Neomycin—production and Antibiotic Properties 123

Waksman, Selman A.; Lechevalier, H. A.; Harris, Dale A.

doi:10.1172/jci102182

Cited by 49 publications

(22 citation statements)

References 4 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The negativecontrol group of mice (untreated group) was given PBS instead of antimicrobial agent, whereas the positive-control group was treated with neomycin sulfate antibiotic at 10 mg · kg Ϫ1 (antibiotic-treated group). Neomycin sulfate is an antibiotic from the aminogylcoside family that is used in topical ointments, and this has been used to study S. aureus infections (47,48). The bacterial recoveries for the antibiotic-treated group and the untreated group were found to be 4.1 Ϯ 1.0 and 10.9 Ϯ 0.3 log 10 CFU/g of tissue, respectively.…”

Section: Synthesis Of Antimicrobial Peptidesmentioning

confidence: 99%

In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization

Malik

Fensterseifer

Chan

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Staphylococcus aureus is a virulent pathogen that is responsible for a wide range of superficial and invasive infections. Its resistance to existing antimicrobial drugs is a global problem, and the development of novel antimicrobial agents is crucial. Antimicrobial peptides from natural resources offer potential as new treatments against staphylococcal infections. In the current study, we have examined the antimicrobial properties of peptides isolated from anuran skin secretions and cyclized synthetic analogues of these peptides. The structures of the peptides were elucidated by nuclear magnetic resonance (NMR) spectroscopy, revealing high structural and sequence similarity with each other and with sunflower trypsin inhibitor 1 (SFTI-1). SFTI-1 is an ultrastable cyclic peptide isolated from sunflower seeds that has subnanomolar trypsin inhibitory activity, and this scaffold offers pharmaceutically relevant characteristics. The five anuran peptides were nonhemolytic and noncytotoxic and had trypsin inhibitory activities similar to that of SFTI-1. They demonstrated weak in vitro inhibitory activities against S. aureus, but several had strong antibacterial activities against S. aureus in an in vivo murine wound infection model. pYR, an immunomodulatory peptide from Rana sevosa, was the most potent, with complete bacterial clearance at 3 mg · kg ؊1 . Cyclization of the peptides improved their stability but was associated with a concomitant decrease in antimicrobial activity. In summary, these anuran peptides are promising as novel therapeutic agents for treating infections from a clinically resistant pathogen. Staphylococcus aureus is one of the most virulent and opportunistic pathogens, causing increasing numbers of nosocomial and community-acquired infections, and is a leading cause of skin and soft tissue infections (1-3). These Gram-positive cocci produce a myriad of virulence factors that allow the bacteria to attach to host cells, to invade tissues, to evade the host immune system, and to release an array of exoproteins and toxins (4, 5). Contagious S. aureus skin infections can lead to severe muscle or bone infections that ultimately can spread to the lungs or heart (6). The primary treatment involves prescription of ␤-lactam antibiotics such as penicillins and cephalosporins, along with clinical woundcleaning procedures (6, 7). Strains resistant to antibiotics have been emerging since the 1960s, however, especially methicillinresistant S. aureus (MRSA), which is most common in nosocomial skin infections (8). Alarmingly, there have been reports of S. aureus strains that are resistant to the drug of last resort, vancomycin (9).Antimicrobial peptides are now recognized as novel alternative therapeutic agents for infection control (10). Several hypotheses have been examined regarding the mode of action of antimicrobial peptides, including autolysin activation, lipopolysaccharide (LPS) permeabilization, fatal depolarization of the energized bacterial membrane, formation of barrel-stave pores that cause leakage of...

show abstract

Section: Synthesis Of Antimicrobial Peptidesmentioning

confidence: 99%

In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization

Malik

Fensterseifer

Chan

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…isolated a Str e ptomycete (No. 3535) (closely related to S.fradiae) which produced a new antibiotic, neomycin (69,70,71). The crude material was active both in vitro and in vivo against a variety of gram positive and gram negative organisms, including Mycobacterium tuberculosis and other mycobacteria.…”

Section: Compounds Containing Carbon Hydrogen Oxygen Nitrogenmentioning

confidence: 99%

Biochemistry of Antibiotics

Carter¹,

Ford²

1950

Annu. Rev. Biochem.

View full text Add to dashboard Cite

“…The binding of neomycin, and other aminoglycosides drugs, to bacterial RNA affects the production of proteins through miscoding in translation, ultimately leading to bacterial death. 2–5 In addition to binding to ribosomal RNA, aminoglycosides have been shown to bind numerous other RNA, 6–10 DNA, 11–17 hybrid duplex, 18,19 triplex 20–23 and quadruplex 24–26 structures of therapeutic interest. 27 …”

Section: Introductionmentioning

confidence: 99%

Utilization of chromic polydiacetylene assemblies as a platform to probe specific binding between drug and RNA

et al. 2017

View full text Add to dashboard Cite

Recognition of nucleic acids remains an important endeavor in biology. Nucleic acids adopt shapes ranging from A-form (RNA and GC rich DNA) to B-form (AT rich DNA). We show, in this contribution, shape-specific recognition of A-U rich RNA duplex by a neomycin (Neo)-polydiacetylene (PDA) complex. PDA assemblies are fabricated by using a well-known diacetylene (DA) monomer, 10,12-pentacosadiynoic acid (PCDA). The response of poly(PCDA) assemblies is generated by mixing with a modified neomycin-PCDA monomer (Neo-PCDA). The functionalization by neomycin moiety provides specific binding with homopolyribonucleotide poly (rA) - poly (rU) stimulus. Various types of alcohols are utilized as additives to enhance the sensitivity of poly(PCDA)/Neo-PCDA assemblies. A change of absorption spectra is clearly observed when a relatively low concentration of poly (rA)-poly (rU) is added into the system. Furthermore, poly(PCDA)/Neo-PCDA shows a clear specificity for poly (rA)-poly (rU) over the corresponding DNA duplex. The variation of linker between neomycin moiety and conjugated PDA backbone is found to significantly affect its sensitivity. We also investigate other parameters including the concentration of Neo-PCDA and the DA monomer structure. Our results provide here preliminary data for an alternative approach to improve the sensitivity of PDA utilized in biosensing and diagnostic applications.

show abstract

Neomycin—production and Antibiotic Properties 123

Cited by 49 publications

References 4 publications

In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization

In Vivo Efficacy of Anuran Trypsin Inhibitory Peptides against Staphylococcal Skin Infection and the Impact of Peptide Cyclization

Biochemistry of Antibiotics

Utilization of chromic polydiacetylene assemblies as a platform to probe specific binding between drug and RNA

Contact Info

Product

Resources

About