Nematicidal activity of 5-iodoindole against root-knot nematodes

Rajasekharan, Satish Kumar; Kim, Seulbi; Kim, Jin-Cheol; Lee, Jintae

doi:10.1016/j.pestbp.2019.10.012

Cited by 40 publications

(46 citation statements)

References 22 publications

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“…Of the halogenated indoles tested, 5-iodoindole and 6-iodoindole most effectively controlled A. baumannii cell growth and biofilm formation ( Figure S1). Previously, 5-iodoindole was reported to be nontoxic in a mouse model and in plant germination models [24][25][26], but to rapidly kill pine wood [25] and root-knot nematodes [26], which in combination with our findings suggests 5-iodoindole is relatively safe for biological applications.…”

Section: Discussionsupporting

confidence: 78%

“…In the same study, it was hypothesized that these derivatives bound in the interdomain cleft of FtsZ, interrupted GTPase activity, stimulated FtsZ polymerization, and ultimately inhibiting bacterial cell division [55]. Since 5-iodoindole decreased cell size of E. coli strains and C. albicans (Figure 8) and was found to rapidly kill A. baumannii (Figure 4), other microbes (Figure 7), three nematode species [24][25][26][27], and an insect [25], but not plants or vertebrates [25,26], it would appear that 5-iodoindole is selectively transported through the membranes of bacteria, fungi, and nematodes and then specifically targets DNA.…”

Section: Discussionmentioning

confidence: 98%

“…Among a series of halogenated indoles, 5-iodoindole eradicated E. coli and S. aureus persister cells and biofilms via an unknown mechanism [ 22 ] and killed the bacterivorous nematode Caenorhabditis elegans [ 23 ], pinewood nematode Bursaphelenchus xylophilus [ 24 , 25 ], and root-knot nematode Meloidogyne incognita [ 26 ], via methuosis [ 27 ]. Furthermore, 5-iodoindole has been shown to be nontoxic to plants and animals [ 25 , 26 ]. However, the mechanism responsible for the effects of 5-iodoindole in bacteria has not been determined and its efficacy has not been studied against MDR A .…”

Section: Introductionmentioning

confidence: 99%

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Rapid Killing and Biofilm Inhibition of Multidrug-Resistant Acinetobacter baumannii Strains and Other Microbes by Iodoindoles

Raorane

Lee

2020

Biomolecules

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Multi-drug resistant Acinetobacter baumannii is well-known for its rapid acclimatization in hospital environments. The ability of the bacterium to endure desiccation and starvation on dry surfaces for up to a month results in outbreaks of health care-associated infections. Previously, indole and its derivatives were shown to inhibit other persistent bacteria. We found that among 16 halogenated indoles, 5-iodoindole swiftly inhibited A. baumannii growth, constrained biofilm formation and motility, and killed the bacterium as effectively as commercial antibiotics such as ciprofloxacin, colistin, and gentamicin. 5-Iodoindole treatment was found to induce reactive oxygen species, resulting in loss of plasma membrane integrity and cell shrinkage. In addition, 5-iodoindole rapidly killed three Escherichia coli strains, Staphylococcus aureus, and the fungus Candidaalbicans, but did not inhibit the growth of Pseudomonas aeruginosa. This study indicates the mechanism responsible for the activities of 5-iodoindole warrants additional study to further characterize its bactericidal effects on antibiotic-resistant A. baumannii and other microbes.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Rapid Killing and Biofilm Inhibition of Multidrug-Resistant Acinetobacter baumannii Strains and Other Microbes by Iodoindoles

Raorane

Lee

2020

Biomolecules

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“…In the current study, the effect of pure volatiles, i.e., methyl isovalerate (MIV) and 2-undecanone (2-UD), with strong nematicidal activity was studied for plant growth promotion and induced systemic resistance against M. incognita at a broader level in tomato plants in an experiment in greenhouse designed according to the previous protocol with some modifications [ 14 , 38 , 39 ]. Four-leaf stage tomato seedlings of equal size were transplanted into each pot attached to a jar having pure methyl isovalerate (MIV) and 2-undecanone (2-UD) at different concentrations up to 150 µg/mL.…”

Section: Resultsmentioning

confidence: 99%

Nematicidal Volatiles from Bacillus atrophaeus GBSC56 Promote Growth and Stimulate Induced Systemic Resistance in Tomato against Meloidogyne incognita

