Nematode and Arthropod Genomes Provide New Insights into the Evolution of Class 2 B1 GPCRs

Cardoso, João C. R.; Félix, Rute C.; Power, Deborah M.

doi:10.1371/journal.pone.0092220

Cited by 26 publications

(14 citation statements)

References 88 publications

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“…Both Bayesian Inference (BI) and Maximum Likelihood (ML) trees had similar branching topologies (Fig. 2, Supplementary Figure 1) and receptor clustering agreed with the previously proposed theories about family B GPCR evolution 43 . The distribution of the mollusc family B GPCRs confirmed that they possess members of the five vertebrate and invertebrate receptor subfamilies.…”

Section: Lophotrochozoan Family B Gpcr Phylogeny Phylogenetic Analyssupporting

confidence: 85%

“…Characterization of the lophotrochozoan family B GPCRs confirmed that members descended from a unique bilaterian ancestral gene by gene duplication prior to the protostome-deuterostome split 43 . In common with nematodes and arthropods, the clustering of the mollusc and other lophotrochozoan receptors suggests that they probably arose by lineage and species-specific gene duplications driven in part by organismal adaptation to the environment.…”

Section: Discussionmentioning

confidence: 82%

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The calcitonin-like system is an ancient regulatory system of biomineralization

Cardoso

Félix

Ferreira

et al. 2020

Sci Rep

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Biomineralization is the process by which living organisms acquired the capacity to accumulate minerals in tissues. Shells are the biomineralized exoskeleton of marine molluscs produced by the mantle but factors that regulate mantle shell building are still enigmatic. this study sought to identify candidate regulatory factors of molluscan shell mineralization and targeted family B G-protein coupled receptors (GpcRs) and ligands that include calcium regulatory factors in vertebrates, such as calcitonin (cALc). in molluscs, cALc receptor (cALcR) number was variable and arose through lineage and speciesspecific duplications. The Mediterranean mussel (Mytilus galloprovincialis) mantle transcriptome expresses six cALcR-like and two cALc-precursors encoding four putative mature peptides. Mussel cALcR-like are activated in vitro by vertebrate cALc but only receptor cALcRiic is activated by the mussel cALciia peptide (ec 50 = 2.6 ×10 −5 M). Ex-vivo incubations of mantle edge tissue and mantle cells with CALCIIa revealed they accumulated significantly more calcium than untreated tissue and cells. Mussel CALCIIa also significantly decreased mantle acid phosphatase activity, which is associated with shell remodelling. our data indicate the cALc-like system as candidate regulatory factors of shell mineralization. The identification of the CALC system from molluscs to vertebrates suggests it is an ancient and conserved calcium regulatory system of mineralization.

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Section: Lophotrochozoan Family B Gpcr Phylogeny Phylogenetic Analyssupporting

confidence: 85%

Section: Discussionmentioning

confidence: 82%

The calcitonin-like system is an ancient regulatory system of biomineralization

Cardoso

Félix

Ferreira

et al. 2020

Sci Rep

Self Cite

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“…Of the B-type GPCRs that have not been deorphanized in insects, there is one that appears to be the best candidate. This group of receptors is characterized by the B3 receptor from Bombyx (Yamanaka et al, 2008 ) and the receptor identified as 72 from Tribolium (Hauser et al, 2008 ) and corresponds to cluster A of Cardoso et al ( 2014 ). We thus looked for homologs of this receptor in other arthropod genomes and found such receptors only in those species that also have a calcitonin gene.…”

Section: Resultsmentioning

confidence: 99%

The contribution of the genomes of a termite and a locust to our understanding of insect neuropeptides and neurohormones

2014

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The genomes of the migratory locust Locusta migratoria and the termite Zootermopsis nevadensis were mined for the presence of genes encoding neuropeptides, neurohormones, and their G-protein coupled receptors (GPCRs). Both species have retained a larger number of neuropeptide and neuropeptide GPCRs than the better known holometabolous insect species, while other genes that in holometabolous species appear to have a single transcript produce two different precursors in the locust, the termite or both. Thus, the recently discovered CNMa neuropeptide gene has two transcripts predicted to produce two structurally different CNMa peptides in the termite, while the locust produces two different myosuppressin peptides in the same fashion. Both these species also have a calcitonin gene, which is different from the gene encoding the calcitonin-like insect diuretic hormone. This gene produces two types of calcitonins, calcitonins A and B. It is also present in Lepidoptera and Coleoptera and some Diptera, but absent from mosquitoes and Drosophila. However, in holometabolous insect species, only the B transcript is produced. Their putative receptors were also identified. In contrast, Locusta has a highly unusual gene that codes for a salivation stimulatory peptide. The Locusta genes for neuroparsin and vasopressin are particularly interesting. The neuroparsin gene produces five different transcripts, of which only one codes for the neurohormone identified from the corpora cardiaca. The other four transcripts code for neuroparsin-like proteins, which lack four amino acid residues, and that for that reason we called neoneuroparsins. The number of transcripts for the neoneuroparsins is about 200 times larger than the number of neuroparsin transcripts. The first exon and the putative promoter of the vasopressin genes, of which there are about seven copies in the genome, is very well-conserved, but the remainder of these genes is not. The relevance of these findings is discussed.

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“…6B), providing evidence for the expected parallel coevolution of ligandreceptor pairs. A recent evaluation of family B GPCRs in the genomes of various invertebrate species revealed putative receptors in insects and nematodes that align with a subgroup of human family B GPCRs comprised of the PTHR1 along with the receptors for secretin and glucagon; the findings would thus suggest that PTH receptors originated before the protostome-deuterostome divergence that is estimated to have occurred about 1000 million years ago (Cardoso et al, 2014).…”

Section: Evolution Of Parathyroid Hormone Receptors and Their Ligmentioning

confidence: 99%