Nematicidal activity of teleocidin B4 isolated from Streptomyces sp. against pine wood nematode, Bursaphelenchus xylophilus

Abstract

BACKGROUND Pine wilt disease (PWD) is a plant disease that causes serious damage to pine trees. PWD occurs when the host plant is infected with pinewood nematode (PWN), Bursaphelenchus xylophilus. In this study, a compound with nematicidal activity was isolated from actinomycetes and its efficacy was investigated in vitro. RESULT We screened and selected Streptomyces sp. 680560, which had nematicidal activity against B. xylophilus. Based on 16S rRNA sequence analysis, it showed 99.93% similarity with Streptomyces blastmyceticus NRRB‐5480T. Furthermore, the active compound was isolated and identified as teleocidin B4. Teleocidin B4 at concentrations ranging from 6.25 to 100 μM had low nematicidal activity after 24 and 36 h against adult and stage juveniles (J2) of B. xylophilus, but after 48 h nematicidal activity exceeded 95%. The rate of inhibition of egg hatching for Teleocidin B4 6.25, 12.5, 25, 50, and 100 μM was confirmed to be dose‐dependently inhibited after 48 h of treatment. Teleocidin B4 is not only toxic to hatched B. xylophilus, but also affects egg hatching. CONCLUSION This study was carried out to isolate actinomycete metabolites from pine tree endophytes from various natural environments for control of PWD. A compound with nematicidal activity was isolated from a selected strain and its structure was identified as teleocidin B4. The nematicidal effect of the isolated active substance, teleocidin B4, was confirmed. This is the first report of the effect of teleocidin B4 on B. xylophilus, suggesting its possibility as a PWD control agent. © 2020 Society of Chemical Industry

Actinomycins produced by endophyte Streptomyces sp. GLL‐9 from navel orange plant exhibit high antimicrobial effect against Xanthomonas citri susp. citri and Penicillium italicum

“…GLL‐9 had excellent inhibitory effects against both Xcc and P. italicum , while its ethyl acetate extract (EAE) had an even greater inhibitory effect with MIC values of 31.25 ( P. italicum ) and 62.50 (Xcc) μg mL −1 . According to previous reports, the secondary metabolites from Streptomyces species had inhibitory effects on several plant pathogens and could act as biological control agents against many agricultural pests 47,48 . It was reported that the primary microorganism responsible for the production of antibiotics is Streptomyces 49 .…”

“…According to previous reports, the secondary metabolites from Streptomyces species had inhibitory effects on several plant pathogens and could act as biological control agents against many agricultural pests. 47,48 It was reported that the primary microorganism responsible for the production of antibiotics is Streptomyces. 49 The Streptomyces species was widely used for the production of some antibiotics such as rapamycin 50 and avermectin, 51 and both of them were effective in controlling pests and diseases in agricultural crops.…”

Natural nematicidal metabolites and advances in their biocontrol capacity on plant parasitic nematodes

“…Compound 240 was toxic toward B. xylophilus and affected egg hatching. 100 Chaetoglobosin A (ChA) 241 was isolated from Chaetomium globosum NK102; it exhibited adverse effects on the vitality of M. incognita , and significantly inhibited J2 penetration. 101 Compound 241 and chaetoglobosin B 242 were obtained from the endophytic strain C. globosum YSC5; both caused mortality of the J2 of M. javanica , with LC 50 values of 88.4 and 107.7 μg mL −1 after 72 h, respectively.…”

Design, Synthesis, Nematicidal, and Fungicidal Activities of Novel Azo and Azoxy Compounds

“…14,19,20 Natural products have been an important source for the development of new drugs and agrochemicals not only because of their diverse structures and biological activities but also because of their multiple modes of action and environmentally friendly characteristics. 7,21 Nowadays, usually avermectin and its derivative emamectin benzoate have been used for the prevention and treatment of B. xylophilus and Meloidogyne. 22−24 Jietacins (Figure 1) are a kind of natural product that were isolated from the fermentation broth of Streptomyces sp.…”