Negative regulation of fibrin polymerization and clot formation by nanoparticles of silver

Shrivastava, Siddhartha; Singh, Sarita; Mukhopadhyay, Avijit; Sinha, Asha; Mandal, R.K.; Dash, Debabrata

doi:10.1016/j.colsurfb.2010.08.048

Cited by 39 publications

(30 citation statements)

References 30 publications

(47 reference statements)

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“…Compared to the IR spectrum of CL, the intensity of amide I in CLF was enhanced at 1631 cm −1 after incorporation of fibrinogen [29–30]. It is the major spectral feature in native fibrinogen.…”

Section: Resultsmentioning

confidence: 99%

Novel osteoinductive photo-cross-linkable chitosan-lactide-fibrinogen hydrogels enhance bone regeneration in critical size segmental bone defects

et al. 2014

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The purpose of this study was to develop and characterize a novel photo-cross-linkable chitosan-lactide-fibrinogen (CLF) hydrogel and evaluate the efficacy of bone morphogenetic protein-2 (BMP-2) containing CLF hydrogel for osteogenesis in vitro and in vivo. We synthesized the CLF hydrogels and characterized their chemical structure, degradation rate, compressive modulus, and in vitro BMP-2 release kinetics. We evaluated bioactivities of the BMP-2 containing CLF hydrogels (0, 50, 100, and 500 ng/ml) in vitro using W-20-17 preosteoblast mouse bone marrow stromal cells and C2C12 mouse myoblast cells. The effect of BMP-2 containing CLF gels (0, 0.5, 1, 2, and 5μg) on bone formation was evaluated using rat critical size segmental bone defects for 4 weeks. FTIR spectra and SEM images showed chemical and structural changes by addition of fibrinogen into chitosan-lactide copolymer. Incorporation of fibrinogen molecules significantly increased compressive modulus of the hydrogels. In vitro BMP-2 release study showed initial burst releases from the CLF hydrogels followed by sustained releases, regardless of the concentration of the BMP-2 over 4 weeks. Cells in all groups were viable in the presence of the hydrogels regardless of BMP-2 doses, indicating non-cytotoxicity of hydrogels. Alkaline phosphate activity and mineralization of cells exhibited dose dependence on BMP-2 containing CLF hydrogels. Radiographs, microcomputed tomography, and histology confirmed that the BMP-2 containing CLF hydrogels prompted neo-osteogenesis and accelerated healing of the defects in a dose-dependent manner. Thus the CLF hydrogel is a promising delivery system of growth factors for bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Novel osteoinductive photo-cross-linkable chitosan-lactide-fibrinogen hydrogels enhance bone regeneration in critical size segmental bone defects

et al. 2014

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“…In addition to anti-platelet and antibacterial properties of Ag NPs, an effect on the polymerization of fibrinogen is altering its molecular conformation, and thus its role in blood clotting. A concentration over a threshold value of 25 mg (pure) and 50 mg (plasma), Ag NPs prolong the lag time or even completely inhibit the clotting 21 .…”

Section: Introductionmentioning

confidence: 98%

Comparison of antibacterial activity of Ag nanoparticles synthesized from leaf extract ofParthenium hystrophorusL in aqueous media and Gentamicin sulphate:in-vitro

Anwar¹,

Yadav

Kapoor

et al. 2013

Drug Development and Industrial Pharmacy

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Monodisperse silver (Ag) nanoparticles were synthesized by using Parthenium hystrophorus L leaf extract in aqueous media. The synthesized nanoparticles were characterized by using UV-vis spectrophotometer, X-ray diffracto-meter (XRD), transmission electron microscope (TEM), and dynamics light scattering (DLS). Size-dependent antibacterial activities of Ag nanoparticles were tested against Gram negative Pseudomonas aeruginosa and Gram positive Staphylococcus aureus. Ag nanoparticles having 20 ± 2 nm size in diameter show maximum zone of inhibition (23 ± 2.2 mm) in comparison to 40 nm and 70 nm diameter nanoparticles for Pseudomonas aeruginosa. The zone of inhibition against Staphylococcus aureus were 19 ± 1.8 mm, 15 ± 1.5 mm and 11 ± 1 mm for 20 nm, 40 nm, and 70 nm, respectively. In addition, affect of concentration of 20 nm size Ag nanoparticles on Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus species were also reported and results were compared with 10 µg/ml dose of Gentamicin sulphate. The Parthenium hystrophorus L leaf extract capped 20 ± 2 nm Ag nanoparticles (7.5 µg/ml) shows statistically significant antibacterial activity than Gentamicin sulphate (10 µg/ml) against Staphylococcus aureus.

