Neonatally wounded skin induces NGF-independent sensory neurite outgrowth in vitro

Reynolds, M. L.; Alvares, Debie; Middleton, Jacqueta; Fitzgerald, Maria

doi:10.1016/s0165-3806(97)00105-3

Cited by 33 publications

(31 citation statements)

References 35 publications

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“…The reasons that selective hyperinnervation occurs differentially in different types of wounds is unclear. However, in cultured peri-infarct tissue, NGF appears to be essential for enhanced sympathetic sprouting, whereas similar explant studies showed that sensory axon sprouting induced by wounded skin from neonates occurs largely independently of NGF (Reynolds, et al, 1997). Thus, the nature of wound hyperinnervation apparently varies with tissue type and as a function of which neurotrophic factors are produced.…”

Section: Hyperinnervation and Its Relationship To Cardiac Wound Healingmentioning

confidence: 98%

Sympathetic hyperinnervation and inflammatory cell NGF synthesis following myocardial infarction in rats

et al. 2006

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Sympathetic hyperinnervation occurs in human ventricular tissue after myocardial infarction and may contribute to arrhythmias. Aberrant sympathetic sprouting is associated with elevated nerve growth factor (NGF) in many contexts, including ventricular hyperinnervation. However, it is unclear whether cardiomyocytes or other cell types are responsible for increased NGF synthesis. In this study, left coronary arteries were ligated and ventricular tissue examined in rats 1-28 days post-infarction. Infarct and peri-infarct tissue was essentially devoid of sensory and parasympathetic nerves at all time points. However, areas of increased sympathetic nerve density were observed in the peri-infarct zone between post-ligation days 4-14. Hyperinnervation occurred in regions containing accumulations of macrophages and myofibroblasts. To assess whether these inflammatory cells synthesize NGF, sections were processed for NGF in situ hybridization and immunohistochemistry. Both macrophage1 antigen-positive macrophages and α-smooth muscle actin immunoreactive myofibroblasts expressed NGF in areas where they were closely proximate to sympathetic nerves. To investigate whether NGF produced by peri-infarct cells induces sympathetic outgrowth, we cocultured adult sympathetic ganglia with peri-infarct explants. Neurite outgrowth from sympathetic ganglia was significantly greater at post-ligation days 7-14 as compared to control tissue. Addition of an NGF function-blocking antibody prevented the increased neurite outgrowth induced by periinfarct tissue. These findings provide evidence that inflammatory cell NGF synthesis plays a causal role in sympathetic hyperinnervation following myocardial infarction.

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Section: Hyperinnervation and Its Relationship To Cardiac Wound Healingmentioning

confidence: 98%

Sympathetic hyperinnervation and inflammatory cell NGF synthesis following myocardial infarction in rats

et al. 2006

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“…Reynolds and Fitzgerald [126] reported that skin wounds placed in newborn rats (at P0, P7) caused a marked hyperinnervation and decreased thresholds in the injured area lasting longer than 3 months after the injury. Nerve sprouting from A and C fibers was markedly increased around skin wounds placed on the day of birth (P0) as compared with older ages, and was stimulated by nerve growth factor in older rats and unknown neurotrophic factors in the neonate [127]. Neonatal rats subjected to carrageenan-induced inflammation on P1 developed a persistent expansion (by approximately 30%) in the receptive field of their dorsal horn neurons [128], although the mechanisms mediating these changes remain unknown.…”

Section: Long-term Effects Of Pain In Neonatal Ratsmentioning

confidence: 99%

Can Adverse Neonatal Experiences Alter Brain Development and Subsequent Behavior?

Anand

Scalzo²

2000

Neonatology

538

316

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Self-destructive behavior in current society promotes a search for psychobiological factors underlying this epidemic. Perinatal brain plasticity increases the vulnerability to early adverse experiences, thus leading to abnormal development and behavior. Although several epidemiological investigations have correlated perinatal and neonatal complications with abnormal adult behavior, our understanding of the underlying mechanisms remains rudimentary. Models of early experience, such as repetitive pain, sepsis, or maternal separation in rodents and other species have noted multiple alterations in the adult brain, correlated with specific behavioral phenotypes depending on the timing and nature of the insult. The mechanisms mediating such changes in the neonatal brain have remained largely unexplored. We propose that lack of N-methyl-D-aspartate (NMDA) receptor activity from maternal separation and sensory isolation leads to increased apoptosis in multiple areas of the immature brain. On the other hand, exposure to repetitive pain may cause excessive NMDA/excitatory amino acid activation resulting in excitotoxic damage to developing neurons. These changes promote two distinct behavioral phenotypes characterized by increased anxiety, altered pain sensitivity, stress disorders, hyperactivity/attention deficit disorder, leading to impaired social skills and patterns of self-destructive behavior. The clinical important of these mechanisms lies in the prevention of early insults, effective treatment of neonatal pain and stress, and perhaps the discovery of novel therapeutic approaches that limit neuronal excitotoxicity or apoptosis.

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“…Most of the data are derived fiom animal models that suggest that repeated painfil stimuli result in permmsnt structural and functional reorganization of the nervous system and alteration in future pain responses (Fitzgerald & Anand, 1993;Plotslq et al, 2000;Ruda, Ling, Hohmann, Peng & Tachibana, 2000). Reynolds, Alvares, Middleton and Fitzgerald (1997) reported that skin wounds inflicted on rat pups caused hyper-innervation and decreased pain thresholds in the injured area that lasted three months afier the painfil event. Rats subjected to recurrent pain from day one of life developed changes in the receptive field of their dorsal horn neurons that persisted over time.…”

Section: Long-term Conseauences Of Painmentioning

confidence: 99%

Efficacy and Safety of Sucrose for Procedural Pain Relief in Preterm and Term Neonates

et al. 2002

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Neonatally wounded skin induces NGF-independent sensory neurite outgrowth in vitro

Cited by 33 publications

References 35 publications

Sympathetic hyperinnervation and inflammatory cell NGF synthesis following myocardial infarction in rats

Sympathetic hyperinnervation and inflammatory cell NGF synthesis following myocardial infarction in rats

Can Adverse Neonatal Experiences Alter Brain Development and Subsequent Behavior?

Efficacy and Safety of Sucrose for Procedural Pain Relief in Preterm and Term Neonates

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