Negative transcriptional regulation of the human periostin gene by YingYang-1 transcription factor

Romeo, Francesco; Falbo, Lucia; Sanzo, Maddalena Di; Misaggi, R.; Faniello, Maria Concetta; Barni, Tullio; Cuda, Giovanni; Viglietto, Giuseppe; Santoro, Claudio; Quaresima, Barbara; Costanzo, Francesco

doi:10.1016/j.gene.2011.07.025

Cited by 14 publications

(8 citation statements)

References 35 publications

(54 reference statements)

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“…Therefore, it may be possible that stromal fibroblasts are activated by cancer-derived or fibroblast-derived TGF-β in a paracrine or autocrine manner, which leads to the activation of PN production from stromal cells into the tumor microenvironment. Although the reason for the observation of negative-to-low expression of PN in CCA cells has yet to be elucidated, it may be explained by the aberrant PN expression control machinery, particularly, the abnormalities of certain transcription factors (45–47) and microRNAs (miRNAs) (48,49). In addition, the high PN-expressing CCA-associated fibroblasts were identified to have low level of miR-1, whereas those with low PN had high miR-1 expression (unpublished data from our group).…”

Section: Discussionmentioning

confidence: 99%

Clustering of patients with intrahepatic cholangiocarcinoma based on serum periostin may be predictive of prognosis

Thuwajit

Jamjantra

et al. 2017

Oncology Letters

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An effective serum biomarker may improve cholangiocarcinoma (CCA) management. Periostin (PN) has been demonstrated to be associated with aggressive CCA. The current study evaluated PN in blood serum for its diagnostic and prognostic potential in patients with CCA. Sera of 68 patients with CCA were collected prior to treatment, and PN levels were measured using an ELISA. Sera from 50 normal controls, 6 patients with benign liver diseases, 2 with hepatocellular carcinoma and 21 with breast cancer were analyzed. Immunohistochemistry of PN in CCA tissues was also investigated. The data were analyzed using the Mann-Whitney U test, Kaplan-Meier log rank tests, Cox proportional hazard regression models and Fisher's exact tests. The median serum PN level in patients with CCA was significantly increased compared with that in healthy controls, patients with benign liver diseases and patients with breast cancer (all P<0.05). Using an optimal threshold value of 94 ng/ml PN, the diagnostic values for CCA compared with other conditions demonstrated a sensitivity level of 0.38 [95% confidence interval (CI), 0.27–0.51], specificity of 0.90 (95% CI, 0.81–0.96), accuracy of 0.66 (95% CI, 0.58–0.74), positive predictive value of 0.76 (95% CI, 0.59–0.89) and negative predictive value of 0.63 (95% CI, 0.53–0.72) (P<0.001). Furthermore, PN stain in stromal fibroblasts in CCA tissues was associated with serum PN levels (P=0.001), and patients with CCA were classified as low (≤94 ng/ml) or high PN (>94 ng/ml) accordingly. High serum and tissue PN levels were significantly associated with reduced survival rate (P<0.001 and P=0.033, respectively). Serum PN was an independent prognostic factor with a hazard ratio of 3.197 (P=0.001). In conclusion, serum PN may be used to divide patients with intrahepatic CCA into high and low PN groups. Elevated serum PN may be utilized as a marker of poor prognosis in patients with CCA.

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Section: Discussionmentioning

confidence: 99%

Clustering of patients with intrahepatic cholangiocarcinoma based on serum periostin may be predictive of prognosis

Thuwajit

Jamjantra

et al. 2017

Oncology Letters

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“…2B , upper panel). YY1 regulates many genes associated with cell growth and differentiation by binding to a consensus DNA sequence GCCATNTT/AANATGGC of targeted genes 27 28 . Chromatin Immunoprecipitation (ChIP) assays showed that YY1 could bind to the 3 potential binding elements in Sox4 promoter ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Hepatitis B virus replication and sex-determining region Y box 4 production are tightly controlled by a novel positive feedback mechanism