Ayaz

Ali

Farzand

et al. 2021

IJMS

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Bacillus volatiles to control plant nematodes is a topic of great interest among researchers due to its safe and environmentally friendly nature. Bacillus strain GBSC56 isolated from the Tibet region of China showed high nematicidal activity against M. incognita, with 90% mortality as compared with control in a partition plate experiment. Pure volatiles produced by GBSC56 were identified through gas chromatography and mass spectrometry (GC-MS). Among 10 volatile organic compounds (VOCs), 3 volatiles, i.e., dimethyl disulfide (DMDS), methyl isovalerate (MIV), and 2-undecanone (2-UD) showed strong nematicidal activity with a mortality rate of 87%, 83%, and 80%, respectively, against M. incognita. The VOCs induced severe oxidative stress in nematodes, which caused rapid death. Moreover, in the presence of volatiles, the activity of antioxidant enzymes, i.e., SOD, CAT, POD, and APX, was observed to be enhanced in M. incognita-infested roots, which might reduce the adverse effect of oxidative stress-induced after infection. Moreover, genes responsible for plant growth promotion SlCKX1, SlIAA1, and Exp18 showed an upsurge in expression, while AC01 was downregulated in infested plants. Furthermore, the defense-related genes (PR1, PR5, and SlLOX1) in infested tomato plants were upregulated after treatment with MIV and 2-UD. These findings suggest that GBSC56 possesses excellent biocontrol potential against M. incognita. Furthermore, the study provides new insight into the mechanism by which GBSC56 nematicidal volatiles regulate antioxidant enzymes, the key genes involved in plant growth promotion, and the defense mechanism M. incognita-infested tomato plants use to efficiently manage root-knot disease.

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“…Several studies have described the antibiofilm and antivirulence activities of indole derivatives (e.g., 7-hydroxyindole, 3-indoleacetonitrile, 7-fluoroindole, 7benzyloxyindole, and methylindoles) against clinically important pathogens, such as enterohemorrhagic Escherichia coli (Lee et al, 2007), Pseudomonas aeruginosa (Lee et al, 2009(Lee et al, , 2011(Lee et al, , 2012, Staphylococcus aureus (Lee et al, 2013), and Candida albicans (Lee et al, 2018;Manoharan et al, 2018). In addition, indole and 3-indolylacetonitrile have been shown to inhibit the maturation of Paenibacillus alvei endospores (Kim Y.-G.et al, 2011), and halogenated indoles have been reported to have nematicidal and insecticidal potentials (Rajasekharan et al, 2019(Rajasekharan et al, , 2020. Although the QS inhibitory activity of indole in S. marcescens has been clearly reported in previous work (Hidalgo-Romano et al, 2014), the effects of indole derivatives on the biofilm and virulence of indole-negative S. marcescens have yet to be evaluated, and thus in the present study, we investigated the antibiofilm and antivirulence potentials of several indole derivatives against S. marcescens.…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and Antivirulence Properties of Indoles Against Serratia marcescens

et al. 2020

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Indole and its derivatives have been shown to interfere with the quorum sensing (QS) systems of a wide range of bacterial pathogens. While indole has been previously shown to inhibit QS in Serratia marcescens, the effects of various indole derivatives on QS, biofilm formation, and virulence of S. marcescens remain unexplored. Hence, in the present study, we investigated the effects of 51 indole derivatives on S. marcescens biofilm formation, QS, and virulence factor production. The results obtained revealed that several indole derivatives (3-indoleacetonitrile, 5-fluoroindole, 6-fluoroindole, 7-fluoroindole, 7-methylindole, 7-nitroindole, 5-iodoindole, 5-fluoro-2methylindole, 2-methylindole-3-carboxaldehyde, and 5-methylindole) dose-dependently interfered with quorum sensing (QS) and suppressed prodigiosin production, biofilm formation, swimming motility, and swarming motility. Further assays showed 6fluoroindole and 7-methylindole suppressed fimbria-mediated yeast agglutination, extracellular polymeric substance production, and secretions of virulence factors (e.g., proteases and lipases). QS assays on Chromobacterium violaceum CV026 confirmed that indole derivatives interfered with QS. The current results demonstrate the antibiofilm and antivirulence properties of indole derivatives and their potentials in applications targeting S. marcescens virulence.

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Nematicidal activity of 5-iodoindole against root-knot nematodes

Cited by 40 publications

References 22 publications

Rapid Killing and Biofilm Inhibition of Multidrug-Resistant Acinetobacter baumannii Strains and Other Microbes by Iodoindoles

Rapid Killing and Biofilm Inhibition of Multidrug-Resistant Acinetobacter baumannii Strains and Other Microbes by Iodoindoles

Nematicidal Volatiles from Bacillus atrophaeus GBSC56 Promote Growth and Stimulate Induced Systemic Resistance in Tomato against Meloidogyne incognita

Antibiofilm and Antivirulence Properties of Indoles Against Serratia marcescens

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