Neonatal exposure to coumestrol, a phytoestrogen, does not alter spermatogenic potential in rats

Awoniyi, Caleb A.; Roberts, Dawn; Chandrashekar, Varadaraj; Veeramachaneni, D. N. Rao; Hurst, Bradley S.; Tucker, Kathleen; Schlaff, William D.

doi:10.1007/bf02801328

Cited by 27 publications

(13 citation statements)

References 41 publications

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“…In mammals (rats) the same effects of dietary phytoestrogens on the male reproductive tract have been noted (Atanassova et al, 2000;Delclos et al, 2001;Weber et al, 2001;Wisniewski et al, 2003). In contrast, no effects were observed for dietary genistein (Nagao et al, 2001;Fritz et al, 2003) or coumestrol (Awoniyi et al, 1997). In our study, we observed no differences in basal and LH-stimulated testosterone secretion by Leydig cells from ganders fed both control diets and diets with a higher phytoestrogen content.…”

Section: Discussioncontrasting

confidence: 60%

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Opałka¹,

Kamińska²,

Piskuła

et al. 2006

British Poultry Science

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The aim of this study was to examine ganders at different stages of the reproductive season and the effect of: (1) diets with high phytoestrogen content and (2) in vitro phytoestrogen treatment on testosterone secretion by isolated Leydig cells. Thirty-six male Biłgoraj geese were fed control diets with low phytoestrogen content (containing grass meal) and diets with high phytoestrogens (containing alfalfa meal and soy). Testes were obtained from both groups of ganders at three different times of the breeding season: peak of reproductive activity (March), second half of reproductive activity (May) and beginning of photorefractoriness (July). Isolated Leydig cells were incubated with LH as well as genistein, daidzein, equol and coumestrol and the concentration of testosterone in the medium was determined by radioimmunoassay. The mean weight of testes from ganders in May and July decreased relative to their weights in March, but no significant differences among experimental groups were noted. No differences were observed in basal and LH-stimulated testosterone secretion by Leydig cells of ganders fed the control diets and the diets with higher phytoestrogen content. In July, LH did not stimulate testosterone secretion in either group. In vitro treatment with genistein, daidzein and equol (5 and 50 microM) inhibited basal and LH-stimulated testosterone production by Leydig cells from both groups. Coumestrol (5 and 50 microM) inhibited basal testosterone secretion only in March in the control group. Dietary exposure to phytoestrogens had a slight effect on in vitro testicular secretion in ganders. In vitro treatment with phytoestrogen inhibited testosterone production by Leydig cells. Genistein showed the strongest effect and coumestrol had the weakest influence on testicular secretion.

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Section: Discussioncontrasting

confidence: 60%

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Opałka¹,

Kamińska²,

Piskuła

et al. 2006

British Poultry Science

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“…The results of the study showed no significant body weight gain, also like in other studieswhere no significant differences in the animal body weights were observed (Awoniyi et al, 1997). Ukwenya et al, that profertility effects of alcoholic extract of sesame in male sprague-dawley rats studied, they found that the effects of ethanolic extract of beniseed (sesame) at 3000 mg/kg body weight, with vitamin C administered as adjuvant, have shown that beniseed has a potential to increase mean body weights of rats.…”

Section: Serum Hormone (Lh Fsh and T) Levelssupporting

confidence: 85%

Effect of Diet Contains Sesame Seed on Epididymal Histology of Adult Rat

Mahabadi

Khodayari

et al. 2013

Int. J. Morphol.

