Neochlorogenic Acid Inhibits Lipopolysaccharide-Induced Activation and Pro-inflammatory Responses in BV2 Microglial Cells

Kim, Mina; Choi, Shin-Yang; Lee, Pyeongjae; Hur, Jinyoung

doi:10.1007/s11064-015-1659-1

Cited by 62 publications

(32 citation statements)

References 32 publications

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“…It can also inhibit α-glycosidase [48] and the activity of the influenza virus [49]. Moreover, the compound exerts neuroprotective effects by inhibiting the pathway of proinflammation in activated microglia cells [50]. The present results demonstrated the same properties for both Chlorogenic acid and Neochlorogenic acid.…”

Section: Dactylifric Acidsupporting

confidence: 71%

ADMET and pharmaceutical activity analysis of caffeic acid diversities by in silico tools

2020

Lett Appl NanoBioSci

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Caffeic acid diversities are widely considered as one of the most pharmaceutical secondary metabolites to study for treating a wide range of disorders and diseases. In this paper, toxicity, ADME, and pharmaceutical activity of 16 compounds of the Caffeic acid diversities, are analyzed by Toxtree software and Molinspiration website, respectively. According to the results, it can be concluded that Caffeoylmalic acid and Dactylifric acid could be considered as the safest and the most applicable compounds. It might be suggested that modification of molecular structures of Chlorogenic acid and Neochlorogenic acid could be useful for becoming low toxic and more applicable compounds for oral consumption.

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Section: Dactylifric Acidsupporting

confidence: 71%

ADMET and pharmaceutical activity analysis of caffeic acid diversities by in silico tools

2020

Lett Appl NanoBioSci

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“…In a previous study, chlorogenic acid suppressed glucose-induced glial cell activation (61). Moreover, neochlorogenic acid, an isomer of chlorogenic acid, inhibited the activation of lPS-induced microglia and p38 MaPK (62). catechin also inhibited lPS-induced no production in BV2 cells (63).…”

Section: Discussionmentioning

confidence: 88%

Anti‑inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide‑stimulated glial cells

et al. 2020

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Glial cells are the resident immune cells of the central nervous system. Reactive glial cells release inflammatory mediators that induce neurotoxicity or aggravate neurodegeneration. regulation of glial activation is crucial for the initiation and progression of neuropathological conditions. constituents of the peach tree (Prunus persica l. Batsch), which has a global distribution, have been found to exert therapeutic effects in pathological conditions, such as rashes, eczema and allergies. However, the therapeutic potential of its aerial parts (leaves, fruits and twigs) remains to be elucidated. The present study aimed to evaluate the anti-inflammatory role of P. persica methanol extract (PPB) on lipopolysaccharide (lPS)-stimulated glial cells. High-performance liquid chromatography coupled with tandem mass spectrometry analysis showed that PPB contained chlorogenic acid and catechin, which have antioxidant properties. Western blot and reverse transcription polymerase chain reaction results indicated that PPB reduced the transcription of various proinflammatory enzymes (nitric oxide synthase and cyclooxygenase-2) and cytokines [tumor necrosis factor-α, interleukin (il)-1β and il-6] in lPS-stimulated BV2 cells. in addition, PPB inhibited the activation of nF-κB and various mitogen-activated protein kinases required for proinflammatory mediator transcription. Finally, nitrite measurement and immunocytochemistry results indicated that PPB also suppressed nitrite production and nF-κB translocation in lPS-stimulated primary astrocytes. Thus, PPB may be used as a potential therapeutic agent for neurodegenerative diseases and neurotoxicity via the suppression of glial cell activation.

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“…If the repair process fails, it can induce apoptosis in many ways [47]. When cells are exposed to hypoxia and DNA damage, p53 can initiate exogenous apoptotic pathways by inducing Fas and FasL expression, interact with members of the Bcl-2 family, promote Bax expression, ultimately change the permeability of mitochondrial membrane and activate caspase in cell membrane, and induce cells to enter the mitochondria-mediated apoptotic pathway (i.e., the endogenous apoptotic pathway) [48]. e relation between p21 gene and tumor is mainly synergistic with the other related genes.…”

Section: Effects Of Avte On the Gene Expression Of The Apoptosis-relamentioning

confidence: 99%

In Vitro Analysis of Antioxidant, Anticancer, and Bioactive Components of Apocynum venetum Tea Extracts

Huang

Tan

et al. 2019

Journal of Food Quality

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The dry leaf of Apocynum venetum tea extracts (AVTEs) belonging to the Apocynaceae family is a traditional Chinese medicine. The aim of this study is to identify the bioactive components of AVTE and analyse its antioxidant and anticancer activity in vitro. Method. Flavones and polyphenols in AVTE were determined by high-performance liquid chromatography (HPLC) assay. The scavenging capacity of tea extracts to 1,1-diphenyl-2-picrylhydrazyl (DPPH); 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS); hydroxyl (OH); and superoxide anion-free radicals were investigated by spectrophotometry. We also detailed the cytotoxicity assay of AVTE (50, 100, and 200 μg/mL) to human embryonic kidney 293T cells, the protective effect of AVTE on 293T cells induced by hydrogen peroxide (0.3 mmol/L), and the anticancer effect against the human hepatoma HepG2 cells via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. We investigated the antioxidative effects of AVTE in human embryonic kidney 293T cells and the anticancer mechanism in HepG2 human hepatoma cells via quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) assay. Results. HPLC analysis showed that AVTEs contain neochlorogenic acid, chlorogenic acid, rutin, isoquercetin, isochlorogenic acid B, astragalin, isochlorogenic acid C, rosmarinic acid, quercetin, and trans-cinnamic acid. These extracts have high antioxidant activity and dose-dependent relation through free radical scavenging experiments. The cell viability of 293T cells treated with hydrogen peroxide (0.3 mmol/L) was significantly lower than that of normal cells, and the cell viability of oxidatively stressed 293T cells after AVTE (50, 100, and 200 μg/mL) treatment was significantly improved (P<0.05). Moreover, cytotoxicity experiments showed that the survival rate of 293T cells was over 90%, but the proliferation of HepG2 cells was significantly inhibited in a dose-dependent manner by AVTE. Furthermore, cytoprotective effects in 293T cells were induced via upregulation of glutathione peroxidase (GSH-Px), GSH, superoxide dismutase (SOD), and catalase (CAT) antioxidant-related factors, as well as apoptosis in HepG2 cells was induced via upregulation of caspase-3, caspase-9, p21, and p53 apoptosis-associated factors, as assessed via mRNA expression levels after treatment with AVTE, which were consistent with the results of antioxidant gene detections. As a conclusion, AVTE appears to be an effectively functional drink, due to its rich functional components and antioxidant and anticancer activities.

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Neochlorogenic Acid Inhibits Lipopolysaccharide-Induced Activation and Pro-inflammatory Responses in BV2 Microglial Cells

Cited by 62 publications

References 32 publications

ADMET and pharmaceutical activity analysis of caffeic acid diversities by in silico tools

ADMET and pharmaceutical activity analysis of caffeic acid diversities by in silico tools

Anti‑inflammatory role of Prunus persica L. Batsch methanol extract on lipopolysaccharide‑stimulated glial cells

In Vitro Analysis of Antioxidant, Anticancer, and Bioactive Components of Apocynum venetum Tea Extracts

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