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“…11,19,20 Nanoparticles were spherical in shape, 10-15 nm in diameter ( Figure 1A), and monodispersed with a narrow particle size distribution ( Figure 1B). The selected area electron diffraction pattern from these particles matched the crystallographic planes of the face-centered cubic AgNPs ( Figure 1C).…”

Section: Resultsmentioning

confidence: 99%

“…11,19,20 In short, 0.017 g silver nitrate was dissolved in deionized water along with sodium hydroxide (0.01 M) and liquid ammonia (2%) to form a 0.01 M solution of stable soluble complex of silver ions. D-glucose and hydrazine (each at 0.01 M concentration) were added to the solution of silver ions to ensure its complete reduction at a final concentration of 0.005 M. The pH of the solution was adjusted to 7.4 with citric acid.…”

Section: Synthesis and Characterization Of Biocompatible Nanoparticlesmentioning

confidence: 99%

Novel microfilaricidal activity of nanosilver

Singh¹,

Goswami²,

Sharma³

et al. 2012

IJN

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Purpose:The currently available drug repertoire against lymphatic filariasis, a major health hazard in the developing world, is inadequate and is fraught with serious limitations. Thus, the development of an effective antifilarial strategy has become a global research thrust mandated by the World Health Organization. Nanoparticles of silver endowed with antibacterial potency are known to induce apoptosis in eukaryotic cells. The present study was designed to investigate the possible microfilaricidal efficacy of silver nanoparticles and to establish the validity of apoptotic rationale in antifilarial drug designing. Methods: This report analyzed the effect of nanoparticles of silver as well as gold (size range: 10-15 nm) on the microfilariae of Brugia malayi obtained from the lavage of peritoneal cavities of infected jirds (Meriones unguiculatus). The study included a microfilarial motility assay, a trypan blue exclusion test, a poly(adenosine diphosphate-ribose) polymerase activity study, ethidium bromide/acridine orange differential staining, and transmission, as well as scanning electron microscopic evaluation of ultrastructural changes in microfilariae. Results: The study demonstrates that nanoparticles of silver, but not of gold, elicited significant loss in microfilarial motility. Differential staining of parasites with ethidium bromide and acridine orange, poly(adenosine diphosphate-ribose) polymerase activity in microfilarial lysate, and electron microscopic findings underscored apoptotic death of parasites attributable to nanosilver. In a trypan blue exclusion test, the 50% lethal dose of nanosilver was measured to be 101.2 µM, which was higher than the recorded complete inhibitory concentration value (50.6 µM), thus supporting nanosilver as a potential drug candidate against lymphatic filariasis. Conclusion:The present report provides the first ever conclusive proof in support of apoptosis as a novel stratagem in antifilarial drug designing and nanoscale silver as a valid lead in research on antifilarial therapeutics. The main embargo about the current drug diethylcarbamazine citrate is its empirical use without rationale. Effective microfilaricidal activity of nanosilver at relatively low concentrations as reported in this study, with evidence of the induction of apoptosis in microfilariae, projects nanosilver as a potential drug adjuvant against lymphatic filariasis. The much higher 50% lethal dose value of nanosilver compared to the complete inhibitory concentration value reported in this study argues in favor of a safe therapeutic window of this agent in its antifilarial efficacy.

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Negative regulation of fibrin polymerization and clot formation by nanoparticles of silver

Cited by 39 publications

References 30 publications

Novel osteoinductive photo-cross-linkable chitosan-lactide-fibrinogen hydrogels enhance bone regeneration in critical size segmental bone defects

Novel osteoinductive photo-cross-linkable chitosan-lactide-fibrinogen hydrogels enhance bone regeneration in critical size segmental bone defects

Comparison of antibacterial activity of Ag nanoparticles synthesized from leaf extract ofParthenium hystrophorusL in aqueous media and Gentamicin sulphate:in-vitro

Novel microfilaricidal activity of nanosilver

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