Neonatal Genistein Chemoprevents Mammary Cancer

Lamartiniere, Coral A.; Moore, Judson B.; Holland, Michael; Barnes, Stephen

doi:10.3181/00379727-208-43843

Cited by 221 publications

(123 citation statements)

References 0 publications

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…According to experimental (Lamartiniere et al, 1995;Murrill et al, 1996;Hilakivi-Clarke et al, 1999) and epidemiological studies (Shu et al, 2001;Wu et al, 2002), the timing of soy exposure during developmental years may be crucial for the protective effects from soy. In animals, prepubertal exposure to soy appears to be more effective than adult exposure in reducing mammary tumor formation (Lamartiniere et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Urinary isoflavone excretion as a compliance measure in a soy intervention among young girls: a pilot study

et al. 2004

View full text Add to dashboard Cite

Objective: To investigate the compliance of young girls with a soy intervention. Design: An 8-week dietary intervention and urine sample collection. Setting: Free-living girls. Subjects: A convenience sample of 8-to 14-y-old girls (20 started and 17 finished the study) recruited through flyers distributed to staff members and previous study participants. Intervention: The girls consumed one daily serving of soymilk, soy nuts, or tofu, completed 3-day food records, kept daily soy intake logs, and collected weekly urine samples. Main outcome measures: Compliance with the intervention was evaluated by daily soy intake logs, 3-day food records analyzed by the center's Food Composition and Food Groups Servings Databases, and weekly urinary isoflavone excretion using high-pressure liquid chromatography. The statistical analysis included paired t-tests, analysis of variance, and Spearman's rankorder correlation coefficients. Results: Daily soy intake logs indicated a mean intake of 6.28 servings out of a maximum of 7.0 servings per week. The food records revealed a six-fold increase in isoflavone intake during the study period (Po0.01) which was confirmed by an increase in urinary isoflavone excretion of similar magnitude (23.3-142.1 nmol/mg creatinine, P ¼ 0.02). Conclusions: This study demonstrated the ability of young girls to consume one daily soy serving and the usefulness of urinary isoflavones as a primary compliance measure. The high urinary isoflavone excretion levels detected in girls as compared to adult women suggest less intestinal degradation and/or greater absorption of isoflavones in nonadult populations. This finding requires further investigations into the pharmacokinetics of isoflavones.

show abstract

Section: Introductionmentioning

confidence: 99%

Urinary isoflavone excretion as a compliance measure in a soy intervention among young girls: a pilot study

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Soy-based diets are high in iso¯avonoid. Genistein is a prominent iso¯avonoid in soy products and has been proposed as the agent responsible for lowering the rate of breast cancer in Asian women (Lamartiniere et al, 1995;Fotsis et al, 1995). The mechanism for this chemopreventive e ect, however, is unknown.…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that genistein is a potent inhibitor of cell proliferation, oncogenesis and clonogenic ability in animal and human cells (Fotsis et al, 1995;Barnes, 1995). Genistein also inhibits the growth of carcinogeninduced cancers in rats and human leukemia cells transplanted into mice (Lamartiniere et al, 1995;Uckun et al, 1995). The induction of stress proteins (e.g.…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis in breast cancer cells MDA-MB-231 by genistein

et al. 1999

View full text Add to dashboard Cite

Breast cancer is the most common cancer among American women, whereas Asian women, who consume a traditional diet high in soy products, have a relatively low incidence. Genistein is a prominent iso¯avonoid in soy products and has been proposed as the agent responsible for lowering the rate of breast cancer in Asian women. We investigated the e ects of genistein on cell growth and apoptosis-related gene expression in breast cancer cells MDA-MB-231. We found upregulation of Bax and p21 WAF1 expressions and downregulation of Bcl-2 and p53 expression in genisteintreated cells. Furthermore, DNA ladder formation, CPP32 activation, and PARP cleavage were observed after treatment with genistein, indicating apoptotic cell deaths. Flow cytometry with 7-amino actinomycin D staining showed that the number of apoptotic cells increased with longer treatment of genistein. From these results, we conclude that genistein inhibits the growth of MDA-MB-231 breast cancer cells, regulates the expression of apoptosis-related genes, and induces apoptosis through a p53-independent pathway. The up-regulation of Bax and p21 WAF1 may be the molecular mechanisms by which genistein induces apoptosis, however, further de®nitive studies are needed. These results suggest that genistein may be a potentially e ective chemopreventive or therapeutic agent against breast cancer.

show abstract

“…A myriad of experimental studies were performed to explore effects of phytoestrogens on the structures and functions of the reproductive system and related mechanisms. To date, there is evidence that phytoestrogens (such as genistein, coumestrol, quercetin), given in high doses or at critical stages of development in experi-mental animals can result in severe reproductive tract disorders (Lamartiniere et al, 1995;Strauss et al, 1998;Tou et al, 1999), and temporary infertility syndromes in domestic animals have been related to high phytoestrogens consumption in grazing (Adams, 1995).…”

Section: Introductionmentioning

confidence: 99%