Neovascularization: topical effects of streptococcus thermophilus and low level laser therapy in treatment of diabetic wound in rats

Abstract

Background: Diabetic wound is a major socioeconomic debilitating problem in this society. Various treatment options are available but still it requires better treatment option. In diabetes mellitus the oxygenation to the tissues is reduced. In this study effects of low level laser therapy were compared with topical application of Streptococcus thermophilus on diabetic wounds that induces formation of new blood vessel and free radical scavenging system, a comparative study to get better treatment option for diabetic wounds.Methods: 18 male rats were selected and divided randomly into three groups. Diabetes was induced in all the rats by using the Alloxan monohydrate at a dose of 120mg/kg of the body weight. Group A was treated with normal saline, group B was treated with low level laser therapy and group C was treated with Streptococcus thermophilus topically. Skin tissues were collected on day three and seven, slides were prepared for microscopic examination to observe the new blood vessels formation.Results: Mean number of new blood vessel formation was observed in group B compared with group A and C. Significant vasculogenesis was seen in group B when treated with Low level laser therapy.Conclusions: In the group of low level laser therapy new blood vessel formation was seen with better wound healing. It means LLLT provides better oxygenation to the tissues by generation of new blood vessels compared with Streptococcus thermophilus and normal saline.

Gut Dysbiosis and Diabetic Foot Ulcer: Role of Probiotics

“…However, it was observed that when Streptococcus thermophilus treatment and low-level laser therapy were used in combination, accelerated DW healing occurred. In addition, the combination promoted angiogenesis and collagen deposition at the site of injury [47].…”

“…In another study, Kumari et al (2019) examined the effect of Streptococcus thermophilus and low-level laser therapy on DW healing in male Albino diabetic rats. The results revealed that the topical application of saline did not lead to effective wound contraction while Streptococcus thermophilus showed a reduction in oxidative stress and promoted DW healing.…”

Effect of Low-level Light Therapy on Post-operative Healing of Secondary Chronic Osteomyelitis of the Jaws - A Prospective Study

“…Aside from osteomyelitis research, the effect of LLLT on bone repair was thoroughly carried out and showed stimulation of bone production;[ 30 31 32 ] therefore, the positive effect of this treatment modality can be postulated. A stimulating effect was recorded in terms of blood and lymph neovascularisation,[ 33 34 ] synthesis of RNA, DNA, collagen and its precursors, as well as positive effects on the levels of prostaglandin, phagocyte cytoplasmic granules, cell proliferation and production of adenosine-5’- triphosphate. [ 35 36 ] Furthermore, an existent penetration of LED 635-nm low-level light through the soft and hard tissues of the jaws was demonstrated[ 37 ] which implies that the complete jaw bone thickness receives therapeutic doses.…”