Neonatal Sublingual Vaccination with <i>Salmonella</i> Proteins and Adjuvant Cholera Toxin or CpG Oligodeoxynucleotides Induces Mucosal and Systemic Immunity in Mice

Huang, Ching-Feng; Wang, Chih‐Chien; Wu, Tzee–Chung; Wu, Keh-Gong; Lee, Chin-Cheng; Peng, Ho-Jen

doi:10.1097/mpg.0b013e318156050d

Cited by 20 publications

(20 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For the first time, we demonstrate the development of both high T h 1 and T h 17 function in the neonatal intestine to a fully virulent infectious bacterium in the absence of additional interventions. Based on the current understanding that immune responsiveness during the neonatal period of life can be modulated extensively by targeting the mucosal immune system (1,8,11,12,27,29,34,35,59,70) and by using strong immunomodulatory agents (31,36,51), we favor the idea that robust immunity to Y. enterocolitica is a function of both the inflammatory potential of this pathogen and an intrinsic capacity of the neonatal intestine to develop high-level inflammation. These data support the idea that the development of inflammatory CD4…”

Section: Discussionmentioning

confidence: 99%

“…Altogether, it has become apparent that under the proper stimulation conditions, all arms of the neonatal adaptive immune system can be induced to generate adult-like responses. Importantly, some of these immunization regimens promoted protective immunity against infection with fully pathogenic bacteria (16,29,31,34,42,59). …”

mentioning

confidence: 99%

“…For example, bacterially derived products such as mutated Escherichia coli enterotoxins LT-R192G (70) and LT-K63 (11,14,27,34), CpG oligonucleotides (CpG) (8,29,31), and lyophilized bacterial extracts (12) have been described to markedly enhance neonatal vaccine responses. Another approach used to improve immune responses has been the delivery of specific antigens using live attenuated bacterial vectors such as Listeria monocytogenes (42,50) and Salmonella species (16,59).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Yersinia enterocoliticaPromotes Robust Mucosal Inflammatory T-Cell Immunity in Murine Neonates

et al. 2010

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Yersinia enterocoliticaPromotes Robust Mucosal Inflammatory T-Cell Immunity in Murine Neonates

et al. 2010

View full text Add to dashboard Cite

“…Specific IgG antibodies were assessed using ELISA as described [31]. Briefly, plates were coated with 4 µg/ml rCyn d 1 in 0.05 M carbonate buffer, pH 9.6 (100 µl/well), overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Induction of Specific Th1 Responses and Suppression of IgE Antibody Formation by Vaccination with Plasmid DNA Encoding Cyn d 1

Huang

Chu

et al. 2012

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: DNA vaccines encoding allergens have been developed to prevent or to treat specific IgE responses. Objective: To evaluate the potential preventive and therapeutic effect of DNA vaccines encoding Cyn d 1 alone or combined with different adjuvants on specific allergies. Methods: Recombinant plasmid Cyn d 1 (pCyn d 1) was constructed by insertion of Cyn d 1 cDNA into the vector pcDNA3. BALB/c mice were injected with pCyn d 1 alone or plus adjuvants such as bupivacaine, bestatin, liposome, or CpG. Control mice were treated with pcDNA3 or PBS. They were boosted 3 weeks later and then sensitized twice with recombinant Cyn d 1 and alum. Their serum antibody responses and cytokine profiles of spleen cells were studied. Adoptive transfer of spleen cells of pCyn d 1-vaccinated mice was also performed. Results: Vaccination of mice with pCyn d 1 induced Th1 responses characterized by IgG2a responses and spleen cell secretion of interferon-γ. Vaccination with pCyn d 1 not only prevented the induction of specific IgE responses but also suppressed ongoing IgE responses. The mice receiving untreated, CD4+- or CD8+-depleted spleen cells from pCyn d 1-vaccinated mice all had suppression of IgE responses. Conclusion: This study confirms the prophylactic and therapeutic effects of DNA vaccines encoding Bermuda grass pollen allergen Cyn d 1 on specific IgE responses. Both CD4+ and CD8+ T cells are crucial for the immunomodulatory effect of pCyn d 1 on specific IgE responses.

show abstract

“…These findings have prompted exploration of alternative mucosal vaccine routes, particularly for administration of adjuvanted influenza vaccines. The sublingual (SL) route has been used for decades to treat angina [17] and has more recently been investigated for allergen desensitisation therapy [18], [19] and administration of vaccines against various bacterial and viral diseases [20], [21], [22], [23]. An adjuvanted seasonal influenza H1N1 vaccine (whole inactivated A/PR/8) has also proved effective when administered sublingually to mice [15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Sublingual Route for Administration of Influenza H5N1 Virosomes in Combination with the Bacterial Second Messenger c-di-GMP

et al. 2011

View full text Add to dashboard Cite

Avian influenza A H5N1 is a virus with pandemic potential. Mucosal vaccines are attractive as they have the potential to block viruses at the site of entry, thereby preventing both disease and further transmission. The intranasal route is safe for the administration of seasonal live-attenuated influenza vaccines, but may be less suitable for administration of pandemic vaccines. Research into novel mucosal routes is therefore needed. In this study, a murine model was used to compare sublingual administration with intranasal and intramuscular administration of influenza H5N1 virosomes (2 µg haemagglutinin; HA) in combination with the mucosal adjuvant (3′,5′)-cyclic dimeric guanylic acid (c-di-GMP). We found that sublingual immunisation effectively induced local and systemic H5N1-specific humoral and cellular immune responses but that the magnitude of response was lower than after intranasal administration. However, both the mucosal routes were superior to intramuscular immunisation for induction of local humoral and systemic cellular immune responses including high frequencies of splenic H5N1-specific multifunctional (IL-2+TNF-α+) CD4+ T cells. The c-di-GMP adjuvanted vaccine elicited systemic haemagglutination inhibition (HI) antibody responses (geometric mean titres ≥40) both when administered sublingually, intranasally and inramuscularly. In addition, salivary HI antibodies were elicited by mucosal, but not intramuscular vaccination. We conclude that the sublingual route is an attractive alternative for administration of pandemic influenza vaccines.

show abstract

Neonatal Sublingual Vaccination with Salmonella Proteins and Adjuvant Cholera Toxin or CpG Oligodeoxynucleotides Induces Mucosal and Systemic Immunity in Mice

Abstract: Neonatal sublingual vaccination with adjuvant CpG or CT can induce both mucosal and systemic immunity and may play a crucial role in protection against enteric pathogens.

Cited by 20 publications

References 39 publications

Yersinia enterocoliticaPromotes Robust Mucosal Inflammatory T-Cell Immunity in Murine Neonates

Yersinia enterocoliticaPromotes Robust Mucosal Inflammatory T-Cell Immunity in Murine Neonates

Induction of Specific Th1 Responses and Suppression of IgE Antibody Formation by Vaccination with Plasmid DNA Encoding Cyn d 1

Evaluation of the Sublingual Route for Administration of Influenza H5N1 Virosomes in Combination with the Bacterial Second Messenger c-di-GMP

Contact Info

Product

Resources

About