Nematicidal activity of volatile organic compounds emitted by Brassica juncea, Azadirachta indica, Canavalia ensiformis, Mucuna pruriens and Cajanus cajan against Meloidogyne incognita

Sci. agric. (Piracicaba, Braz.)

et al. 2017

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Since volatiles do not leave residues in food and cause low toxicity to humans, they can act as a skeleton for new nematicidal molecules, once they have demonstrated activity against phytonematodes. The aim of this study was to evaluate the effect of volatile organic compounds (VOCs) released by wood-associated fungi on controling second-stage juveniles (J2) of Meloidogyne incognita. All 28 wood-associated fungi isolates produced VOCs which caused in excess of 76 % immobility in exposed J2 of M. incognita. The fungus isolate VOCs also caused significant mortality compared to control when J2 were exposed to them. After 6 h of exposure, the fungus species Epicoccum nigrum and Schizophyllum commune produced VOCs that immobilized exposed J2 compounds, in relation to the control. When J2 were inoculated into tomato under greenhouse conditions, the M. incognita infectivity and reproduction were reduced by exposure to the VOCs. Volatiles produced by most of the fungi isolates and analyzed by gas chromatograph coupled to a mass spectrometer (CG-MS), included alcohols, esters, terpenes, and ketones. Certain compounds appeared only in S. commune or in E. nigrum suggesting their involvement in the high level of damage caused to nematode reproduction on tomato. Volatiles emitted by wood fungi demonstrated another mode of action of these microorganisms in nature.

Section: Discussionmentioning

confidence: 50%

Section: Toxicity In Vitro Of Vocs Emitted Wood-associated Fungi To Jmentioning

confidence: 99%

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Sci. agric. (Piracicaba, Braz.)

et al. 2017

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Pest Management Science

“…Subsequently, the VOCs from both assays were extracted via SPME and identified by gas chromatography–mass spectrometry (GC‐MS). The parameters for SPME and GC‐MS were identical to those described in previous work from our research group …”

Section: Methodsmentioning

confidence: 99%

“…Plant residues are used to control PPNs, primarily through their incorporation into the soil . At the same time, the search for new compounds with nematicidal activity in plant residues has attracted the interest of several researchers …”

Section: Introductionmentioning

confidence: 99%

“…exposure of M. incognita second‐stage juveniles (J 2 ) to 3‐methylbutyl acetate at 500 μg mL 1 for 1 h caused mortality above 84% . In recent years, studies on the use of plant VOCs against PPNs have expanded . Biofumigation, consisting of the incorporation of plant residues that slowly release their bioactive compounds into the soil, has been successfully used to control PPNs since the 19th century .…”

Section: Introductionmentioning

confidence: 99%

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Volatile emissions of watercress (Nasturtium officinale) leaves and passion fruit (Passiflora edulis) seeds against Meloidogyne incognita

Silva

Campos

Barros

et al. 2019

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BACKGROUND Plants emit volatile organic compounds (VOCs) with several functions, including toxicity to plant‐parasitic nematodes (PPNs). However, the toxicity of VOCs from watercress leaves (Nasturtium officinale) and passion fruit seeds (Passiflora edulis) against PPNs has not yet been studied. RESULTS Biofumigation with watercress leaves and passion fruit seeds reduced the infectivity and reproduction of Meloidogyne incognita in tomato plants. The VOCs emitted by watercress leaves and passion fruit seeds caused immobility of M. incognita second‐stage juveniles (J2). The reduction in infectivity and reproduction of M. incognita reached 89% and 99%, respectively, when J2 were exposed to watercress VOCs. Additionally, water exposed to VOCs emitted by watercress caused 79% M. incognita J2 mortality. The volatilome of the toxic water contained 12 compounds, mainly alcohols. The emissions from watercress leaves and passion fruit seeds contained 26 and 12 compounds, respectively, according to gas chromatography–mass spectrometry analysis. The 1‐octanol occurring in watercress emissions demonstrated in vitro and in vivo nematicidal activity against M. incognita, with a lethal dose necessary to cause 50% mortality (LC50) of 382.5 μg mL−1. CONCLUSIONS Watercress leaf and passion fruit seed macerates emitted VOCs with nematicidal activity against M. incognita. The compound 1‐octanol identified in watercress emissions may be useful for the nematicide‐producing industry. © 2019 Society of Chemical Industry

Volatile organic compounds produced by castor bean cake incorporated into the soil exhibit toxic activity against Meloidogyne incognita

Campos

Pest Management Science

et al. 2018

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