Neonatal group B streptococcal disease in Finland: a ten-year nationwide study

Kalliola, Satu; Vuopio‐Varkila, Jaana; Takala, Aino K.; Eskola, Juhani

doi:10.1097/00006454-199909000-00012

Cited by 91 publications

(69 citation statements)

References 34 publications

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“…Sequencing of the 790-bp PCR amplicon allows MS III to be subtyped on the basis of the sequence heterogeneity. Previous studies in the United States and several other countries have shown that serotypes Ia, Ib, II, III, and V are those most frequently isolated from normally sterile sites (9,11). Serotypes VI and VIII are the predominant serotypes isolated from patients in Japan (15) but are uncommon elsewhere.…”

Section: Discussionmentioning

confidence: 99%

Serotype Identification of Group B Streptococci by PCR and Sequencing

et al. 2002

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Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes Ia to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes Ia to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera. A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Serotype Identification of Group B Streptococci by PCR and Sequencing

et al. 2002

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“…Varios estudios han señalado, en las últimas dos décadas, un aumento en la incidencia del serotipo III en infecciones neonatales y maternas, constituyendo el serotipo más frecuentemente aislado en casos de sepsis neonatal y de meningitis 8,13,14,23 .…”

Section: Discussionunclassified

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

et al. 2004

Rev. méd. Chile

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“…In Th-1 immune responses, IL-18 and IL-12 are important cytokines that may synergistically stimulate IFN-␥ production and enhance NK and T cell-mediated cytotoxicity (19). Recent studies implicate the interaction of these Th-1 cytokines in the development of autoimmune diseases and suggest that regulating their function may be therapeutically beneficial (20,21).Early-onset GBS infections in neonates often lead to sepsis and severe septic shock, with an approximate 5-15% mortality rate (22)(23)(24) …”

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“…Early-onset GBS infections in neonates often lead to sepsis and severe septic shock, with an approximate 5-15% mortality rate (22)(23)(24) …”

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Defective Production of IL-18 and IL-12 by Cord Blood Mononuclear Cells Influences the T Helper-1 Interferon Gamma Response to Group B Streptococci

et al. 2003

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Human neonates are uniquely susceptible to group B streptococcal (GBS) infections. We have shown that neonatal mixed mononuclear cells have a deficiency in the production of the T helper-1 (Th-1) cytokine, interferon gamma (IFN-␥), and that incubation of neonatal neutrophils with recombinant IFN-␥ corrects these neutrophil defects. IL-12 and the more recently described IL-18 are also Th-1 type cytokines that are able to induce the production of IFN-␥ in the presence of bacteria and bacterial products. We examine the ability of GBS to induce the production of IFN-␥, IL-18, and IL-12 by cord blood mixed mononuclear cells and compared these results with the IFN-␥, IL-18, and IL-12 response of mixed mononuclear cells from adult blood. We demonstrate that cord blood mixed mononuclear cells produced significantly less IL-18 is a recently described member of the IL-1 cytokine family, which was initially defined as IFN-␥-inducing factor (1). IL-18 gene expression and/or protein secretion has been observed in macrophages (2), dendritic cells (3), mononuclear cells (4), keratinocytes (5), chondrocytes (6), pituitary and adrenal cells (7), astrocytes and microglia (8), and intestinal epithelial cells (9). Studies have elucidated a broad array of effector functions implicating IL-18 as an important regulator of both innate and acquired immune responses (10, 11). In animals, IL-18 contributes to protective immunity against a variety of pathogens, including Cryptococcus, Leishmania, Staphylococcus, Salmonella,.IL-12 is also an integral immune regulator, which promotes Th-1 responses while suppressing Th-2 responses (16,17). IL-12 is primarily produced by macrophages and dendritic cells and has been shown to induce the production of IFN-␥ by T cells and natural killer (NK) cells (18). Recent studies have focused on the interaction between IL-18 and IL-12 in certain inflammatory responses. In Th-1 immune responses, IL-18 and IL-12 are important cytokines that may synergistically stimulate IFN-␥ production and enhance NK and T cell-mediated cytotoxicity (19). Recent studies implicate the interaction of these Th-1 cytokines in the development of autoimmune diseases and suggest that regulating their function may be therapeutically beneficial (20,21).Early-onset GBS infections in neonates often lead to sepsis and severe septic shock, with an approximate 5-15% mortality rate (22)(23)(24)

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Neonatal group B streptococcal disease in Finland: a ten-year nationwide study

Cited by 91 publications

References 34 publications

Serotype Identification of Group B Streptococci by PCR and Sequencing

Serotype Identification of Group B Streptococci by PCR and Sequencing

Serotipos y susceptibilidad antimicrobiana de Streptococcus agalactiae

Defective Production of IL-18 and IL-12 by Cord Blood Mononuclear Cells Influences the T Helper-1 Interferon Gamma Response to Group B Streptococci

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