Neocoleroa metrosideri sp. nov. (Sympoventuriaceae, Venturiales)

Abstract

Neocoleroa metrosideri is described as a new species and phylogenetically it is shown to belong in the Sympoventuriaceae, a recently established family, sister to the Venturiaceae. No other sequences are available for Neocoleroa but this new species is morphologically typical of the type species, with distinctive lobed to dichotomously branched, blunt-tipped setae on the superficial pseudothecia. The genus has previously been placed in the Pseudoperisporiaceae. This leaf spotting fungus is known from only a single specimen in an urban park but the same fungus has been detected also from two natural forest sites as an OTU in 454-based high throughput amplicon sequencing from DNA extracted from living leaves of Metrosideros excelsa.Keywords: LSU phylogeny, Metrosideros excelsa, New Zealand, Wentiomyces, 454 high throughput sequencingThe genera Neocoleroa Petrak (1934: 38) and Wentiomyces Koorders (1907: 168) have had a tangled taxonomic history. Kirk et al. (2008) Barr (1987, as Dimeriaceae) noted that some of these fungi are morphologically close to the Venturiaceae. This paper describes a new species Neocoleroa metrosideri, associated with leaf spots of Metrosideros excelsa. Morphologically it fits Barr's concept of Neocoleroa. DNA sequences from the type specimen and from cultures grown from single ascospores from the type, place this species in the Sympoventuriaceae, sister to the Venturiaceae in the Venturiales (Zhang et al. 2011). Materials and MethodsThe type specimen was examined when fresh, hymenial elements mounted in water; intact pseudothecia and adjacent host leaf tissues were rehydrated in 3% KOH and vertical sections were cut at a thickness of about 10 μm using a freezing microtome and mounted in lactic acid. Mature pseudothecia were crushed gently in 1% streptomycin solution, released ascospores streaked across a water agar plate and after 24 h single germinating ascospores transferred to Difco PDA, 2% Difco MEA, and Difco oatmeal agar plates. Cultures were described after 4 weeks. Specimens have been deposited in the PDD fungarium and ICMP culture collection.For DNA extraction, three separate extractions were done from single ascomata from three different leaves from PDD 107531 and from a culture derived from germinated ascospores from an ascoma from the collection. DNA was extracted and amplified using a REDExtract-N-Amp Plant PCR Kit (Sigma-Aldrich, USA), following the manufacturer's protocol except that the ascomata were ground in 30 μL extraction solution with a plastic pestle. Amplification primers for ITS were ITS1F and ITS4 (White et al. 1990, Gardes & Bruns 1993, for LSU were LR0R and LR5 (Vilgalys & Hester 1990, Bunyard et al. 1994. The DNA sequences from the all three fruiting bodies and from the culture were identical and have been accessioned in Genbank as KU131677 and KU131678.Additional LSU sequences were downloaded from Genbank, the taxon selection based on Machouart et al. (2014). LSU NEOCOLEROA METROSIDERI SP. NOV. Phytotaxa 253 (3) © 2016 Magnolia Press • 215sequences were a...

Venturiales

“…Barr (1987) noted that some species of these two genera are morphologically similar to members of Venturiaceae . Neocoleroa metrosideri was reported from Metrosideros excelsa , and morphologically agrees with Sympoventuria in having broadly clavate to obclavate asci, hyaline, 1-septate ascospores and persistent pseudoparaphyses ( Johnston & Park 2016 ). Phylogenetically, Neocoleroa metrosideri nested with a novel species N. cameroonensis described below, in Sympoventuriaceae , being sister to other genera of the family ( Fig.…”

“…Based on a multigene phylogenetic analysis, morphological and ecological comparisons, eight genera have been included in Sympoventuriaceae , viz ., Clavatispora , Ochroconis , Scolecobasidium , Sympodiella , Sympoventuria , Veronaeopsis , Verruconis and Yunnanomyces . Phylogenetically, Sympoventuriaceae forms a well-supported familial clade within Venturiales ( Arzanlou et al., 2007 , Crous et al., 2007a , Crous et al., 2007b , Zhang et al., 2011 , Samerpitak et al., 2014 , Johnston and Park, 2016 ).…”

“…2014 ). Neocoleroa metrosideri was described as a pathogen causing leaf spots on Metrosideros excelsa , which was widespread in M. excelsa forests in northern New Zealand ( Johnston & Park 2016 ). Although DNA sequences were not available for the type species of Neocoleroa , N .…”

“…sibirica , the comparable morphological characteristics with N . metrosideri argued for their congeneric status ( Barr, 1987 , Johnston and Park, 2016 ). Clavatispora was introduced as monotypic genus within Sympoventuriaceae represented by Clavatispora thailandica , which is characterised by its muriformly septate ascospores ( Boonmee et al.…”

Caracterización Molecular de la Diversidad Fúngica de los Bosques Llucud y Palictahua: Potencialidades en Control Biológico/Molecular Characterization of Diversity Fungic of the Llucud and Palictahua Forests: Potential in Biological Control

“…Fonsecaea pedrosoi: Uno de los principales agentes causales de cromoblastomicosis (micosis que afecta la piel y el tejido subcutáneo) en países tropicales(56). Neocoleroa metrosideri: Patógeno causante de manchas foliares(25).…”