Neomangiferin modulates the Th17/Treg balance and ameliorates colitis in mice

Lim, Seungyup; Kang, Geum-Dan; Jeong, Jin-Ju; Choi, Hyun Sik; Kim, Dong-Hyun

doi:10.1016/j.phymed.2016.01.002

Cited by 43 publications

(33 citation statements)

References 42 publications

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“…Mouse spleens were aseptically removed, gently crushed, and lysed in tris-buffered ammonium chloride (Biosesang, Seoul, Korea) according to the method of Lim et al [20]. Cells were suspended in RPMI 1640 medium containing 10% FCS and T cells were isolated by magnetic cell sorting with the Pan T Cell Isolation Kit II.…”

Section: Methodsmentioning

confidence: 99%

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Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 Attenuate Gardnerella vaginalis-Infected Bacterial Vaginosis in Mice

Jang

Jeong

Choi³

et al. 2017

Nutrients

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Oral administration of a probiotic mixture (PM; Respecta®) consisting of Lactobacillus rhamnosus HN001 (L1), Lactobacillus acidophilus La-14 (L2), and lactoferrin RCXTM results in colonization of these probiotics in the vagina of healthy women. Therefore, we examined whether vaginal colonization of the PM ingredients L1 and L2 could attenuate bacterial vaginosis (BV). BV was induced in mice via β-estradiol-3-benzoate-induced immunosuppression and intravaginal inoculation with Gardnerella vaginalis (GV). Inflammatory markers were analyzed using enzyme-linked immunosorbent assay, immunoblotting, quantitative polymerase chain reaction, and flow cytometry. Oral or intravaginal administration of PM resulted in colonization of L1 and L2 in the vagina. Oral or intravaginal administration of L1, L2, or PM significantly inhibited GV-induced epithelial cell disruption, myeloperoxidase activity, NF-κB activation, and IL-1β and TNF-α expression (p < 0.05). Administration of these probiotics also inhibited IL-17 and RORγt expression but increased IL-10 and Foxp3 expression. Of these probiotics, L2 most effectively attenuated GV-induced BV, followed by L1 and PM. Oral administration was more effective against GV-induced BV than intravaginal administration. L1 and L2 also significantly inhibited the adherence of GV to HeLa cells (a human cervical cancer cell line) and GV growth in vitro. In addition, L1 and L2 inhibited lipopolysaccharide-induced NF-κB activation in macrophages and the differentiation of splenocytes into Th17 cells in vitro, but increased their differentiation into Treg cells. Our study suggests that L1, L2, and PM attenuated GV-induced vaginosis by regulating both vaginal and systemic innate and adaptive immune responses rather than direct competition or killing of GV in the vagina.

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Section: Methodsmentioning

confidence: 99%

“…Real-time PCR analysis of IL-10, RAR-related orphan receptor gamma t (RORγt), Foxp3, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was performed according to the method of Lim et al [20]. Total RNA was isolated from the vagina using the RNeasy Mini Kit, and cDNA was prepared from 2 μg of purified RNA using Takara reagents.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 Attenuate Gardnerella vaginalis-Infected Bacterial Vaginosis in Mice

Jang

Jeong

Choi³

et al. 2017

Nutrients

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“…Previous studies identified immune dysfunction in patients with acute bronchitis, in which a dysfunction in the type 1 T-helper cell (Th1)/Th2 subpopulation was mostly studied in the pathogenesis of acute bronchitis. After T-regulatory (Treg) and Th17 cells were successively identified, the imbalance of Treg/Th17 and cytokines has become a theoretical basis for the study of immune disorders and other diseases (11,12). In the present study, the expression of Treg/Th17 and cytokines in peripheral blood were detected in children with acute bronchitis, in order to further investigate the role of Treg/Th17 in the pathogenesis of acute bronchitis and its clinical significance.…”

Section: Introductionmentioning

confidence: 93%

Changes of Treg and Th17 cells as well as cytokines in children with acute bronchitis

