Neospora caninum: Seroprevalence and DNA detection in blood of sheep from Aguascalientes, Mexico

Castañeda-Hernández, A.; Cruz-Vázquez, Carlos; Medina-Esparza, Leticia

doi:10.1016/j.smallrumres.2014.03.002

Cited by 13 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A very similar—if not identical—route of transmission has been described previously in bovine neosporosis, where it has been suggested that during reactivation of a latent infection in pregnant animals (i.e., recrudescence), encysted N. caninum bradyzoites located in tissue cysts differentiate into tachyzoites, and subsequently disseminate throughout the host via the bloodstream (i.e., parasitemia), allowing invasion of the placenta and infection of the fetus [ 22 ]. This theory is supported by parasite DNA having been found in blood samples from chronically infected sheep in epidemiological studies [ 7 , 23 ], as it has in cattle [ 24 , 25 ].…”

Section: Life Cycle and Transmissionmentioning

confidence: 87%

Ovine Neosporosis: The Current Global Situation

Benavides

González-Warleta

Arteche-Villasol

et al. 2022

Animals

View full text Add to dashboard Cite

In the past 20 years, Neospora caninum infection in sheep has been reported in at least 31 countries worldwide from all sheep-rearing continents (Europe, Asia, the Americas, Africa, and Oceania), and its role as an abortifacient agent is becoming more evident. Most studies of ovine neosporosis have focused on its epidemiology, based primarily on serological analysis, with only a few studies investigating the actual presence of the parasite by PCR and/or IHC. Individual seroprevalence rates were highly variable between countries, and even between regions within the same country, ranging from 0.0% to 67.4% positive. Furthermore, most of the studies were not directly comparable due to differences in experimental designs, sample sizes, husbandry systems, ecological factors, and serological tests (e.g., IFAT, ELISA, MAT, Western blot). The latter, along with the scarcity of studies on the relevance of N. caninum as an abortifacient agent, may bias the perception of the importance of this disease. This review summarizes the situation of N. caninum infection in sheep using all available published studies describing natural ovine neosporosis. The epidemiology shows that ovine neosporosis is found worldwide, and it poses a relevant risk to the sustainability of sheep flocks.

show abstract

Section: Life Cycle and Transmissionmentioning

confidence: 87%

Ovine Neosporosis: The Current Global Situation

Benavides

González-Warleta

Arteche-Villasol

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…La concordancia entre la prueba de ELISA y la de PCR en sangre fue k= 0.36, considerada mediana; en otros estudios este valor ha tendido a ser de pobre a moderado (21,23,24) . En la literatura se ha documentado que la circulación de taquizoitos de N. caninum en sangre periférica es pulsatil (25,26) , por lo que esta prueba debe de complementarse con alguna otra de tipo serológico.…”

Section: Discussionunclassified

Detección de Neospora caninum en ganado lechero sacrificado en Aguascalientes, México

Esparza

Oseguera

Mendoza

et al. 2018

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

Con el objetivo de detectar la presencia de Neospora caninum en tejidos, suero y sangre periférica de ganado lechero sacrificado en rastros de Aguascalientes, México, se colectaron muestras de médula espinal, corazón, hígado, sangre y suero sanguíneo de 71 animales adultos. Las muestras de suero se procesaron mediante la prueba de ELISA para detectar anticuerpos anti-N. caninum, mientras que las otras se sometieron a la prueba de PCR anidado para determinar la frecuencia de ADN del parásito en los diferentes tejidos, así como en sangre. La frecuencia general de animales seropositivos fue de 41 % (29/71; IC 95% 29-53) con un rango de 33 a 57 %. La frecuencia de detección de ADN de N. caninum en sangre fue 44 % (31/71; IC 95% 32-55), con un rango entre 38 y 50 %; en las muestras de médula espinal de 32 % (27/71; IC 95% 27-50), con un rango de 2 a 38 %; en las muestras de corazón, 22 % (16/71; IC 95% 13-34), con un rango de 4 a 33 %; y en las de hígado, 32 % (23/71; IC 95% 22-44), con un rango de 28 a 57 %. La concordancia entre la prueba de ELISA y la de PCR en sangre fue k= 0.36, de ELISA y PCR en tejidos k= 0.07 y de PCR en sangre y PCR en tejidos k= 0.61. Únicamente 11 vacas fueron positivas a las tres pruebas.

show abstract

“…It has previously been shown that N. caninum DNA can be amplified from the blood of infected aborted and pregnant cows [ 17 , 48 ], bulls [ 49 ], mice [ 50 ] and sheep [ 51 , 52 ]. Ghalmi et al [ 47 ] also showed that circulating parasites or parasite DNA could be detected in the blood of naturally infected dogs.…”

Section: Discussionmentioning

confidence: 99%

“…However, care must be taken in making generalisations about N. caninum based on the biology of T. gondii because neosporosis and toxoplasmosis are biologically distinct diseases [ 2 ]. Molecular assays are useful for early detection of N. caninum in asymptomatic animals [ 7 , 17 , 47 , 52 ] because blood seems to provide the transport medium for tachyzoites between body tissues [ 48 ]. However, establishing an epidemiological relevance or cause-effect relationship between abortion and N. caninum is difficult because it was not known that detected DNA derived from multiplying tachyzoites in blood or free floating DNA, and finding the presence of the parasite DNA does not mean that N. caninum caused the abortion [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis

et al. 2017

View full text Add to dashboard Cite

BackgroundDogs are the definitive hosts of Neospora caninum and play an important role in the transmission of the parasite. Despite the high sensitivity of existing molecular tools such as quantitative real-time PCR (qPCR), these techniques are not suitable for use in many countries because of equipment costs and difficulties in implementing them for field diagnostics. Therefore, we developed a simplified technique, loop-mediated isothermal amplification (LAMP), for the rapid visual detection of N. caninum.MethodsLAMP specificity was evaluated using a panel containing DNA from a range of different organisms. Sensitivity was evaluated by preparing 10-fold serial dilutions of N. caninum tachyzoites and comparing the results with those obtained using qPCR. Assessment of the LAMP results was determined by recognition of a colour change after amplification. The usefulness of the LAMP assay in the field was tested on 396 blood and 115 faecal samples from dogs, and one placenta from a heifer collected in Lopburi, Nakhon Pathom, Sa Kaeo, and Ratchaburi provinces, Thailand.ResultsSpecificity of the LAMP technique was shown by its inability to amplify DNA from non-target pathogens or healthy dogs. The detection limit was the equivalent of one genome for both LAMP and qPCR. LAMP and qPCR detected positive N. caninum infection in 15 of 396 (3.8%) blood samples; LAMP detected 9/115 (7.8%) positive faecal samples, while qPCR detected 5/115 (4.3%) positive faecal samples. The placental tissue was shown to be positive by both techniques. Agreement between LAMP and qPCR was perfect in blood samples (kappa value, 1.00) and substantial in faecal samples (kappa value, 0.697).ConclusionsThis is the first known LAMP assay developed for the amplification of N. caninum. The technique effectively and rapidly detected the parasite with high sensitivity and specificity and was cost-effective. This assay could be used in the field to confirm the diagnosis of canine or bovine neosporosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2330-2) contains supplementary material, which is available to authorized users.

show abstract

Neospora caninum: Seroprevalence and DNA detection in blood of sheep from Aguascalientes, Mexico

Cited by 13 publications

References 37 publications

Ovine Neosporosis: The Current Global Situation

Ovine Neosporosis: The Current Global Situation

Detección de Neospora caninum en ganado lechero sacrificado en Aguascalientes, México

Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis

Contact Info

Product

Resources

About