Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria

Zhu, Zhendong; Kawai, Tomoko; Umehara, Takashi; Hoque, S. A. Masudul; Zeng, Wenxian; Shimada, M.

doi:10.1016/j.freeradbiomed.2019.06.018

Cited by 94 publications

(67 citation statements)

References 62 publications

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“…Sperm motility was evaluated via a computer-assisted sperm analysis (CASA) system (HVIEW, Fuzhou, China) every two days from the first day of storage. Briefly, samples were transferred into a water bath at 37 °C for 10 min, then 6 μL semen were dropped onto a pre-heated glass slide (37 °C; 20 μm CELL-VU ® DRM-600 sperm count slide; Millennium Sciences, NewYork, NY, USA) for evaluation according to Zhu et al [ 20 ]. The standard parameter settings in this experiment were 30 frames/s.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, sperm is prone to lipid peroxidation (LPO) [ 6 , 16 ] and DNA damage [ 16 , 17 ] under oxidative stress. Moreover, excessive ROS damages the membrane structure and causes irreversible loss of motility [ 18 , 19 , 20 ]. Thus, maintaining the balance of ROS generation and scavenging is essential to maintain cell structure and function.…”

Section: Introductionmentioning

confidence: 99%

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Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Feng

Zhu

Bai

et al. 2020

Animals

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Proline was reported to improve sperm quality in rams, stallions, cynomolgus monkeys, donkeys, and canines during cryopreservation. However, the underlying mechanism remains unclear. The aim of this study was to investigate the effect of proline on boar semen during liquid storage at 17 °C and explore the underlying mechanism. Freshly ejaculated boar semen was supplemented with different concentrations of proline (0, 25, 50, 75, 100, 125 mM) and stored at 17 °C for nine days. Sperm motility patterns, membrane integrity, ATP (adenosine triphosphate), reactive oxygen species (ROS), and GSH (glutathione) levels, and the activities of catalase (CAT) and superoxide dismutase (SOD) were evaluated after storage for up to five days. It was observed that boar sperm quality gradually decreased with the extension of storage time, while the ROS levels increased. Addition of 75 mM proline not only significantly improved sperm membrane integrity, motility, and ATP levels but also maintained the redox homeostasis via increasing the GSH levels and activities of CAT and SOD. When hydrogen peroxide (H2O2) was used to induce oxidative stress, addition of proline significantly improved sperm quality and reduced ROS levels. Moreover, addition of proline also improved sperm quality during the rapid cooling process. Notably, addition of DL-PCA (DL-pipecolinic acid) rescued the reduction of progressive motility and total motility caused by H2O2, and THFA (tetrahydro-2-furoic acid) failed to provide protection. Furthermore, addition of proline at 75 mM increased the activity of proline dehydrogenase (PRODH) and attenuated the H2O2-induced reduction in progressive motility. These data demonstrate that proline protects sperm against oxidative stress through the secondary amine structure and proline dehydrogenase-mediated metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Feng

Zhu

Bai

et al. 2020

Animals

Self Cite

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“…Interestingly, they also showed that treatment with PQQ and CoQ10 increased mitochondrial DNA (mt-DNA) stability and countered the damage caused by ROS to the specific RNA polymerase (POLMRT) and the mitochondrial transcription factor-A (TFAM) that are fundamental for mitochondrial transcription. These results suggested that a mitochondrion-targeted antioxidant treatment could improve sperm linear motility by preserving the sperm transcription system from ROS damage [66].…”

Section: Effects Of Prokinetic/antioxidant Therapy On Sperm Mitochondmentioning

confidence: 83%

Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility

Barbagallo

Vignera

Cannarella

et al. 2020

JCM

117

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Introduction: The role of nutraceuticals in the treatment of male infertility, especially in the “idiopathic form”, remains the subject of significant debate. Many antioxidants improve sperm motility but the exact mechanism by which they act is still unclear. Although several studies have shown a correlation between sperm motility and mitochondrial function, the effects of antioxidant therapy on mitochondrial membrane potential (MMP) are poorly studied. The first aim of this review was to evaluate the efficacy of antioxidants on mitochondrial function and, consequently, on sperm motility in male infertile patients. Material and Methods: we performed a systematic search of all randomized controlled and uncontrolled studies available in the literature that reported sperm motility and MMP at baseline and after antioxidant administration in-vivo and in-vitro in patients with idiopathic asthenozoospermia. Pubmed, MEDLINE, Cochrane, Academic One Files, Google Scholar and Scopus databases were used. Results: Unexpectedly, among 353 articles retrieved, only one study met our inclusion criteria and showed a significant effect of myoinositol on both MMP and sperm motility. We then summarized the main knowledge on anatomy and metabolism of sperm mitochondria, techniques allowing to assess sperm mitochondria function and its relationships with low sperm motility. Finally, we paid special attention to the effect of antioxidant/prokinetic molecules for the treatment of asthenozoospermia. Conclusions: This is the first systematic review that has attempted to evaluate the effects of antioxidants on MMP and sperm motility. Although results are not conclusive due to the dearth of studies, the close relationship between mitochondria and sperm motility is clear. The investigation of this correlation could provide valuable information to be exploited in clinical practice for the treatment of male infertility.

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“…Effect of H 2 O 2 on hierarchical clustering analysis in H 2 O 2 -treated human melanocytes and untreated controls the oviduct. [32 During T-cell migration and differentiation, mitochondrial ATP is needed to fulfil the energy needs. A decrease of ATP production may comprise immune response and trigger autoimmune disease or inflammation.…”

Section: Discussionmentioning

confidence: 99%

H₂O₂‐induced oxidative stress disrupts mitochondrial functions and impairs migratory potential of human epidermal melanocytes

Xue

et al. 2020

Experimental Dermatology

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Reactive oxygen species (ROS) have already been demonstrated to impede the migratory ability in non‐melanocytic cell lines by depleting mitochondrial ATP production. Therefore, understanding the mitochondrial metabolic response to migration in the presence of ROS should be a key to understanding repigmentation in vitiligo. This study aimed to investigate the energy mechanism associated with the ROS‐mediated attenuation of melanocyte migration. After melanocytes were pretreated with H2O2, their ATP production, migratory ability, ultrastructural changes and Mitochondrial Permeability Potential were analysed. The results showed that, in parallel with the decreased ATP production, the migratory ability of melanocytes was significantly inhibited by oxidative stress. Supplementation with exogenous ATP reversed the suppressed ATP‐dependent migration of melanocytes. Melanocytes were then stressed with H2O2 and Agilent Whole Human Genome microarray analysis identified 763 up‐regulated mRNAs and 1117 down‐regulated mRNAs. Among them, 11 of the encoded proteins were involved in mitochondrial ATP production and their expression levels were verified. The decreased expression of NADH dehydrogenase 2(ND2), cytochrome c oxidase 1(COX1) and cytochrome c oxidase 3(COX3) was shown to be involved in the depletion of mitochondrial ATP production, which was coupled with the impaired migratory potential. These results indicate that the migration of melanocytes relies heavily on an inexhaustible supply of ATP from mitochondria.

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Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria

Cited by 94 publications

References 62 publications

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility

H₂O₂‐induced oxidative stress disrupts mitochondrial functions and impairs migratory potential of human epidermal melanocytes

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Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria

Cited by 94 publications

References 62 publications

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine

Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility

H2O2‐induced oxidative stress disrupts mitochondrial functions and impairs migratory potential of human epidermal melanocytes

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Product

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H₂O₂‐induced oxidative stress disrupts mitochondrial functions and impairs migratory potential of human epidermal melanocytes