Negative Electric Potential Induces Alteration of Ion Gradient and Lamellar Body Secretion in the Epidermis, and Accelerates Skin Barrier Recovery After Barrier Disruption

Denda, Mitsuhiro; Kumazawa, Noriyuki

doi:10.1046/j.0022-202x.2001.01500.x

Cited by 38 publications

(39 citation statements)

References 43 publications

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“…It has been also reported that barrier disruption, hallmark of AD, was related to the decrease of calcium, magnesium, potassium, and zinc in in vitro and animal studies (Denda et al, 2000;Denda and Kumazawa, 2002;Makiura et al, 2004), although these associations are not clear in human studies (Tricon et al, 2006). Like zinc shown in Table 2, when we analyzed our data regarding the intakes of calcium and potassium, there was no statistically significant association between these nutrients and AD in this study (data not shown).…”

Section: Discussionmentioning

confidence: 41%

Antioxidant nutrient intakes and corresponding biomarkers associated with the risk of atopic dermatitis in young children

Chung

Kim

et al. 2010

Eur J Clin Nutr

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Background/Objectives: To investigate the association of antioxidant nutritional status with the risk of atopic dermatitis (AD) in young children in a case-control, population-based study. Subjects/Methods: Identified from preschools by using the Korean version of the International Study of Asthma and Allergies in Childhood (ISAAC). Final analysis included 180 AD (mean age 5.3 ± 0.9 years) and 242 non-AD (mean age 5.2 ± 1.0 years) children. Diet was assessed using a validated semi-quantitative food frequency questionnaire. Fasting blood samples were used for analyses of fat-soluble vitamins (retinol, a-tocopherol, and b-carotene) and vitamin C. . There was no relationship of AD risk with dietary and plasma vitamin C as well as nutrient supplement intake regardless of nutrient type. AD was predicted better by the intake measure than the corresponding blood biomarker regarding vitamin E and b-carotene. Conclusions: These findings suggest that higher antioxidant nutritional status reduces the risk of AD and that such risk-reduction effects depend on nutrient type.

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Section: Discussionmentioning

confidence: 41%

Antioxidant nutrient intakes and corresponding biomarkers associated with the risk of atopic dermatitis in young children

Chung

Kim

et al. 2010

Eur J Clin Nutr

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“…Moreover, in this study, we loaded the electric potential on untreated ex vivo skin, while in our previous mouse study, we had loaded the potential after barrier disruption. Our earlier study suggested that external potential influenced lamellar body secretion during barrier recovery after disruption (1). On the other hand, the present findings suggest that exocytosis of lamellar bodies and fusion of secreted lipids might be regulated by the electro-physiological condition of the epidermis even in normal skin.…”

Section: Resultscontrasting

confidence: 37%

“…Moreover, the application of negative electric potential after barrier disruption increased the calcium ion gradient at the uppermost nucleated layer of the epidermis (1). In this study, we observed calcium ion gradation in the epidermis of control skin and potential-loaded skin by means of two-photon microscopy.…”

Section: Letter To the Editormentioning

confidence: 66%

“…We previously demonstrated that application of an external negative electric potential on hairless mouse skin accelerated recovery of the epidermal permeability barrier after disruption (1). In that study, we found that exocytosis of lamellar bodies at the extracellular lipid domain between stratum corneum and stratum granulosum is accelerated after barrier disruption.…”

Section: Introductionmentioning

confidence: 69%

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External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo

Kumamoto

Goto

Denda

et al. 2013

Experimental Dermatology

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Additional Supporting Information may be found in the online version of this article: Figure S1. Average of the 13 dilipidated SC AFM-IR spectra shown in Fig. 1b (top).Data S1. Additional background and experimental design sections. Abstract: Exocytosis of lamellar bodies at the uppermost nucleated layer of the epidermis is a crucial process for epidermal permeability barrier homoeostasis. We have previously suggested that skin surface electric potential might be associated with barrier homoeostasis. Thus, we hypothesized that the potential might drive exocytosis of lamellar bodies. In this study, we tested this idea by applying negative electric potential (À0.5 V) to human skin samples ex vivo for 2 h and observing the ultrastructure of the uppermost layer. The secretion of lamellar bodies was accelerated in the potential-applied skin, compared to that in untreated control skin. Multiphoton observation indicated that extracellular lipid domains were more extensive in treated skin than in control skin. Moreover, the calcium ion gradient was greater at the uppermost layer of the epidermis of treated skin, compared to that in control skin. These results indicate that electric potential may regulate lamellar body secretion in healthy human skin. DOI

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“…In a preliminary study, we have previously reported a mineral imbalance in AEDS patients (Hanifin & Rajka, 1980;Kimata et al, 2002). Moreover, skin barrier recovery is influenced by the electric charge of minerals in the epithelium (Edelberg, 1977;Denda et al, 2000;Denda & Kumazawa, 2002). Mg deficiency results in AEDS-like symptoms in hairless rats (Neckermann et al, 2000a, b;Makiura et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome

Hataguchi¹,

Tai²,

Nakajima³

et al. 2005

Eur J Clin Nutr

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Objective: The effect of drinking deep-sea water on hair minerals was studied in patients with atopic eczema/dermatitis syndrome (AEDS). Study of hair minerals revealed an imbalance of essential minerals and an increase in toxic minerals in AEDS patients. Design: After drinking deep-sea water (Amami no Mizu) for 6 months in AEDS patients, hair minerals (essential minerals and toxic minerals), clinical evaluation of the skin symptoms were compared before drinking with after drinking. Subjects: After obtaining informed consent, 33 patients (mean age 26 y, range 1-50 y, 13 male and 20 female subjects) with mild to moderate AEDS were enrolled. Results: After drinking deep-sea water, the levels of the essential mineral, potassium (K), were significantly decreased, while the levels of selenium (Se) increased. On the other hand, drinking deep-sea water significantly decreased the levels of the toxic minerals, mercury and lead. Moreover, after drinking deep-sea water, the skin symptoms were improved in 27 out of 33 patients. Conclusion: These results indicate that the mineral abnormalities/imbalance may be involved in the pathogenesis of AEDS, and that drinking deep-sea water may be useful in the treatment of AEDS.

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Negative Electric Potential Induces Alteration of Ion Gradient and Lamellar Body Secretion in the Epidermis, and Accelerates Skin Barrier Recovery After Barrier Disruption

Cited by 38 publications

References 43 publications

Antioxidant nutrient intakes and corresponding biomarkers associated with the risk of atopic dermatitis in young children

Antioxidant nutrient intakes and corresponding biomarkers associated with the risk of atopic dermatitis in young children

External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo

Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome

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