Nematicidal activity of some strains of Pseudomonas spp.

Ali, Nasima Imam; Siddiqui, I. A.; Shaukat, Syed Shahid; Zaki, M. J.

doi:10.1016/s0038-0717(02)00029-9

Cited by 91 publications

(48 citation statements)

References 23 publications

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“…The P. putida UW4 strain was tested for nematicidal activity against B. xylophilus by the methods described by Samaliev et al (2000) and Ali et al (2002). Bursaphelenchus xylophilus ''HF'' culture was used for the nematicidal assays.…”

Section: Nematicidal Activity Assaymentioning

confidence: 99%

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Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

et al. 2012

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Pine wilt disease, caused by the nematode Bursaphelenchus xylophilus, is responsible for devastation of pine forests worldwide. Until now, there are no effective ways of dealing with this serious threat. The use of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (encoded by the acdS gene)-producing plant growth-promoting bacteria has been shown to be a useful strategy to reduce the damage due to biotic and abiotic stresses. Pinus pinaster seedlings inoculated with the ACC deaminase-producing bacterium Pseudomonas putida strain UW4 showed an increased root and shoot development and reduction of B. xylophilus induced symptoms. In contrast, a P. putida UW4 acdS mutant was unable to promote pine seedling growth or to decrease B. xylophilus induced symptoms. This is the first report on the use of ACC deaminase-producing bacteria as a potential biological control agent for a tree disease, thus suggesting that the inoculation of pine seedlings grown in a tree nursery might constitute a novel strategy to obtain B. xylophilus resistant pine trees.

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Section: Nematicidal Activity Assaymentioning

confidence: 99%

“…The supernatant was collected and filtered using a 0.22 lm filter in order to obtain a cell-free culture filtrate (Ali et al 2002).…”

Section: Nematicidal Activity Assaymentioning

confidence: 99%

Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

et al. 2012

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“…This result indicates that nematicidal activity seems to be widespread among ERFP isolates. In the literature, the proportions of rhizosphere bacteria with nematicidal activity was lower than that found in the present study and were 58-74 % [1], 70 % [33], 65 % [34] Based on the screening results, the five isolates which showed the highest nematicidal activity (Ps 54, Ps 36, Ps 22, Ps 21 and Ps 14) were selected for further investigation. In the in vitro experiment, cultures and cell-free supernatants of the five isolates showed a significant reduction in the number of eggs hatching and a significant increase in mortality of J 2 of M. incognita.…”

Section: Discussionmentioning

confidence: 92%

“…Strains of both species are commonly encountered in the plant rhizosphere and many of these strains have been frequently reported as PGPR and as biological control agents [44]. It is worth mentioning that Ali et al [33] pointed to some health risks may be involved in the application of P. aeruginosa to control plant pathogens. In the light of data available about the distribution of the P. aeruginosa in plant rhizospheres and their consideration by a number of researches as plant growth promoters and biocontrol agents against a lot of plant pathogens, the question arises if these strains are human pathogens or if they are completely different from human pathogenic strains.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Gamal¹

2010

J Biofertil Biopestici

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A total of 52 Egyptian rhizosphere fluorescent pseudomonad isolates (ERFP) were screened for their nematicidal activity in vitro. The screening results showed an inhibitory effect on hatching of Meloidogyne incognita eggs ranging from 57 % to 100 %. Similar to the chemical nematicide Videt in vitro experiment, cultures or cell-free supernatants (CFS) of the ERFP isolates showed complete inhibition of egg hatching and killed 100 % of the J 2 . In glasshouse pot experiments, cultures of these isolates, CFS and the chemical nematicide showed reduction in root galling ranging from 81. 0-95.4, 61.3-84.5, 97.3%, respectively. Also, the reduction in nematode multiplication in soil was 91. 9-95.7, 83.5-84.5 and 96.5% for cultures, CFS and the chemical nematicide, respectively, compared to the positive control (nematode only). Furthermore, these isolates showed significant increases in plant growth characters, phenolic content and activities of the plant defense-related enzymes. Based on 16S rDNA sequences and API NE kits, two major clusters were observed; strains Ps 36 and Ps 54 appeared to fall within the variability range of P. putida, while Ps 21, Ps 22 and Ps 14 are likely to be strains of P. aeruginosa. The distinctness of Ps 36 and Ps 54 from Ps 14, Ps 21 and Ps 22 was supported by their physiological characteristics, such as the ability of Ps 14 and Ps 21 and Ps 22 to utilize mannitol, N-acetyl-glucose amine and adipate. Johnsen et al. [14]. DNA 16S region amplification was performed using the primer set 16SF-16SR [15]. The 16S rDNA was sequenced as described by Sanger et al. [16]. The 16S rDNA sequences were initially analyzed by using the program Blast (National Center Biotechnology Information, http//:www.ncbi.nml.nih.gov). Sequencing data obtained from different primers were collected together using the CAP program (Contig Assembly and Genomic Expression programs). The consensus sequence from the isolates and sequences of strains belonging to the same phylogenetic group and of other representatives of Pseudomonas strains (retrieved from the NCBI database) were aligned using the computer-program ClustalX [17]. The resulting trees were displayed with Tree View [18]. The phylogenetic tree was calculated using the neighbor-joining method [19] and Acinetobacter calcoaceticus and E. coli K12 were used as outgroups. Preparation of bacterial inocula and their cell -free culture supernatants (CFS)The pseudomonad isolates were grown separately for 36 h in King's B medium broth at 28°C on a rotary shaker at 150 rpm. Part of the cultures was kept without centrifugation for use at 4°C. The bacterial cultures mixture was prepared by mixing equal volumes of cell suspension of individual isolates. For preparation of cell-free culture filtrate, the other part of the bacterial cultures were centrifuged at 5,000 x g for 30 min; pellets were discarded and the supernatants were filtered through bacterial filter (0.22μm pore size) and kept for use at 4°C. Preparation of nematode inoculaA population of the root-knot nemat...

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“…Nematicidal of hydrogen cyanide produced by P. fluorescens CHAO were reported [11 & 13]. Protease and glycoprotease production by P. aeruginosa showed nematididal activity [1]. Bacterial strains isolated from olive rhizosphere soil were more effective than those isolated from another source such as P. fluorescens CHAO under greenhouse condition.…”

Section: Discussionmentioning

confidence: 99%

Diminish of Olive Root Knot Nematode Population and Promoting of Host Growth by Fluorescent Pseudomonads

2017

Dec. 25-26, 2017 Bangkok (Thailand) IETCAS-17, EABNS-2017, DBMCE-17, AHSCSR-17 &Amp; LEBL-17

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Nematicidal activity of some strains of Pseudomonas spp.

Cited by 91 publications

References 23 publications

Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Diminish of Olive Root Knot Nematode Population and Promoting of Host Growth by Fluorescent Pseudomonads

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