Neonatal Intermittent Hypoxia, Reactive Oxygen Species, and Oxygen-Induced Retinopathy

Beharry, Kay D.; Cai, Charles L.; Valencia, Gloria; Valencia, Arwin M.; Lazzaro, Douglas R.; Bany-Mohammed, Fayez; Aranda, Jacob V.

doi:10.20455/ros.2017.805

Cited by 12 publications

(18 citation statements)

References 141 publications

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“…On the other hand, in the early stages of life after birth, there is an exponential increase in reactive oxygen species (ROS) [ 35 ], which may be responsible for serious alterations that are very well described in the human neonate [ 36 , 37 ], calves [ 38 ] and canine newborns [ 35 ]. Among the components of colostrum, there are different essential antioxidants against oxidative damage [ 38 , 39 ]; however, the antioxidant potential of CCM exosomes has not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

et al. 2020

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Background Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

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Section: Introductionmentioning

confidence: 99%

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

et al. 2020

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“…In addition, nitric oxide (NO) may combine with oxygen free radicals, especially the superoxide anion, to form peroxynitrite (ONOO − ), a reactive nitrogen species (RNS). ROS and RNS rapidly oxidize nucleic acids, lipids, and proteins, thereby altering their structure and function and modifying the normal cellular redox status, leading to increased OS [7] (Figure 1).…”

Section: Aerobic Metabolism Reactive Oxygen Species and Oxidativmentioning

confidence: 99%

Oxidative Stress in the Newborn Period: Useful Biomarkers in the Clinical Setting

Millán¹,

Piñero-Ramos²,

Lara³

et al. 2018

Antioxidants

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Aerobic metabolism is highly efficient in providing energy for multicellular organisms. However, even under physiological conditions, an incomplete reduction of oxygen produces reactive oxygen species and, subsequently, oxidative stress. Some of these chemical species are highly reactive free radicals capable of causing functional and structural damage to cell components (protein, lipids, or nucleotides). Oxygen is the most used drug in ill-adapted patients during the newborn period. The use of oxygen may cause oxidative stress-related diseases that increase mortality and cause morbidity with adverse long-term outcomes. Conditions such as prematurity or birth asphyxia are frequently treated with oxygen supplementation. Both pathophysiological situations of hypoxia–reoxygenation in asphyxia and hyperoxia in premature infants cause a burst of reactive oxygen species and oxidative stress. Recently developed analytical assays using mass spectrometry have allowed us to determine highly specific biomarkers with minimal samples. The detection of these metabolites will help improve the diagnosis, evolution, and response to therapy in oxidative stress-related conditions during the newborn period.

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“…The steroid hormone estrogen regulates many functionally unrelated processes in numerous tissues, and exogenous estrogen and some estrogen receptor agonists have been reported to be effective in promoting retinal vascular growth and treating ROP by our and other studies. [ 5–8 ] Our previous study indicated that treatment with 17β‐E2 (an estrogen receptor agonist) could reduce oxidative stress, an important regulator in the pathogenesis of ROP, [ 9 ] in the hyperoxic phase of oxygen‐induced retinopathy (OIR). [ 6 ] G‐protein‐coupled estrogen receptor (GPER/GPR30) is uniquely localized to the endoplasmic reticulum (ER), where it specifically binds estrogen to regulate the function of ER, contributing to normal estrogen physiology as well as pathophysiology.…”

Section: Introductionmentioning

confidence: 99%

Inhibiting endoplasmic reticulum stress by activation of G‐protein‐coupled estrogen receptor to protect retinal astrocytes under hyperoxia

Wang

Chen

et al. 2020

J Biochem & Molecular Tox

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Retinal vascularization is arrested at the early (hyperoxia) stage in retinopathy of prematurity (ROP), a leading cause of blindness in children. Estrogen was reported to alleviate ROP by inhibiting reactive oxygen species, the upstream signaling molecules of endoplasmic reticulum stress (ERS). Astrocytes have long been proposed to guide angiogenesis, because they form a reticular network that provides a substrate for migrating endothelial cells. However, the factors that control the vascularization of the immature retina and the therapeutic mechanism of estrogen in early ROP remain poorly understood. This study aimed to investigate the role of G‐protein‐coupled estrogen receptor (GPER), an estrogen receptor distributed in the endoplasmic reticulum (ER), in protecting retinal astrocytes under hyperoxia and the association with ERS. The results showed that GPER was widely expressed in retinal astrocytes. GPER activation increases cell viability, decreases apoptosis, and autophagy of retinal astrocytes, decreases inositol‐1,4,5‐triphosphate receptor activity, and increases Ca2+ concentration in ER of astrocytes under hyperoxia. GPER blockade reversed all of these changes. Together, our findings indicate that GPER can protect the survival of retinal astrocytes by inhibiting ERS under hyperoxia.

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Neonatal Intermittent Hypoxia, Reactive Oxygen Species, and Oxygen-Induced Retinopathy

Cited by 12 publications

References 141 publications

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

Oxidative Stress in the Newborn Period: Useful Biomarkers in the Clinical Setting

Inhibiting endoplasmic reticulum stress by activation of G‐protein‐coupled estrogen receptor to protect retinal astrocytes under hyperoxia

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