NEK2 phosphorylation antagonizes the microtubule stabilizing activity of centrobin

Park, Joonhyun; Rhee, Kunsoo

doi:10.1016/j.bbrc.2012.12.106

Cited by 20 publications

(28 citation statements)

References 25 publications

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“…The disassembled linker proteins facilitate centrosome separation and the establishment of the mitotic spindle (35,(37)(38)(39)(40)(41). In addition to the role in centrosome separation, NEK2 is also known to be involved in meiotic events, including chromosomal condensation and segregation (42,43), in microtubule stabilization through NIP2/centrobin (44,45), and in kin-etochore microtubule attachment stability through Hec1 phosphorylation (46 -48).…”

mentioning

confidence: 99%

Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) Protein Is Involved in Centrosome Separation through the Regulation of NIMA (Never In Mitosis Gene A)-related Kinase 2 (NEK2) Protein Activity

Jeong

Lee

Park

et al. 2014

Journal of Biological Chemistry

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Background: Cancerous inhibitor of protein phosphatase 2A (CIP2A) is overexpressed in most types of human cancer. Results: Depletion of CIP2A prolongs cell division time and CIP2A interacts with NIMA-related kinase 2 (NEK2) during G 2 /M phase to facilitate centrosome separation. Conclusion: CIP2A is involved in cell cycle progression through centrosome separation and mitotic spindle dynamics.Significance: This provides a novel role for CIP2A in cell cycle progression.

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mentioning

confidence: 99%

Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) Protein Is Involved in Centrosome Separation through the Regulation of NIMA (Never In Mitosis Gene A)-related Kinase 2 (NEK2) Protein Activity

Jeong

Lee

Park

et al. 2014

Journal of Biological Chemistry

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“…8,10 NEK2 phosphorylation reduces the microtubule nucleation activity of centrobin while PLK1 phosphorylation enhances it. 8,10 In fact, we observed that the phospho-resistant mutant centrobin against NEK2 enhanced the microtubule formation in both centrosome and cytoplasm, implying that centrobin mediates NEK2 regulation on microtubule formation (Fig. 5C).…”

Section: Discussionmentioning

confidence: 79%

“…10 The phospho-resistant mutant of centrobin at these residues (CBN4A) enhanced microtubule stabilizing activity, suggesting that NEK2 antagonizes the centrobin activity by phosphorylation. 10 First, we depleted endogenous NEK2 of U2OS cells with siRNA transfection and performed microtubule regrowth assays (Fig. 4A).…”

Section: Resultsmentioning

confidence: 99%

“…PLK1 enhances the microtubule stabilizing activity of centrobin during mitosis whereas NEK2 antagonizes it in interphase cells. 8,10 The microtubule organizing activity of centrobin is critical for asymmetric division of Drosophila neuroblasts. 11 Centrobin is necessary and sufficient for daughter centriole anchoring at the apical region of the neuroblasts.…”

Section: Introductionmentioning

confidence: 99%

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The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm

Shin

Kaang

et al. 2015

Cell Cycle

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Centrobin resides in daughter centriole and play a critical role in centriole duplication. Nucleation and stabilization of microtubules are known biological activities of centrobin. Here, we report a specific localization of centrobin outside the centrosome. Centrobin was associated with the stable microtubules. In hippocampal cells, centrobin formed cytoplasmic dots in addition to the localization at both centrosomes with the mother and daughter centrioles. Such specific localization pattern suggests that cytoplasmic centrobin is not just a reserved pool for centrosomal localization but also has a specific role in the cytoplasm. In fact, centrobin enhanced microtubule formation outside as well as inside the centrosome. These results propose specific roles of the cytoplasmic centrobin for noncentrosomal microtubule formation in specific cell types and during the cell cycle.

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“…The microtubule‐stabilizing activity of centrobin is dependent on its phosphorylation state . PLK1‐mediated phosphorylation positively regulates the microtubule stabilizing ability of centrobin while never in mitosis gene A‐related kinase (NEK2)‐mediated phosphorylation of centrobin negatively regulates the microtubule‐stabilizing activity of centrobin . Centrobin also regulates the amount of PCM in the centrosome but the mechanism of stabilization of microtubules still remains elusive.…”

Section: Centrosomal Proteins As Microtubule Stabilizersmentioning

confidence: 99%

Regulation of microtubule stability by centrosomal proteins

Kumari

Panda

2018

IUBMB Life

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The centrosome executes diverse functions including the nucleation and organization of microtubules. A subset of centrosomal proteins is found to be involved in regulating the nucleation, stability, and dynamics of microtubules. Literature is flooded with reports of centrosomal proteins regulating microtubule nucleation. However, the centrosomal proteins that regulate microtubule stability are underexplored. Here, we review the centrosomal proteins, which either enhance or reduce the stability of microtubules and thereby regulate microtubule dynamics. We also discuss unexplored aspects of the centrosomal proteins that influence microtubule stability. © 2018 IUBMB Life, 70(7):602-611, 2018.

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NEK2 phosphorylation antagonizes the microtubule stabilizing activity of centrobin

Cited by 20 publications

References 25 publications

Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) Protein Is Involved in Centrosome Separation through the Regulation of NIMA (Never In Mitosis Gene A)-related Kinase 2 (NEK2) Protein Activity

Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) Protein Is Involved in Centrosome Separation through the Regulation of NIMA (Never In Mitosis Gene A)-related Kinase 2 (NEK2) Protein Activity

The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm

Regulation of microtubule stability by centrosomal proteins

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