2000
DOI: 10.1074/jbc.275.14.10134
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neo-Inositol Polyphosphates in the AmoebaEntamoeba histolytica

Abstract: We have reexamined the structure of inositol phosphates present in trophozoites of the parasitic amoeba Entamoeba histolytica and show here that, rather than being myo-inositol derivatives (Martin, J.-B., BakkerGrunwald, T., and Klein, G. (1993) Eur. J. Biochem. 214, 711-718), these compounds belong to a new class of inositol phosphates in which the cyclitol isomer is neoinositol. The structures of neo-inositol hexakisphosphate, 2-diphospho-neo-inositol pentakisphosphate, and 2,5-bisdiphospho-neo-inositol tetr… Show more

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Cited by 37 publications
(39 citation statements)
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“…Soluble extract from 293 cells induced for 3 days with tetracycline was fractionated on a heparin-agarose column followed by a Mono Q column. Fractions from the Mono Q column (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were assayed for phosphorylation of Ins(1,3,4)P 3 and GST-AFT-2 (Fig. 5A).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Soluble extract from 293 cells induced for 3 days with tetracycline was fractionated on a heparin-agarose column followed by a Mono Q column. Fractions from the Mono Q column (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were assayed for phosphorylation of Ins(1,3,4)P 3 and GST-AFT-2 (Fig. 5A).…”
Section: Resultsmentioning
confidence: 99%
“…The two tetrakisphosphate products are produced in equal amounts from Ins(1,3,4)P 3 , but when Ins(1,4,5)P 3 is the substrate only Ins(1,3,4,5)P 4 is a product. The activity of the amoebae enzyme is very low compared with that of the human and plant enzymes, which may be because of the fact that the inositol polyphosphates found in E. histolytica are not myo-derivatives but are neo-derivatives (14). In addition to producing two distinct isomers of InsP 4 from a single substrate, it has been shown recently that 5/6-kinase can utilize inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P 4 ) as a substrate for 1-kinase activity to produce InsP 5 (15).…”
mentioning
confidence: 96%
“…d-chiro-IP 6 was reported in pine needles (L'Annunziata & Fuller 1971), but this was subsequently questioned on analytical grounds (Cosgrove 1980). Recently, neo-inositol phosphates have been identified in trophozoites of the parasitic amoebae Entamoeba histolytica and Phreatamoeba balamuthi using two-dimensional nuclear magnetic resonance (NMR) (Martin et al 2000). However, the fact that (i) terrestrial plants only contain the myo isomer of IP 6 and (ii) chemical epimerization of myo-IP 6 has been ruled out, suggest that microorganisms must play a key role in the synthesis of IP 6 isomers other than myo-IP 6 in the soil (Cosgrove 1969b).…”
Section: (Ii) Amounts and Forms Of Inositol Phosphates In Soilsmentioning
confidence: 99%
“…One has to point to these emerging myo-inositol pyrophosphates and their complexity as an area of great current chemical and biological activity and potential, but also note that neo-inositol IP 7 and IP 8 pyrophosphates are already known from protozoa, but are still of unknown function. [192] There are thus tantalising hints of wider activity already in evidence.…”
Section: Discussionmentioning
confidence: 99%
“…[192] The synthesis of neo-Ins(1,2,3,4,5,6)P 6 (137) and subsequent degradation to other neo-inositol phosphates by phytases has been described. [172] 9 muco-Inositol…”
Section: Neo-inositol Phosphatesmentioning
confidence: 99%