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SUMMARY:Sesamin is a major lignan constituent of sesame seed and considered as a key factor in a number of beneficial effects on human health. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male sprague dawley rat. The present study was aimed to investigate the effects of contain sesame seed on epididymis histologhical structure. Thirty adult male rats were divided into two groups of 15 rats each. The regimen group received diet containing 30% sesame seed, while the control group received diet along 12 weeks. The right epididymis were removed and minced into several pieces on a specimen bottle containing normal saline for some few minutes to allow the sperms to become motile and swim out and sperm parameters were analyzed. Left epididymis were divided into three sections and fixed into bouin,s solution for further investigation. Serum FSH and LH concentration were estimated by ELISA technique, Testosterone concentration was done by using Chemo-Luminance method. The body weight gained during the treatment period did not differ significantly among groups. The mean epididymal sperm motility and count of the experimental group was significantly higher than control group. LH levels significantly increased in experimental group compared to controls but significant changes in FSH and testosterone levels were not observed in both groups. The results obtained showed that the mean epididymal diameter of the tubular, lumen and epithelium in three parts were not significant in two groups. It can be concluded that sesame seed improves sperm parameters (motility and count) and also can increase LH. But diet contains sesame seed did not affect on epididymal tissue and body weight.

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“…In this context, epididymal and testicular sperm counts were not altered in rats administered genistein orally at doses of 12.5-100 mg/kg on postnatal days 1-5 (Nagao et al, 2001), 10-1000 μg/day (Shibayama et al, 2001) or 100 μg/day of coumestrol (Awoniyi et al, 1997). This was also true in rats chronically administered genistein at a dose of 50 μg/day (Roberts et al, 2000), 0.1-10 mg/kg/day through pregnancy and lactation (Fielden et al, 2003), 200 and 2000 mg of genistein per kg of diet for 12 months (Faqi et al, 2004) or 2.5 mg/kg/day for 5 months (Lee et al, 2004).…”

Section: Discussionmentioning

confidence: 85%

Effects of chronic exposure to soy meal containing diet or soy derived isoflavones supplement on semen production and reproductive system of male rabbits

Cardoso¹,

Báo²

2007

Animal Reproduction Science

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Effects of chronic exposure to soy meal containing diet or soy derived isoflavones supplement on semen production and reproductive system of male rabbits Julio Roquete Cardoso Sonia Nair Báo AbstractSoy and derivative diets deliver large doses of isoflavones to human and animals throughout their lifespan, including gestation. Epidemiologic and experimental data suggest that the consumption of soybean containing foods may protect against cardiovascular disease and decrease breast, prostate and endometrial cancer risk. Based on animal and in vitro studies, however, concerns have been raised that consumption of isoflavones may cause potential adverse effects on the reproductive tract and behavior. The aim of this study was to investigate the effects of chronic consumption of a soy meal containing diet or soy isoflavones supplement on the morphology of reproductive organs, semen quality, age that males reached puberty, and sexual behavior of male rabbits. With this purpose, 16 female rabbits were randomly assigned to receive: (1) a soy-and alfafa-free diet; (2) a soy-and alfafa-free diet supplemented with 5 mg/kg body wt./day of soy isoflavones; (3) a soy-and alfafa-free diet supplemented with 20 mg/kg body wt./day of soy isoflavones; (4) a diet containing 18% of soy meal, throughout the gestation and lactation. After weaning, male offspring received the same diet, which was given to the respective mother. The age that males reached puberty, semen characteristics and sexual behavior were evaluated in these animals. At 33 weeks of age, the reproductive organs were submitted to histological evaluation. Rabbits, which received large amounts of isoflavones (20 mg/kg body wt./day) had a lesser food intake, body weight and semen volume. Spermatogenesis, morphology of male genital organs and sexual behavior did not differ significantly from the control group. We conclude that chronic dietary treatment with soy based diet or soy isoflavones have no adverse effects on the observed reproductive patterns of male rabbits.

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Neonatal exposure to coumestrol, a phytoestrogen, does not alter spermatogenic potential in rats

Cited by 27 publications

References 41 publications

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Effect of Diet Contains Sesame Seed on Epididymal Histology of Adult Rat

Effects of chronic exposure to soy meal containing diet or soy derived isoflavones supplement on semen production and reproductive system of male rabbits

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