Neoglycosylated Collagen: Effect on Neuroblastoma F-11 Cell Lines

Abstract

The regeneration of the nervous system is a challenging task. Currently, regenerative medicine approaches that exploit nature-inspired cues are being studied and hold great promise. The possibility to use protein-based matrices functionalized with small oligo- and monosaccharides is of interest since these can be finely tuned to better mimic the native environment. Collagen has been selected as a promising material that has the potential to be further tailored to incorporate carbohydrates in order to drive cell behavior towards neuroregeneration. Indeed, the grafting of carbohydrates to collagen 2D matrices is proved to enhance its biological significance. In the present study, collagen 2D matrices were grafted with different carbohydrate epitopes, and their potential to drive F-11 neuroblastoma cells towards neuronal differentiation was evaluated. Collagen functionalized with α-glucosides was able to differentiate neuroblastoma cells into functional neurons, while sialyl α-(2→6)-galactosides stimulated cell proliferation.

Chitosan-Based Biomaterials: Insights into Chemistry, Properties, Devices, and Their Biomedical Applications

“…Innovative wound dressing materials are designed to address these issues; in this framework, hydrogels offer the advantage of their degradation ability. Additionally, hydrogel properties for damaged tissue regeneration may be improved owing to their ability to act as delivery vehicles of biomolecules for promoting tissue repair (i.e., growth factors, immunomodulators, glycans) [ 148 , 149 , 150 ], and limit bacterial infections (i.e., acting as anti-bacterial agents). The wound dressings based on CHT hydrogels are intrinsically biodegradable and antimicrobial [ 151 ], rendering them suited for wound healing and tissue regeneration.…”