Nematode Pheromones: Structures and Functions

Yang, Biyuan; Wang, Jie; Zheng, Xi; Wang, Xin

doi:10.3390/molecules28052409

Cited by 8 publications

(4 citation statements)

References 119 publications

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“…However, fungi become nematode predators by acquiring the conserved ascarosides released by nematodes. Many ascarosides have been identified as inducers of trap structures, such as Ascr#5, #7, and #18 [ 50 ]. The addition of Ascr#18 in this study did indeed promote the production of adhesive nets in A. oligospora ( Figure 1 B).…”

Section: Discussionmentioning

confidence: 99%

Ammonia and Nematode Ascaroside Are Synergistic in Trap Formation in Arthrobotrys oligospora

Huang,

Zheng,

Tian

et al. 2023

Pathogens

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Nematode-trapping (NT) fungi are natural predators of the soil living nematodes. Diverse external signals mediate the generation of predatory devices of NT fungi. Among these, broad ascarosides and nitrogenous ammonia are highly efficient inducers for trap structure initiation. However, the overlay effect of ammonia and ascaroside on the trap morphogenesis remains unclear. This study demonstrated that the combination of nitrogenous substances with nematode-derived ascarosides led to higher trap production compared to the single inducing cues; notably, ammonia and Ascr#18 had the most synergistic effect on the trap in A. oligospora. Further, the deletion of ammonia transceptor Amt43 blocked trap formation against ammonia addition in A. oligospora but not for the ascaroside Ascr#18 induction. Moreover, ammonia addition could promote plasma endocytosis in the process of trap formation. In contrast, ascaroside addition would facilitate the stability of intracellular organization away from endocytosis. Therefore, there is a synergistic effect on trap induction from different nitrogenous and ascaroside signals.

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Section: Discussionmentioning

confidence: 99%

Ammonia and Nematode Ascaroside Are Synergistic in Trap Formation in Arthrobotrys oligospora

Huang,

Zheng,

Tian

et al. 2023

Pathogens

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“…A similar effect of salinity on LLPS has been reported for polyarginine peptide series. 65 However, subtle changes are observed for longer oligolysines, such as K 24 and K 40 . Additionally, with the addition of ATP, the diffusion coefficient significantly decreases in systems capable of forming LLPS.…”

Section: Enthalpic Effectsmentioning

confidence: 99%

Understanding and fine tuning the propensity of ATP-driven liquid–liquid phase separation with oligolysine

Zhu,

Wu,

Luo

2024

Phys. Chem. Chem. Phys.

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“…A similar effect of salinity on LLPS has been reported for polyarginine peptide series. 64 However, subtle changes are observed for longer oligolysines, such as K 24 and K 40 . Additionally, with the addition of ATP, the diffusion coefficient significantly decreases in systems capable of forming LLPS.…”

Section: Entropic Effectsmentioning

confidence: 99%

Understanding and Fine Tuning the Propensity of ATP-Driven Liquid-Liquid Phase Separation with Oligolysine

Zhu,

Wu,

Luo

2024

Preprint

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Liquid-Liquid Phase Separation (LLPS) plays pivotal roles in the organization and functionality of living cells. It is imperative to understand the underlying driving forces behind LLPS and to control its occurrence. In this study, we employed coarse-grained (CG) simulations as a research tool to investigate systems comprising oligolysine and adenosine triphosphate (ATP) under conditions of various ionic concentrations and oligolysine lengths. Consistent with experimental observations, our CG simulations captured the formation of LLPS upon the addition of ATP and tendency of dissociating under high ionic concentration. The primary driving force behind this phenomenon is the electrostatic interaction between oligolysine and ATP. An in-depth analysis on the structural properties of LLPS was conducted, where the oligolysine structure remained unchanged with increased ionic concentration and the addition of ATP led to a more pronounced curvature, aligning with the observed enhancement of $\alpha$-helical secondary structure in experiments. In terms of the dynamic properties, the introduction of ATP led to a significant reduction in translational and vibrational degrees of freedom but not rotational degrees of freedom. Through keeping the total number of charged residues constant and varying their entropic effects, we constructed two systems of similar biochemical significance and further validated the entropy effects on the LLPS formation. These findings provide a deeper understanding of LLPS formation and shed lights on the development of novel bioreactor and primitive artificial cells for synthesizing key chemicals for certain diseases.

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Nematode Pheromones: Structures and Functions

Cited by 8 publications

References 119 publications

Ammonia and Nematode Ascaroside Are Synergistic in Trap Formation in Arthrobotrys oligospora

Ammonia and Nematode Ascaroside Are Synergistic in Trap Formation in Arthrobotrys oligospora

Understanding and fine tuning the propensity of ATP-driven liquid–liquid phase separation with oligolysine

Understanding and Fine Tuning the Propensity of ATP-Driven Liquid-Liquid Phase Separation with Oligolysine

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