained by a constant circulation of cold water by using a thermostat.…”

Section: Experimental Designmentioning

confidence: 99%

Cold Shock Induced Oxidative Stress, Apoptosis and Genome-wide Gene Expression Perturbation in the Eyes of Zebrafish and the Mitigation of Blue Wavelength Light

Peng

Han

Wen

et al. 2021

Preprint

Self Cite

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As global climate changes, severely temperature variations have significant impacts on survival and development of fish. While the potential effects of light wavelength on cold shock and associated mechanisms remain largely unknown in fish. Here, zebrafish were pre-exposed to white LEDs (an irradiance of 0.9 W/m2) and blue LEDs (LDB, peak at 450 nm, 0.9 W/m2) for 2 weeks, and then exposed to 26℃ or 11℃ for 48 hours, respectively. Cold shock led to low survival rate. Cold shock altered retinal structure, increased the number of apoptotic cells and Caspase-3 activity, inhibited superoxide dismutase (SOD) and catalase (CAT) activities, up-regulated mRNA expression of (NF-E2-related factor 2) nrf2, p53, casp3 and casp9, and down-regulated cat expression in fish eyes. These results demonstrated that acute cold exposure induced oxidative stress and apoptosis in zebrafish eyes, which may lead to mortality. However, cold shock in combination with LDB apparently mitigated these negative effects, which might be involved in the up-regulation of antioxidant response and down-regulation of apoptotic responses at transcriptional and translational levels. Furthermore, cold shock also caused dysregulation of genome-wide gene expression involved in circadian rhythm, phototransduction and il-17 signaling pathway, indicating that cold shock disturbed phototransduction cascade and circadian rhythm signals and caused inflammatory responses. Ten key genes involved in circadian rhythm, phototransduction, cell cycle arrest, RNA processing or inflammatory responses were identified, including muc5d, rnps1, si:dkey-243i1.1, opn1mw1, gadd45ba, cebpd, btg2, si:dkey-242g16.2, nr1d1 and zgc:122979, which may play an important role in the protection of LDB against cold shock. Finally, our study suggested the relationship between spectrum and cold stress and demonstrated LDB could protect fish against the negative effect of cold stress in the eyes.

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Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role of blue light emitting diodes

Cited by 48 publications

References 44 publications

Bioconcentration of Essential and Nonessential Elements in Black Sea Turbot (Psetta Maxima Maeotica Linnaeus, 1758) in Relation to Fish Gender

Bioconcentration of Essential and Nonessential Elements in Black Sea Turbot (Psetta Maxima Maeotica Linnaeus, 1758) in Relation to Fish Gender

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

Cold Shock Induced Oxidative Stress, Apoptosis and Genome-wide Gene Expression Perturbation in the Eyes of Zebrafish and the Mitigation of Blue Wavelength Light

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