Xue

Chen

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to investigate changes of T-regulatory (Treg) and T-helper (Th)17 cells as well as cytokines in peripheral blood of children with acute bronchitis, and to explore the roles of these cells in the pathogenesis of acute bronchitis. A total of 126 children who had presented at Renji Hospital (Shanghai, China) with acute bronchitis were selected as the observation group and 30 healthy children were selected as the control group. Th17/Tregs in the peripheral blood of the children of the observation group and the control group was detected by flow cytometry. The levels of cytokines interleukin (IL)-17, IL-22, IL-10 and transforming growth factor (TGF)-β in peripheral blood serum were detected by ELISA. Compared with those in the control group, Treg cells, the Treg/Th17 ratio as well as serum IL-10 and TGF-β levels were significantly decreased in the observation group (P<0.05), while Th17 cells as well as serum levels of IL-17 and IL-22 were significantly increased (P<0.05). In conclusion, Treg/Th17 and the expression of associated cytokines lost their balance in children with acute bronchitis, suggesting that Treg and Th17 cells as well as their cytokines may be involved in the pathogenesis of acute bronchitis. It may be of certain guiding significance to detect Treg/Th17 and levels of serum cytokines in peripheral blood for clinical treatment.

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“…Pharmacological studies showed that phenolic glycosides from Baihe such as regaloside A (RA) and regaloside I (RI) (Kwon et al, ; Lee, Yun, Jang, & Kim, ), xanthones from Zhimu [e.g. neomangiferin (NG) and mangiferin (MG)] (Jangra, Lukhi, Sulakhiya, Baruah, & Lahkar, ; Lim, Kang, Jeong, Choi, & Kim, ; Marquez, Garcia‐Bueno, Madrigal, & Leza, ) as well as steroidal saponins from Zhimu like timosaponin B‐II (TB‐II), anemarsaponin E (AE), timosaponin AIII (TA III) and sarsasapogenin (SP) (Lei et al, ; Lim et al, ; Lu et al, ) have significant anti‐inflammatory properties. Consequently, the above compounds are speculated to be the representative ingredients that contribute to the pharmacodynamic material basis of BZD in depression therapy.…”

Section: Introductionmentioning

confidence: 99%

Chemical interaction between Lilium brownii and Rhizoma Anemarrhenae, the herbal constituents of Baihe Zhimu decoction, by liquid chromatography coupled to hybrid triple quadrupole linear ion trap mass spectrometer

Yang

Liu

Wang

et al. 2017

Biomedical Chromatography

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During the course of decoction, the components of herbal formula interact with each other, such that chemical extraction characteristics are altered. The crude drugs, Lilium brownii (Baihe) and Rhizoma Anemarrhenae (Zhimu), are the herbal constituents of Baihe Zhimu decoction, a traditional herbal formula. To investigate the chemical interaction between Baihe and Zhimu when decocting together, eight marker components in Baihe Zhimu decoction were simultaneously characterized and quantified in one run by a hybrid triple quadrupole linear ion trap mass spectrometer in the multiple reactions monitoring-information dependent acquisition-enhanced product ion mode. The results showed that Zhimu significantly suppressed the extraction of phenolic glycosides (the components from Baihe) when co-decocting, and Baihe clearly suppressed the extraction of xanthones and steroidal saponins (the components from Zhimu). Overall, the presently developed method would be a preferred candidate for the investigation of the chemical interaction between herbal medicines.

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Neomangiferin modulates the Th17/Treg balance and ameliorates colitis in mice

Cited by 43 publications

References 42 publications

Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 Attenuate Gardnerella vaginalis-Infected Bacterial Vaginosis in Mice

Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus La-14 Attenuate Gardnerella vaginalis-Infected Bacterial Vaginosis in Mice

Changes of Treg and Th17 cells as well as cytokines in children with acute bronchitis

Chemical interaction between Lilium brownii and Rhizoma Anemarrhenae, the herbal constituents of Baihe Zhimu decoction, by liquid chromatography coupled to hybrid triple quadrupole linear ion trap mass spectrometer

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