Limiting global warming to 1.5 °C requires negative emission technologies (NETs), which remove carbon dioxide from the atmosphere and permanently store it to offset unavoidable emissions. Successful large-scale deployment of NETs depends not only on technical, biophysical, ecological, and economic factors, but also on public perception and acceptance. However, previous studies on this topic have been scarce. In 2019, Switzerland adopted a net zero greenhouse gas emissions by 2050 target, which will require the use of NETs. To examine the current Swiss public perception and acceptance of five different NETs, we conducted an online survey with Swiss citizens (N = 693). By using a between-subjects design, we investigated differences in public opinion, perception, and acceptance across three of the most used frames in the scientific literature — technological fix, moral hazard, and climate emergency. Results showed that the public perception and acceptance of NETs does not differ between the frames. The technological fix frame best reflected participants’ opinion, whereas participants perceived the moral hazard frame the least credible and the climate emergency frame the most unclear. Moreover, our findings confirm the public’s unfamiliarity with NETs. We found no strong opposition, as participants indicated a moderate acceptance and a neutral evaluation of all five NETs, with afforestation standing out as the most accepted and positively evaluated NET. We conclude that, in the future, the public debate on NETs should be intensified, and the public perception should be monitored regularly to inform the development of NETs.