Nematicidal and Antifungal Activities of Annonaceous Acetogenins from Annona squamosa against Various Plant Pathogens

Abstract

The methanol extract of Annona squamosa seeds was highly active against two phytoparasitic nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. It efficiently suppressed plant diseases, caused by Phytophthora infestans and Puccinia recondita. Ten annonaceous acetogenins (AAs) were isolated, and their chemical structures were identified by mass and nuclear magnetic resonance spectral data. Out of 10 substances, eight displayed strong in vitro nematicidal activity against B. xylophilus with LD(50) values ranging 0.006 to 0.048 μg/mL. Squamocin-G showed potent nematicidal activity against M. incognita. Squamocin, squamocin-G, and squamostatin-A also displayed potent in vitro and in vivo antifungal activities against P. infestans causing tomato late blight. In addition, squamostatin-A effectively controlled the development of wheat leaf rust caused by P. recondita. Our findings suggested that A. squamosa seeds and its bioactive AAs can be an alternative resource of a promising botanical nematicide and fungicide to control various plant diseases.

Pharmacological Activities of <i>Annona squamosa</i>: Updated Review

“…Organic and aqueous extracts of Annona squamosa processes antifungal activity against important fungal strains -Alternaria alternate, Candida albicans, Fusarium solani, Microsporum canis and Aspergillus niger [39]. The active anti-fungal constituents of the leaves are 16-hentriacontanone (palmitone) and 10-hydroxy-16-henriacontanone while squamocin A and G, and squamostatin A are known antifungal chemicals present in the seeds [64].…”

Synthesis, Biological Activity and Action Mechanism Study of Novel Chalcone Derivatives Containing Malonate

“…Plant diseases are usually caused by invasive plant pathogens and are widespread and toxic, seriously threatening agricultural output and the quality of global agricultural production; moreover, they have become one of the most important problems in agricultural production . One of the world famous rice pathogens, Xanthomonas oryzae pv.…”

Spatio‐Temporal Variation of Alkaloids in Annona purpurea and the Associated Influence on Their Antifungal Activity

“…[6,7] However, published studies have not considered that the biosyn-thesis of SMs can be altered by the abiotic conditions to which plants are exposed, and, therefore, the performance and activity of the compounds will be affected. These metabolites have potent antimicrobial, anticancer, antimalarial, neuropharmacological, and insecticidal activities.…”

“…These metabolites have potent antimicrobial, anticancer, antimalarial, neuropharmacological, and insecticidal activities. [6,7] However, published studies have not considered that the biosyn-thesis of SMs can be altered by the abiotic conditions to which plants are exposed, and, therefore, the performance and activity of the compounds will be affected.…”