Neogenin expression may be inversely correlated to the tumorigenicity of human breast cancer

Lee, Jeong Eon; Kim, Hee Joung; Bae, Ji‐Young; Kim, Seok Won; Park, Joon Suk; Shin, Hyuk Jai; Han, Wonshik; Kim, Sung Won; Kang, Kyung Sun; Noh, Dong‐Young

doi:10.1186/1471-2407-5-154

Cited by 30 publications

(34 citation statements)

References 15 publications

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“…Although there is currently no clear role for RNase 4 in cancer, there are instances where unusual RNase levels have been detected in cancer patients (Peracaula et al, 2000) and RNase 4 was downregulated in our metastasising sarcoma cells. Neogenin, which is downregulated in our metastatic cells, might play a role in mammary carcinogenesis, because its expression is inversely correlated with mammary carcinogenicity (Lee et al, 2005), which again supports the pattern of expression we report here.…”

Section: Journal Of Cell Science 120 (4)supporting

confidence: 89%

Evidence for protein 4.1B acting as a metastasis suppressor

Cavanna

Pokorná

Veselý

et al. 2007

Journal of Cell Science

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Section: Journal Of Cell Science 120 (4)supporting

confidence: 89%

Evidence for protein 4.1B acting as a metastasis suppressor

Cavanna

Pokorná

Veselý

et al. 2007

Journal of Cell Science

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Section: Agms In Breast Cancermentioning

confidence: 99%

“…Neo1 expression is lost in almost 95% of invasive ductal carcinomas (Table 2) [26]. Although loss of expression is not commonly seen in breast cancer cell lines, one study found that expression was lost in a breast cancer cell model where progressive environmental insults result in incremental increases in tumorigenicity and corresponding progressive decreases in Neo1 expression [26]. Knockout mouse studies have shown that NTN1/NEO1 interaction is necessary for the appropriate adhesion and stabilization of the highly proliferative cap cells of the TEB, and that loss of Ntn1 and Neo1 leads to breaks in the basal lamina, a phenomenon necessary for tumor progression [11].…”

Section: Agms In Breast Cancermentioning

confidence: 99%

Navigating Breast Cancer: Axon Guidance Molecules as Breast Cancer Tumor Suppressors and Oncogenes

Harburg

Hinck

2011

J Mammary Gland Biol Neoplasia

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Slit, Netrin, Ephrin, and Semaphorin’s roles in development have expanded greatly in the past decade from their original characterization as axon guidance molecules (AGMs) to include roles as regulators of tissue morphogenesis and development in diverse organs. In the mammary gland, AGMs are important for maintaining normal cell proliferation and adhesion during development. The frequent dysregulation of AGM expression during tumorigenesis and tumor progression suggests that AGMs also play a crucial role as tumor suppressors and oncogenes in breast cancer. Moreover, these findings suggest that AGMs may be excellent targets for new breast cancer prognostic tests and more effective therapeutic strategies.

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“…The expression levels of Neo1 vary significantly among different cancers. For example, high expression of Neo1 was found in ESCC [13], moderate expression was displayed in pancreatic cancer [11], and low expression was revealed in breast cancer, colon cancer and glioma [7,10,12]. Our study focused on the expression of Neo1 in GC.…”

Section: Discussionmentioning

confidence: 99%

Neogenin-1 Promotes Cell Proliferation, Motility, and Adhesion by Up-Regulation of Zinc Finger E-Box Binding Homeobox 1 Via Activating the Rac1/PI3K/AKT Pathway in Gastric Cancer Cells

Qu¹,

Sun²,

Wang³

2018

Cell Physiol Biochem

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Background/Aims: Neogenin-1 (Neo1) has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration. The present study aimed to explore the functional role of neogenin-1 (Neo1) in gastric cancer (GC), together with underlying mechanisms. Methods: Neo1 expression was analyzed by qRT-PCR and Western blot analysis in both human GC cell lines and normal gastric epithelial cell line. Neo1 was respectively overexpressed or silenced by transfection with pcDNA3.1 or siRNA, and then the cells were incubated with or without different concentrations of cisplatin, transforming growth factor (TGF)-β1, and/or inhibitors of Rac-1 and PI3K. Thereafter, cell viability, invasion, and adhesion were measured by CCK-8, wound healing and adhesion assays, respectively. The expression levels of key factors involved in epithelial mesenchymal transition (EMT) and the PI3K/AKT pathway were analyzed by Western blot analysis. Results: The results showed that the Neo1 level was significantly increased in GC cell lines, with the highest level in SGC-7901 cells. Overexpression of Neo1 significantly reduced the GC cell sensitivity to cisplatin and increased the cell viability, motility and adhesion ability, and while silencing of Neo1 showed contrary results. Moreover, overexpression of Neo1 dramatically downregulated the E-Cadherin level and upregulated the levels of N-Cadherin and Vimentin. In addition, the data revealed that Neo1 positively regulated the expression of Zinc finger E-box-binding homeobox 1 (ZEB1) by activating the Rac1/PI3K/AKT pathway. Conclusions: Neo1 could promote cell proliferation, motility, and adhesion by up-regulation of ZEB1 via activating the Rac1/PI3K/AKT pathway in GC cells.

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Neogenin expression may be inversely correlated to the tumorigenicity of human breast cancer

Abstract: Background: Neogenin is expressed in cap cells that have been suggested to be mammary stem or precursor cells. Neogenin is known to play an important role in mammary morphogenesis; however its relationship to tumorigenesis remains to be elucidated.

Cited by 30 publications

References 15 publications

Evidence for protein 4.1B acting as a metastasis suppressor

Evidence for protein 4.1B acting as a metastasis suppressor

Navigating Breast Cancer: Axon Guidance Molecules as Breast Cancer Tumor Suppressors and Oncogenes

Neogenin-1 Promotes Cell Proliferation, Motility, and Adhesion by Up-Regulation of Zinc Finger E-Box Binding Homeobox 1 Via Activating the Rac1/PI3K/AKT Pathway in Gastric Cancer Cells

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