Nematospiroides dubius in the mouse: evidence that adult worms depress the expression of homologous immunity

Behnke, Jerzy M.; Hannah, John; Pritchard, David

doi:10.1111/j.1365-3024.1983.tb00755.x

Cited by 90 publications

(37 citation statements)

References 28 publications

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“…Notably, the efficacy of this irradiated larval vaccine is diminished by the coadministration of unirradiated larvae, indicating that the development of adult worms is able to inhibit development and/or expression of protective immunity against subsequent reinfection [146, 147]. The ability of adult worms to suppress protective immunity was further demonstrated by vaccine failure in mice given irradiated larvae before or after receiving adult parasites by intraintestinal laparatomy [146] or oral gavage [149]. …”

Section: Vaccine-induced Immunitymentioning

confidence: 99%

Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus

2012

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Heligmosomoides polygyrus is a natural intestinal parasite of mice, which offers an excellent model of the immunology of gastrointestinal helminth infections of humans and livestock. It is able to establish long-term chronic infections in many strains of mice, exerting potent immunomodulatory effects that dampen both protective immunity and bystander reactions to allergens and autoantigens. Immunity to the parasite develops naturally in some mouse strains and can be induced in others through immunization; while the mechanisms of protective immunity are not yet fully defined, both antibodies and a host cellular component are required, with strongest evidence for a role of alternatively activated macrophages. We discuss the balance between resistance and susceptibility in this model system and highlight new themes in innate and adaptive immunity, immunomodulation, and regulation of responsiveness in helminth infection.

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Section: Vaccine-induced Immunitymentioning

confidence: 99%

Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus

2012

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“…It may also be that only certain stages of the life cycle of N. dubius can cause the changes in spleen which result in production of inactive CM; 6-day infections are larval, but by day 18 worms are adults. Adult worms are capable of depressing the ex pression of homologous immunity in mice [2] and may also be responsible for the changes reported here.…”

mentioning

confidence: 74%

“…N. dubius is known to exert immunosuppressive effects upon the host [2] and may also actively depress the potential for expression of an MMC response. Mice given primary infections of N. dubius fail to develop the intestinal mastocytosis which is characteristic of primary infections of N. brasiliensis or T. spiralis [3], Moreover, when infections of N. dubius are estab lished prior to or concurrently with infections of T. spiralis or N. brasiliensis, the MMC response elicited by these species is impaired as is expulsion of the worms [3].…”

mentioning

confidence: 99%

Medium Conditioned by Spleen Cells of <i>Nematospiroides dubiu</i><i>s</i>-lnfected Mice Does Not Support Development of Cultured Mast Cells

Reed¹,

Dehlawi²,

Wakelin³

1988

Int Arch Allergy Immunol

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The ability of different conditioned media to support mast cell development from precursors in normal bone marrow was evaluated. Many mast cells developed in bone marrow cultures containing medium conditioned by concanavalin-A-stimulated spleen cells of normal mice, Trichinella spiralis-infectedmice, or mice infected for 6 days by Nematospiroides dubius. By contrast, medium conditioned by concanavalin-A-stimulated spleen cells of mice having 18-day infections of the nematode N. dubius failed to support mast cell development. The difference between medium conditioned by spleen cells of mice having 6-day versus 18-day infections of N. dubius may be due to the life cycle stage of the parasite, larval or adult, present at those times. These results from cultures are consistent with those from in vivo studies in which mice given primary infections of N. dubius failed to develop the intestinal mastocytosis characteristic of nematode infections.

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“…The variety arises because of differences between quantity and quality among candidate vaccines and in the route of, and time between, vaccination and the challenge infection. Protective immunogens have been associated with both infective larvae (7,8) and soluble homogenates from adult N. dubius (9, 10).…”

Section: Introductionmentioning

confidence: 99%

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Monroy

Dobson

1987

Immunol Cell Biol

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Summary.A crude soluble extract (AWH) obtained from homogenized adult Nematospiraides dubius worms was fractionated into normal (NMtgAg) and immune (IMlgAg) antigens by sequential passage through Sepharose 4B, and CNBr-activaled Sepharose 4B to which immunoglobulins from normal (NMIg) and N. d»ft(U5-infected mouse serum (IMIg) had been coupled. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGF) under reducing condilions showed that MNtgAgand IMtgAg contained several proteins with molecular weights (MW) between 20,000 and 200,0(X). IMtgAg contained 2 polypeptides, MW 24,000 and 55,(XX), which reacted strongly in western bloi analysis against IMig; the larger molecule related lo a cuticular component of the worm. Protective high MW AWH G-200 filtrates shared 5 polypeptides with IMlgAg (MW 85,000-200.000) together with several smaller molecules (MW 30,000-< 10,000). The MW 55,O(X) and 24.0(X) components concentrated in JMlgAg were restricted to non-protective low MW AWH gel fiUraies. BCM mice vaccinated with IMtgAg were protected againsi N. dubius. Worm numbers, size and feciindily were reduced to an extent commensurate with that obtained in mice vaccinated wiih AWH. Mice vaccinaled wiih NMIgAg showed partial resistance. The role of surface and dominant parasite immunogens in immunity is discussed.

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Nematospiroides dubius in the mouse: evidence that adult worms depress the expression of homologous immunity

Cited by 90 publications

References 28 publications

Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus

Immunity to the model intestinal helminth parasite Heligmosomoides polygyrus

Medium Conditioned by Spleen Cells of <i>Nematospiroides dubiu</i><i>s</i>-lnfected Mice Does Not Support Development of Cultured Mast Cells

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

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