Neonatal programming of skeletal development in sheep is mediated by somatotrophic axis function

Tatara, Marcin R.

doi:10.1113/expphysiol.2007.041145

Cited by 38 publications

(37 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been demonstrated that prenatal administration of HMB has a positive impact on the growth and development of farm animals (26,27). Until now there were no analyses concerning the influence of a prenatal administration of the HMB to pregnant dams on the morphology and functioning of the brains of their offspring.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2017

Medycyna Weterynaryjna

View full text Add to dashboard Cite

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring Summary The aim of the study was to investigate the morphology, density and immunostaining intensity of calbindin (CB)-positive neurons of dentate gyrus (DG) in new-born (P0) and 21-day-old (P21) male Acomys cahirinus mice from dams receiving β-hydroxy-β-methylbutyrate (HMB) during pregnancy. Different substances administrated to pregnant dams may affect the calcium ion homeostasis which is crucial for the proper brain development of their offspring. DG with hilus (H) plays an important role in memory and learning processes. Calcium levels in DG are regulated by buffering proteins like calbindin D28k (CB). Experimental dams were orally treated with HMB at a dose of 0.2 g/kg b.w. Half of new-born animals were euthanised after birth and the rest after the 21 st day of life. The brains were dissected and embedded in paraffin blocks using a routine histological technique. In order to demonstrate CB protein expression an immunohistochemical peroxidase-antiperoxidase reaction was conducted. The results of the study did not reveal important morphological alterations. There were no statistically significant changes in the density of the studied cells either in P0 and P21 animals. However, the authors have demonstrated a statistically significant increase of the average CB-immunostaining intensity in nuclei and cytoplasm in both age groups. It may be a result of a compensation effect to alterations that occurred under the influence of HMB. On the basis of the conducted research, it may be assumed that HMB activity in DG may provide long-term consequences.

show abstract

Section: Praca Oryginalnamentioning

confidence: 99%

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2017

Medycyna Weterynaryjna

View full text Add to dashboard Cite

show abstract

“…Urine can be obtained using a special external urine collector (Windhagen et al 2002) or collected by cystocentesis (Allen et al 2000). Urine and serum samples should be stored at -20°C for mineral analyses (Chanetsa et al 2000, Taylor et al 2009, Sousa et al 2014a) and at -80°C until determination of BTMs (Seebeck et al 2005, Tatara 2008, Sousa et al 2014b, which provides molecular stability for several months (Lomeo and Bolner 2000).…”

Section: Sample and Storagementioning

confidence: 99%

Bone turnover markers in sheep and goat: A review of the scientific literature

Camassa

Diogo

Sousa

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Bone turnover markers (BTMs) are product of bone cell activity and are generally divided in bone formation and bone resorption markers. The purpose of this review was to structure the available information on the use of BTMs in studies on small ruminants, especially for monitoring their variations related to diet, exercise, gestation and metabolic lactation state, circadian and seasonal variations, and also during skeletal growth. Pre-clinical and translational studies using BTMs with sheep and goats as animal models in orthopaedic research studies to help in the evaluation of the fracture healing process and osteoporosis research are also described in this review. The available information from the reviewed studies was systematically organized in order to highlight the most promising BTMs in small ruminant research, as well as provide a wide view of the use of sheep and goat as animal models in orthopaedic research, type of markers and commercial assay kits with cross-reactivity in sheep and goat, method of sample and storage of serum and urine for bone turnover markers determination and the usefulness and limitations of bone turnover markers in the different studies, therefore an effective tool for researchers that seek answers to different questions while using BTMs in small ruminants.

show abstract

“…Sheep are a common animal model used to study the relationship of regulatory processes of systemic growth and development with physiological, metabolic and nutritional factors, both in prenatal and postnatal life (Masters et al 1993;Blum and Hammon 2000;Kwon et al 2003;Tatara 2008). However, little is known about physiological changes in plasma free amino acid concentrations as well as their relationships during postnatal life and after the development of the forestomach.…”

Section: Discussionmentioning

confidence: 99%

“…The obtained results correspond with other studies concerning BCAA (Shimomura et al 2004). In studies on turkeys and pigs, where dietary alteration of amino acid metabolism was induced, plasma concentrations of valine, leucine and isoleucine were altered analogously in response to the applied nutritional manipulations (Tatara et al 2006(Tatara et al , 2008Tatara 2009). The balance between BCAA and aromatic amino acids such as phenylala-nine and tyrosine is known as Fischer's ratio, and is routinely utilised as an important diagnostic marker for monitoring health status and for studying the progression of liver diseases which influence amino acid metabolism (Noguchi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Changes of amino acid concentrations in Polish Merino sheep between 21 and 150 days of life

Tatara¹,

Brodzki²,

Pasternak³

et al. 2014

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of this study was to evaluate plasma amino acid profiles and their interrelationships in male Polish Merino sheep at two different stages of their systemic development -at the neonatal monogastric stage and as a fully grown ruminant. Male lambs (n = 12, singles) were kept indoors in pens under standard rearing conditions and weaned at the age of 10 weeks of life. Blood samples were collected on day 21 and 150 of life. Free amino acids were determined in plasma using ion-exchange chromatography. There was a significant reduction in plasma amino acid concentrations between neonatal and functioning ruminants for threonine, serine, glutamate, proline, citrulline, tyrosine, tryptophan, lysine, histidine and arginine. Significant increases in the plasma concentrations of alpha-amino-butyrate and isoleucine were observed in 150 day old lambs. Except for glutamine in 21 day old sheep which was significantly negatively correlated with serine, glutamate, proline and tyrosine concentrations in plasma, the obtained results in younger and older groups of lambs showed 47 and 22 significant positive correlations between the evaluated amino acids. This study shows that decreases in plasma amino acid concentrations in 150 day old sheep with physiologically developed forestomachs are dominant in comparison to 21 day-old animals receiving maternal milk as a basic diet. Our results address the possibility of utilising improved amino acid supply for growing lambs with developed forestomachs.

show abstract

Neonatal programming of skeletal development in sheep is mediated by somatotrophic axis function

Cited by 38 publications

References 41 publications

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Influence of oral administration of HMB to pregnant dams on calbindin expression in the dentate gyrus of the hippocampus during postnatal development in spiny mice offspring

Bone turnover markers in sheep and goat: A review of the scientific literature

Changes of amino acid concentrations in Polish Merino sheep between 21 and 150 days of life

Contact Info

Product

Resources

About