Neoehrlichiosis in Symptomatic Immunocompetent Child, South Africa

Bamford, Colleen; Blumberg, Lucille; Bosman, Michelle; Frean, John; Hoek, Kim G.P.; Miles, Janet M.; Sriruttan, Charlotte; Vorster, Ilse; Oosthuizen, Marinda C.

doi:10.3201/eid2902.221451

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…A 1,467-bp 16S sequence amplified from a bone marrow aspirate from a patient from South Africa (GenBank accession no. OP208838) matched 100% over the 296-bp variable regions 1 and 2 target sequence amplified in this study ( 18 ). Scale bar indicates nucleotide substitutions per site.…”

Section: Resultsmentioning

confidence: 57%

“…CP014563.1; one sample had reads matching an uncultured and unnamed Neoehrlichia sp. previously identified in a patient from South Africa ( 18 ). Of the 10 participants with a detected bacterial zoonotic pathogen, 5 (50.0%) were male and 5 (50.0%) female; median age was 41 (IQR 32–56) years.…”

Section: Resultsmentioning

confidence: 84%

“…The specimen with MiniKraken taxonomic predictions of Anaplasmataceae, Ehrlichia ruminantium , and Ehrlichia ( Table 2 ) showed 100% sequence identity to a recently published novel Candidatus Neoehrlichia sp. sequence derived from a case of febrile illness in an immunocompetent child from South Africa who lived on a farm but had no reported tick bite ( 18 ). The 16S sequence from the patient from South Africa showed 100% identity to a 345-bp partial V3-V4 16S sequence (GenBank accession no.…”

Section: Discussionmentioning

confidence: 99%

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Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Rolfe,

Sheldon,

Kingry

et al. 2024

Emerg. Infect. Dis.

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Bacterial zoonoses are established causes of severe febrile illness in East Africa. Within a fever etiology study, we applied a high-throughput 16S rRNA metagenomic assay validated for detecting bacterial zoonotic pathogens. We enrolled febrile patients admitted to 2 referral hospitals in Moshi, Tanzania, during September 2007–April 2009. Among 788 participants, median age was 20 (interquartile range 2–38) years. We performed PCR amplification of V1–V2 variable region 16S rRNA on cell pellet DNA, then metagenomic deep-sequencing and pathogenic taxonomic identification. We detected bacterial zoonotic pathogens in 10 (1.3%) samples: 3 with Rickettsia typhi , 1 R. conorii , 2 Bartonella quintana , 2 pathogenic Leptospira spp., and 1 Coxiella burnetii . One other sample had reads matching a Neoerhlichia spp. previously identified in a patient from South Africa. Our findings indicate that targeted 16S metagenomics can identify bacterial zoonotic pathogens causing severe febrile illness in humans, including potential novel agents.

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Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Rolfe,

Sheldon,

Kingry

et al. 2024

Emerg. Infect. Dis.

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“…Over the past decade, symptomatic and asymptomatic infections with the tick-borne pathogen Candidatus Neoehrlichia mikurensis (CNM) have been increasingly reported [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. CNM was first discovered in 2004 in ticks and wild rodents on the Japanese island of Mikura [21] and first identified as a human pathogen in 2010 in a Swedish patient [1].…”

Section: Introductionmentioning

confidence: 99%

Fever of Unknown Origin, a Vascular Event, and Immunosuppression in Tick-Endemic Areas: Think About Neoehrlichiosis

Margini¹,

Maldonado²,

Keller³

et al. 2023

Cureus

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Three patients were referred to our hospital because of fever of unknown origin (FUO) and thrombosis or thrombophlebitis. All of them had been under immunosuppression (IS) with rituximab. Intensive diagnostics for FUO and blood cultures remained negative. Finally, the association of fever, immunosuppression, and a vascular event led to the suspicion of Candidatus Neoehrlichia mikurensis (CNM) infection. The diagnosis was confirmed by species-specific polymerase chain reaction (PCR) in the peripheral blood. Therapy with doxycycline or rifampicin led to the resolution of the disease. A liver biopsy was performed in one patient due to hepatomegaly and elevated liver enzymes demonstrating hemophagocytosis. To our knowledge, this is the first histopathological study of liver tissue in CNM infection. The evidence of hemophagocytosis raises the question of whether symptomatic CNM infection might be in part related to host inflammatory and immune responses.

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Clinical picture and outcome of the first identified case of human Neoehrlichia mikurensis infection in Finland

Hohenthal,

Tikkala,

Rinne

et al. 2025

Ticks and Tick-borne Diseases

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Neoehrlichiosis in Symptomatic Immunocompetent Child, South Africa

Cited by 3 publications

References 15 publications

Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007–20091

Fever of Unknown Origin, a Vascular Event, and Immunosuppression in Tick-Endemic Areas: Think About Neoehrlichiosis

Clinical picture and outcome of the first identified case of human Neoehrlichia mikurensis infection in Finland

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