Neoplasia in Zebrafish (Danio rerio) Treated with 7,12-Diniethylbenz[a]anthracene by Two Exposure Routes at Different Developmental Stages

Spitsbergen, Jan M.; Tsai, Hsi-Wen; Reddy, Ashok P.; Miller, Tom; Arbogast, Dan; Hendricks, Jerry D.; Bailey, George S.

doi:10.1177/019262330002800511

Cited by 161 publications

(125 citation statements)

References 37 publications

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“…All the gills of the DR fish (n = 9), but only about half of the gills from the QMH fish (n = 11), exhibited minor cellular changes of unknown etiology (e.g., small foci of necrosis), as well as some degree of epithelial hyperplasia of the lamellae. One of the DR fish had an apparent carcinoma of the gill epithelium, similar to other gill carcinomas that have been described (Spitsbergen et al 2000).…”

Section: Resultssupporting

confidence: 74%

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

Malins

Stegeman

Anderson

et al. 2004

Environ Health Perspect

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Structural differences were identified in gill DNA from two groups of English sole collected from Puget Sound, Washington, in October 2000. One group was from the industrialized Duwamish River (DR) in Seattle and the other from relatively clean Quartermaster Harbor (QMH). Chemical markers of sediment contamination [e.g., polynuclear aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs)] established that the DR was substantially more contaminated than QMH. The levels of these chemicals in the sediments of both sites were consistent with levels of cytochrome P450 1A (CYP1A) expression in the gills of English sole from the same sites. Structural differences in gill DNA between the groups were evinced via statistical models of Fourier transform-infrared (FT-IR) spectra. Marked structural damage was found in the gill DNA of the DR fish as reflected in differences in base functional groups (e.g., C-O and NH 2 ) and conformational properties (e.g., arising from perturbations in vertical base stacking interactions). These DNA differences were used to discriminate between the two fish groups through principal components analysis of mean FT-IR spectra. In addition, logistic regression analysis allowed for the development of a "DNA damage index" to assess the effects of contaminants on the gill. The evidence implies that environmental chemicals contribute to the DNA changes in the gill. The damaged DNA is a promising marker for identifying, through gill biopsies, contaminant effects on fish.

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Section: Resultssupporting

confidence: 74%

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

Malins

Stegeman

Anderson

et al. 2004

Environ Health Perspect

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“…These models mainly include three different types. The first type is that Spitsbergen et al (23,24) gained by chemical induction and acquiring several types of cancers in zebrafish through chemical carcinogenesis such as epithelial, mesenchymal, neural neoplasia induced by 7,12-dimethylbenz[a]anthracene and hepatic, mesenchymal neoplasia induced by N-methyl-N'-nitro-N-nitrosoguanidine. The second type of zebrafish cancer model is obtained by genetic manipulation, bmyb mutation causes genome instability and increased cancer susceptibility in zebrafish (25).…”

Section: Discussionmentioning

confidence: 99%

VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells

Wang

et al. 2012

Oncology Reports

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Abstract. Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches. IntroductionGlioma is the most common primary brain tumor in adults and the median survival for patients without therapy is up to 3 months (1). Currently the traditional treatment such as resection, chemotherapy and radiotherapy has not greatly improved the median survival for patients. Malignant glioma is notorious for its behavior of rapid proliferation, great invasion and metastasis and it is also among the best vascularized tumors in humans. It is well known that angiogenesis plays a critical role in tumor progression. Therefore new anti-angiogenic treatment strategies are required (2-6). Revealing of various signaling pathways that lead to activation of angiogenesis will provide molecular insight into developing therapeutic agents to treat glioma. In the past decades, researchers have discovered many molecular mechanisms related to tumor angiogenesis (7-9).Traditional rodent cancer models have disadvantages, e.g., the high cost, long developmental phase, and limited availability for high-throughput assays (10), which limited effective ways to find the new molecular mechanisms and monitor the tumor in vivo in real time.The teleost zebrafish (Danio rerio), a promising alternative vertebrate cancer model has attracted considerable attention in recent years because of compelling advantages of transparency of the embryo, rapid development, fecundity, tractable genetics, great screening efficiency, high levels of physiologic and genetic homology with higher vertebrates (11-13). Moreover, the zebrafish embryo within 1 week is immune-free, and showed no immunosuppression to transplanted human glioma cells (14). The transgenic zebrafish strains, e.g., casper transparent mutant line (15), and VEGFR2:G-RCFP line with green fluorescence specifically in blood vessels (16), offered new tools for cancer research.In this study, we microinjected human U87 glioma cells into zebrafish embryos and developed a xenograft zebrafish glioma model. The U87 cells are first labeled with a red fluorescence protein and then microinjected into perivitelline space of VEGFR2:G-RCFP transgenic zebrafish embryos at 48 h post-fertilization. Then in order to investigate the angiogenesis mechanisms in details, we make use of the staining of endogenous alkaline phosphatase, fluorescent microscopy monitoring, in situ hybridization and quantitive RT-PCR to investigate the ch...

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“…More recently, similar but more extensive studies showed that N-nitrosodiethylamine primarily induces liver and pancreas carcinomas (8), and N-nitrosodimethylamine induces only liver tumors (9). 7,12-Dimethylbenz[a]anthracene induces the broadest tumor spectrum, including liver neoplasms; epithelial tumors in intestine, pancreas, thyroid, and testis; mesenchymal tumors in cartilage, blood vessels, muscles, and connective and lymphoid tissues; and neural tumors (10). N-Methyl-N ¶-nitro-N-nitrosoguanidine can also induce different tumor types, mainly liver and testicular neoplasms, as well as hemangio(sarco)mas and others (11).…”

Section: Treatment With Mutagensmentioning

confidence: 97%

“…In any case, the frequency of cancer in mutant fish is much higher than in wild types, which spontaneously develop tumors in the order of 0% (27) to 11% (24) of the population. Another long-term study of spontaneous neoplasms determined that wild-type fish have 1% cancer incidence in the first year, which increases in the second year (10). However, most groups agree that more data need to be generated on tumor incidence in wild-type fish to improve the model.…”

Section: Cancer Incidence and Onsetmentioning

confidence: 99%

Zebrafish as a Cancer Model

Feitsma

Cuppen

2008

Molecular Cancer Research

232

155

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Neoplasia in Zebrafish (Danio rerio) Treated with 7,12-Diniethylbenz[a]anthracene by Two Exposure Routes at Different Developmental Stages

Abstract: Using zebrafish, Danio

Cited by 161 publications

References 37 publications

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

Structural changes in gill DNA reveal the effects of contaminants on Puget Sound fish.

VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells

Zebrafish as a Cancer Model

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