Nematic growth of microtubules that changed into giant spiral structure through partial depolymerization and subsequent dynamic ordering

Shigehara, Kiyotaka; Kudoh, Hideyuki; Mori, Shiori; Tamura, Yoshiki; Kakugo, Akira; Kawamura, Ryuzo; Furukawa, Hidemitsu; Gong, Jian Ping; Masunaga, Hiroyasu; Masui, Tomomi; Koizumi, Satoshi; Shikinaka, Kazuhiro

doi:10.1039/c2sm26675j

Cited by 11 publications

(21 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[110][111][112][113][114][115][116][117][118][119][120][121][122][123][124][125][126] For example, the chiral nematic liquid crystal phase of a 1D rod-like virus can act as a template to enable its self-assembly in the presence of monomers, crosslinkers, and initiators. [112] After polymerization and etching away the virus templates, aligned hydrogels remain.…”

Section: Self-assemblymentioning

confidence: 99%

“…[120] Meanwhile, many researchers have studied creating highly ordered structures of biomolecules by using a self-assembly strategy. [121][122][123] For example, tubulin, as a cytoskeletal protein, can form a cylindrical assembly called a microtubule. In artificial systems, well-ordered alignment of microtubules was obtained by performing asymmetric polymerization in a confined space.…”

Section: Self-assemblymentioning

confidence: 99%

See 1 more Smart Citation

Bioinspired Nanocomposite Hydrogels with Highly Ordered Structures

et al. 2017

View full text Add to dashboard Cite

In the human body, many soft tissues with hierarchically ordered composite structures, such as cartilage, skeletal muscle, the corneas, and blood vessels, exhibit highly anisotropic mechanical strength and functionality to adapt to complex environments. In artificial soft materials, hydrogels are analogous to these biological soft tissues due to their “soft and wet” properties, their biocompatibility, and their elastic performance. However, conventional hydrogel materials with unordered homogeneous structures inevitably lack high mechanical properties and anisotropic functional performances; thus, their further application is limited. Inspired by biological soft tissues with well‐ordered structures, researchers have increasingly investigated highly ordered nanocomposite hydrogels as functional biological engineering soft materials with unique mechanical, optical, and biological properties. These hydrogels incorporate long‐range ordered nanocomposite structures within hydrogel network matrixes. Here, the critical design criteria and the state‐of‐the‐art fabrication strategies of nanocomposite hydrogels with highly ordered structures are systemically reviewed. Then, recent progress in applications in the fields of soft actuators, tissue engineering, and sensors is highlighted. The future development and prospective application of highly ordered nanocomposite hydrogels are also discussed.

show abstract

Section: Self-assemblymentioning

confidence: 99%

Section: Self-assemblymentioning

confidence: 99%

Bioinspired Nanocomposite Hydrogels with Highly Ordered Structures

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Two dimensional-scattering pattern and profile of spherulite appeared from evaporating droplet containing 180 μM tubulin solution were shown in Fig 4B. The 2D diffraction pattern of the radially aligned MTs after heating shows the tilt angle from its equator position indicating long-range ordering of MTs which is quite different from the isotropic orientation of MTs in solution (Fig 4B (i), (ii)). Sharp peaks arising from the near-meridional reflections with d value of 2.23, 1.78, 1.58 and 1.12 nm (Fig 4C (ii); 1, 2, 3, 4 respectively) might be due to ordering of MTs in radially aligned pattern in evaporated state which differs from the wider peaks of MTs in solution [38] (Fig 4C (i); 1, 2, 3, 4 respectively). The higher-ordered alignments may give rise to these sharp peaks in the layer lines of detected d-spacing values which are similar to the previously reported values [39].…”

Section: Plos Onementioning

confidence: 98%

Radial alignment of microtubules through tubulin polymerization in an evaporating droplet

Keya¹,

Kudoh²,

Kabir³

et al. 2020

PLoS ONE

Self Cite

View full text Add to dashboard Cite

We report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.

show abstract

“…50,52 The direct electrostatic interaction between MTs, which has been theoretically estimated treating MTs as ordered electrets, 26 may be relevant as it may support the long range spiral order experimentally observed in a MT-crowded system. 55 Additionally, local electric fields produced by micro/nano-wire electrodes may work as external control parameters in the study of phase transitions. 56…”

Section: Electric Field-induced Mt Condensationmentioning

confidence: 99%

Electric field-induced reversible trapping of microtubules along metallic glass microwire electrodes

Kim

Sikora

Nakayama

et al. 2015

Journal of Applied Physics

View full text Add to dashboard Cite

Microtubules are among bio-polymers providing vital functions in dynamic cellular processes. Artificial organization of these bio-polymers is a requirement for transferring their native functions into device applications. Using electrophoresis, we achieve an accumulation of microtubules along a metallic glass (Pd 42.5 Cu 30 Ni 7.5 P 20) microwire in solution. According to an estimate based on migration velocities of microtubules approaching the wire, the electrophoretic mobility of microtubules is around 10 À12 m 2 /Vs. This value is four orders of magnitude smaller than the typical mobility reported previously. Fluorescence microscopy at the individual-microtubule level shows microtubules aligning along the wire axis during the electric field-induced migration. Caseintreated electrodes are effective to reversibly release trapped microtubules upon removal of the external field. An additional result is the condensation of secondary filamentous structures from oriented microtubules. V

show abstract

Nematic growth of microtubules that changed into giant spiral structure through partial depolymerization and subsequent dynamic ordering

Cited by 11 publications

References 27 publications

Bioinspired Nanocomposite Hydrogels with Highly Ordered Structures

Bioinspired Nanocomposite Hydrogels with Highly Ordered Structures

Radial alignment of microtubules through tubulin polymerization in an evaporating droplet

Electric field-induced reversible trapping of microtubules along metallic glass microwire electrodes

Contact Info

Product

Resources

About