Shang

Zheng

Guo

et al. 2015

Sci Rep

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Hepatitis B virus (HBV) infection is a major cause of liver diseases. However, the mechanisms underlying HBV infection and pathogenesis remain largely unknown. The sex-determining region Y box 4 (Sox4) is a transcriptional factor, which preferentially regulates the development of various organs, tissues, and cancers. But, the role of Sox4 in viral infection and pathogenesis has not been elucidated. Here, we demonstrated that Sox4 is up-regulated by HBV, and revealed the mechanism by which HBV regulates Sox4 expression. First, HBV stimulates Sox4 expression through transcriptional factor Yin Yang 1 (YY1), which binds to Sox4 promoter to activate Sox4 transcriptional activity. Second, miR-335, miR-129-2 and miR-203 inhibit Sox4 expression by targeting its mRNA 3’UTR, while HBV suppresses the microRNAs expression, resulting in up-regulating Sox4 post-transcriptionally. Third, Sox4 protein is degraded by proteasome, while HBV surface protein (HBsAg) prevents Sox4 from degradation by directly interacting with the protein, thereby enhancing Sox4 production post-translationlly. More interestingly, HBV-activated Sox4 in turn facilitates HBV replication by direct binding to the viral genome via its HMG box. Thus, this study revealed a novel positive feedback mechanism by which Sox4 production and HBV replication are tightly correlated.

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“…In fibroblast and osteosarcoma cell lines, ANRIL levels rise in response to a variety of DNA damaging agents via an ATM-induced E2F1 transcription-mediated pathway (29) and ANRIL contributes to pro-inflammatory cytokine expression by binding to YingYang-1 (YY1) in response to NFκB signaling (8). POSTN is regulated by YY1, so it is possible that periostin may be an additional cytokine to IL6/8 regulated by the YY1/ ANRIL complex (30). Considering periostin is commonly produced by stromal cells the relevance of the contribution from malignant cells is unclear.…”

Section: Discussionmentioning

confidence: 99%

Long Non-coding RNA ANRIL in the Nucleus Associates With Periostin Expression in Breast Cancer

et al. 2019

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The long non-coding RNA (LncRNA) antisense RNA in the INK4 locus (ANRIL) is overexpressed in several cancers including breast cancer. To better understand the role of ANRIL in breast cancer this study investigated where ANRIL was expressed in breast tumors using in situ hybridization by RNAscope. Additional RNAscope assays for IL6, CCL2, and POSTN were used to establish whether ANRIL correlated with increased tumor promoting cytokines. Breast tumors with ANRIL over expressed from real-time quantitative (RT-q) PCR assays were selected for analysis using RNAscope. All tumors showed ANRIL expression in malignant cells, but amongst tumors ANRIL showed different subcellular locations with 56% of tumors with ANRIL only in the nucleus, 16% with ANRIL only in the cytoplasm and 28% with ANRIL in both the nucleus and cytoplasm. Cases with nuclear ANRIL were positively correlated with POSTN expression in malignant cells (ρ = 0.57, P = 0.0086), and no correlation was found between ANRIL and IL6 or CCL2. Reduced POSTN was also found using siRNA to ANRIL in MDA-MB-231 and MCF7 breast cancer cells. These data indicate that ANRIL is expressed in malignant breast cells, and suggest its subcellular location may indicate its function in cancer progression.

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Negative transcriptional regulation of the human periostin gene by YingYang-1 transcription factor

Cited by 14 publications

References 35 publications

Clustering of patients with intrahepatic cholangiocarcinoma based on serum periostin may be predictive of prognosis

Clustering of patients with intrahepatic cholangiocarcinoma based on serum periostin may be predictive of prognosis

Hepatitis B virus replication and sex-determining region Y box 4 production are tightly controlled by a novel positive feedback mechanism

Long Non-coding RNA ANRIL in the Nucleus Associates With Periostin Expression in Breast Cancer

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