Nematospiroides dubius: response of the late fourth-stage larva to anthelmintics in vitro

Jenkins, D. C.; Ibarra, O. F.

doi:10.1007/bf00927827

Cited by 11 publications

(6 citation statements)

References 6 publications

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“…This may reflect relatively greater resistance to ivermectin by H. polygyrus, a possibility which is supported by the observation that in vitro cultured L4 stages of H. polygyrus responded to treatment with ivermectin in a distinctive way (Jenkins and Ibarra, 1984). The worms were not killed by relatively high doses of the drug although paralysis was induced at lower concentrations.…”

Section: Discussionmentioning

confidence: 88%

Factors affecting the efficacy of ivermectin against Heligmosomoides polygyrus (Nematospiroides dubius) in mice

Wahid

Behnke

Conway

1989

Veterinary Parasitology

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk Veterinary Parasitology, 32 (1989) The efficacy of ivermectin against Heligmosomoides polygyrus was investigated in 5 mouse strains (CFLP, NIH, C57Bllo, BALB/c and CBA ) using a variety of dose levels, and subcutaneous and oral administration, Heligmosomoides polygyrus were not completely eliminated when 5 mg kg 1 of ivermectin was given 6 days after infection, but 10 mg kg-~ was totally effective. Significant mouse-strain variation in drug efficacy was detected, NIH mice requiring treatment with higher doses than CFLP mice in order to bring about a comparable level of larvicidal activity. Ivermectin was more effective when given subcutaneously than when given orally, regardless of the dose administered or the strain of mouse tested. The anthelmintic effect of the treatment was more persistent in CFLP mice given 20 mg kg-~ subcutaneously than in NIH mice or in mice treated orally, and a 20-day interval between administration and infection was insufficient to prevent inhibition of parasite survival. Ivermectin was shown to be totally effective at 20 mg kg-1 given subcutaneously in killing immune arrested larvae of H. polygyrus.

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Section: Discussionmentioning

confidence: 88%

Factors affecting the efficacy of ivermectin against Heligmosomoides polygyrus (Nematospiroides dubius) in mice

Wahid

Behnke

Conway

1989

Veterinary Parasitology

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“…Studies in vitro on anthelmintic activity have been confined so far to fourth stage larvae (L4) or males and females incubated together. This has primarily involved Nematospiroides dubius (JENKINS & IBARRA, 1984),Nippostrongylus brasiliensis (JENKINS etal., 1980;JENKINS & CARRINGTON, 1982;RAPSON et al, 1986) and Trichostrongylus colubriformis (RAPSON etal., 1985;JENKINS etal., 1986).…”

Section: Discussionmentioning

confidence: 99%

“…A wide variety of nematodes maintained in vitro have been used for in vitro tests. Nematospiroides dubius (JENKINS & IBARRA, 1984), Nippostrongylus brasiliensis (JENKINS & CARRINGTON, 1982), and Trichostrongylus colubriformis (RAPSON etal., 1985) have been assayed using various methods to test their viability. Interpretation of anthelmintic activity in vitro largely relied on nematode development, viability and motility.…”

Section: Introductionmentioning

confidence: 99%

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

Mallet¹,

Kerbœuf²

1993

J. Helminthol.

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The inhibition of acetylcholinesterase secretion by male and female Heligmosomoides polygyrus was tested on worms taken from experimentally infected mice and maintained for 3 days in vitro in levamisole. The dose inhibiting 50% of enzyme secretion (ID 50) of male worms was twice the ID 50 for female worms. A similar difference was observed in vivo between the dose of levamisole removing 50% (LD 50) of male and female worms from the mouse. Acetylcholinesterase secretion by worms and ID 50 were tested in vitro at 3-weekly intervals from 3 to 21 weeks post infection (WPI). Acetylcholinesterase secretion was always significantly higher for male than for female worms. A decrease of ID 50, correlated with the age of the worms was observed: from 1·5 to 0·5 μg/ml for males and from 0·7 to 0·1 μg/ml for females. These results were confirmed in vivo by a higher efficacy of the anthelminthic at 21 than at 4 WPI.

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“…The mean larval position was calculated as (number of larvae per segment x distance of segment from stomach) divided by (total larvae x intestine length). Fourth-stage larvae were counted [12]. The small intestine of each infected mouse was removed, ligated at both ends with cotton twine to prevent contamination of the medium with digested matter and incubated for 2h at 37°C in Petri dishes containing 100μL RPMI 1640 Medium (Gibco, Paisley, UK) with 10% Glutamax (Gibco, Paisley, UK).…”

Section: Methodsmentioning

confidence: 99%

Colitis Promotes Adaptation of an Intestinal Nematode: A Heligmosomoides Polygyrus Mouse Model System

et al. 2013

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The precise mechanism of the very effective therapeutic effect of gastrointestinal nematodes on some autoimmune diseases is not clearly understood and is currently being intensively investigated. Treatment with living helminths has been initiated to reverse intestinal immune-mediated diseases in humans. However, little attention has been paid to the phenotype of nematodes in the IBD-affected gut and the consequences of nematode adaptation. In the present study, exposure of Heligmosomoides polygyrus larvae to the changed cytokine milieu of the intestine during colitis reduced inflammation in an experimental model of dextran sulphate sodium (DSS)- induced colitis, but increased nematode establishment in the moderate-responder BALB/c mouse strain. We used mass spectrometry in combination with two-dimensional Western blotting to determine changes in protein expression and changes in nematode antigens recognized by IgG1 in mice with colitis. We show that nematode larvae immunogenicity is changed by colitis as soon as 6 days post-infection; IgG1 did not recognize highly conserved proteins Lev-11 (isoform 1 of tropomyosin α1 chain), actin-4 isoform or FTT-2 isoform a (14-3-3 family) protein. These results indicate that changes in the small intestine provoked by colitis directly influence the nematode proteome. The unrecognized proteins seem to be key antigenic epitopes able to induce protective immune responses. The proteome changes were associated with weak immune recognition and increased larval adaptation and worm growth, altered localization in the intestine and increased survival of males but reduced worm fecundity. In this report, the mechanisms influencing nematode survival and the consequences of changed immunogenicity that reflect the immune response at the site colonized by the parasite in mice with colitis are described. The results are relevant to the use of live parasites to ameliorate IBD.

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Nematospiroides dubius: response of the late fourth-stage larva to anthelmintics in vitro

Cited by 11 publications

References 6 publications

Factors affecting the efficacy of ivermectin against Heligmosomoides polygyrus (Nematospiroides dubius) in mice

Factors affecting the efficacy of ivermectin against Heligmosomoides polygyrus (Nematospiroides dubius) in mice

The effect of levamisole on acetylcholinesterase secretion by male and female Heligmosomoides polygyrus during ageing

Colitis Promotes Adaptation of an Intestinal Nematode: A Heligmosomoides Polygyrus Mouse Model System

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