Neonatal small bowel epithelia: enhancing anti-bacterial defense with lactoferrin and Lactobacillus GG

Sherman, Michael P.; Bennett, Stephen H.; Hwang, F; Yu, Camille

doi:10.1023/b:biom.0000027706.51112.62

Cited by 107 publications

(74 citation statements)

References 15 publications

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“…Components of AF that may promote these effects include glutamine, 55 arginine, 56 EGF, 57 EPO, 58 PAF-AH, 59 and LF. 60 Could harvested or synthetic AF be used as an enteral infusion in the preterm neonate at risk for or recovering from NEC? Would scarring of the gut be decreased?…”

Section: Unanswered Questions and Future Directions For Af-related Rementioning

confidence: 99%

Amniotic Fluid: Not Just Fetal Urine Anymore

2005

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Section: Unanswered Questions and Future Directions For Af-related Rementioning

confidence: 99%

Amniotic Fluid: Not Just Fetal Urine Anymore

2005

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“…In particular, lactoferrin may support the growth of some nonpathological but beneficial bacteria [14,15]. …”

Section: Putative Lysines Are Represented As Green Spheres At the Ne mentioning

confidence: 99%

“…Lactoferrin can also serve as an iron donor, supporting the growth of certain beneficial bacteria [14,15]. This occurs in breast milk, where lactoferrin,…”

Section: Introductionmentioning

confidence: 99%

Monitoring lactoferrin iron levels by fluorescence resonance energy transfer: a combined chemical and computational study

et al. 2014

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Abstract.Three forms of lactoferrin (Lf) that differed in their levels of iron loading (Lf, LfFe, and LfFe 2 ) were simultaneously labeled with the fluorophores AF350 and AF430. All three resulting fluorescent lactoferrins exhibited fluorescence resonance energy transfer (FRET), but they all presented different FRET patterns. Whereas only partial FRET was observed for Lf and LfFe, practically complete FRET was seen for the holo form (LfFe 2 ). For each form of metal-loaded lactoferrin, the AF350-AF430 distance varied depending on the protein conformation, which in turn depended on the level of iron loading. Thus, the FRET patterns of these lactoferrins were found to correlate with their iron loading levels. In order to gain greater insight into the number of fluorophores and the different FRET patterns observed (i.e., their iron levels), a computational analysis was performed. The results highlighted a number of lysines that have the greatest influence on the FRET profile. Moreover, despite the lack of an X-ray structure for any LfFe species, our study also showed that this species presents modified subdomain organization of the N-lobe, which narrows its iron-binding site. Complete domain rearrangement occurs during the LfFe to LfFe 2 transition. This latter study demonstrated that lactoferrin supplies iron to this bacterium, and suggested that this process occurs with no protein internalization.

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“…In contrast, lactoferrin may serve as iron donor, and in this manner support the growth of some bacteria with lower iron demands such as Lactobacillus sp. or Bifidobacterium sp., generally considered as beneficial (Petschow et al, 1999;Sherman et al, 2004). Nevertheless, some bacteria are able to adapt to the new conditions and release siderophores (iron chelating compounds of bacterial origin) that compete with lactoferrin for Fe 3+ ions (Crosa, 1989;Ratledge and Dover, 2000).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Lactoferrin: a review

Adlerova¹,

Bartošková²,

Faldyna³

2008

Vet. Med.

267

217

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This review discusses the biological properties of the glycoprotein lactoferrin. Lactoferrin has been identified in secretions from exocrine glands and in specific granules of neutrophils. After degranulation, neutrophils become the main source of lactoferrin in blood plasma. Lactoferrin possesses various biological functions, including roles in iron metabolism, cell proliferation and differentiation, and antibacterial, antiviral, and antiparasitic activity. Many of these functions do not appear to be connected with its iron binding ability. Of late, lactoferrin concentrations have been measured mostly in humans but also in some other species. However, the relationship between its concentration and physiological or pathological effects on body functions is not yet well characterised.

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Neonatal small bowel epithelia: enhancing anti-bacterial defense with lactoferrin and Lactobacillus GG

Cited by 107 publications

References 15 publications

Amniotic Fluid: Not Just Fetal Urine Anymore

Amniotic Fluid: Not Just Fetal Urine Anymore

Monitoring lactoferrin iron levels by fluorescence resonance energy transfer: a combined chemical and computational study

Lactoferrin: a review

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