Nephroprotective effect of apilarnil in lipopolysaccharide-induced sepsis through TLR4/NF-κB signaling pathway

Doğanyiğit, Züleyha; Okan, Aslı; Kaymak, Emin; Silici, Sibel

doi:10.1016/j.lfs.2021.119875

Cited by 17 publications

(17 citation statements)

References 44 publications

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“…After the release of cytokines, the coagulation systems are stimulated. Although these cytokines are beneficial in diminishing local infection, they cause endothelial damage as a result of their oversynthesis (Inandiklioglu et al, 2021). Studies in the literature demonstrate that IL-1β and TNF-α levels are significantly increased in kidney damage due to sepsis caused by LPS studies.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Studies in the literature demonstrate that IL‐1β and TNF‐α levels are significantly increased in kidney damage due to sepsis caused by LPS studies. It has been described in studies that IL‐6 and TNF‐α play a role in the septic action and levels increase particularly in endotoxic shock and increase the death rate (Cross & Opal, 2003; Inandiklioglu et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…TLR4 is a receptor that recruits inflammatory factors and subsequently causes kidney and liver damage, and by increasing the level of TLR4, it stimulates the expression of proinflammatory cytokines IL‐6 and TNF‐α, thereby activating the NF‐kB pathway. Studies have reported that increased NF‐kB activation is tightly involved to organ damage (Inandiklioglu et al, 2021; Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…With the most dangerous form, sepsis is a condition characterized by severe immune system dysfunction causing multi-organ dysfunction. Much has been reported about the pathogenesis of sepsis at the molecular, cellular and intact organ level (Inandiklioglu et al, 2021;Purcarea & Sovaila, 2020). It is reported that nearly 1400 sufferer die daily and 500,000 sufferer die yearly due to sepsis in the world (Angus et al, 2001).Grave sepsis states are defined as sepsis associated with organ failure, hypotension, or hyperfusion.…”

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confidence: 99%

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Detection of melatonin protective effects in sepsis via argyrophilic nucleolar regulatory region‐associated protein synthesis and TLR4/NF‐κB signaling pathway

et al. 2022

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In this study, the protective effect of melatonin was investigated in lipopolysaccharide induced sepsis model. Twenty‐eight rats were randomly divided: Control, Melatonin, LPS and LPS + Melatonin. After LPS application, surgically remove kidney and liver tissues. The level of malondialdehyde (MDA) an oxidative stress marker and the immunoreactivity of Toll‐like receptor‐4 (TLR4), tumor necrosis factor‐α (TNF‐α), and transcription factor NF‐κB were evaluated immunohistochemically. Expression levels for TLR4, TNF‐α, NF‐kB, IL‐1β (interleukin 1 beta), and IL‐6 (interleukin 6) were evaluated. Additionally, Argyrophilic NOR staining was performed in tissues. Vacuolization and inflammation were more intense in the kidney and liver sections in the LPS group compared to the other groups. It was observed that vacuolization and inflammation were decreased in LPS + Melatonin applied groups. It was determined that glomerular damage was increased in the LPS and LPS‐melatonin groups, but the damage rate LPS‐Melatonin group was decrease in the LPS group. It was determined that the MDA level in tissues of the LPS group was importantly increased compared to other groups. Additionally, TAA/NA ratio statistically significant differences were discovered between the groups. This study supports the potential protective effects of 10 mg/kg melatonin by modulating critical markers of local immune reaction in a model of LPS‐induced sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Detection of melatonin protective effects in sepsis via argyrophilic nucleolar regulatory region‐associated protein synthesis and TLR4/NF‐κB signaling pathway

et al. 2022

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Exploring the Chemical Constituents and Nutritive Potential of Bee Drone (Apilarnil): Emphasis on Antioxidant Properties

Abd El‐Wahed,

Khalifa,

Aldahmash

et al. 2024

Chemistry & Biodiversity

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A lesser‐known bee product called drone brood homogenate (DBH, apilarnil) has recently attracted scientific interest for its chemical and biological properties. It contains pharmacologically active compounds that may have neuroprotective, antioxidant, fertility‐enhancing, and antiviral effects. Unlike other bee products, the chemical composition of bee drone larva is poorly studied. This study analyzed the chemical compostion of apilarnil using several methods. These included liquid chromatography‐mass spectrometry (LC‐MS/MS) and a combination of gas chromatography/mass spectrometry with solid phase micro‐extraction (SPME‐GC‐MS). Additionally, antioxidant activity of the apilarnil was assessed using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay. A chemical assessment of apilarnil showed that it has 6.3± 0.00, 74.67 ± 0.10%, 3.65±0.32%, 8.80±1.01%, 13.16±0.94%, and 8.79±0.49 % of pH, moisture, total lipids, proteins, flavonoids, and carbohydrates, respectively. LC‐MS/MS analysis and molecular networking (GNPS) of apilarnil exhibited 44 compounds, including fatty acids, flavonoids, glycerophospholipids, alcohols, sugars, amino acids, and steroids. GC‐MS detected 30 volatile compounds in apilarnil, mainly esters (24%), ketones (23.84%), ethers (15.05%), alcohols (11.41%), fatty acids (10.06), aldehydes (6.73%), amines (5.46), and alkene (5.53%). The antioxidant activity of apilarnil was measured using DPPH with an IC50 of 179.93±2.46 µg/ml.

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Evaluation of the protective role of resveratrol against sepsis caused by LPS via TLR4/NF‐κB/TNF‐α signaling pathways: Experimental study

Okan

Doğanyiğit

Yılmaz

et al. 2023

Cell Biochemistry & Function

Self Cite

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The development and progression of sepsis are multifactorial and influence the immunological, endocrine, and cardiovascular systems of the body. Our knowledge of the key mechanisms involved in the pathogenesis of sepsis has expanded exponentially, yet this still needs to be translated into effective targeted therapeutic regimes. In the present study, we aimed to determine whether resveratrol has positive effects in the experimental sepsis rat model. Twenty‐eight male Spraque–Dawley rats were randomly divided into four groups (n = 7) as follows: control, lipopolysaccharide (LPS) (30 mg/kg dose), resveratrol, and LPS and resveratrol. After the experiment, liver and kidney tissues were collected for histopathological evaluation, blood serums were collected to measure malondialdehyde levels with enyzme‐linked immunosorbent assay, and Toll‐like receptor‐4 (TLR4), tumor necrosis factor‐α (TNF‐α), nuclear factor‐κB (NF‐κB) immunoreactivity density was evaluated immunohistochemically. In addition, messenger RNA expression levels for TLR4, TNF‐α, NF‐κB, interleukin‐1β, and interleukin 6 were measured. In addition, the damage observed in liver and kidney tissue was determined by AgNOR (argyrophilic nucleolar organizer regions) staining. LPS application caused severe tissue damage, oxidative stress, and increased the expressions of proinflammatory proteins and genes we evaluated, while resveratrol application eliminated these negativities. Resveratrol has been proven to suppress the TLR4/NF‐κB/TNF‐α pathway, a possible therapeutic signaling pathway that is important in initiating the inflammatory response in an animal model of sepsis.

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Nephroprotective effect of apilarnil in lipopolysaccharide-induced sepsis through TLR4/NF-κB signaling pathway

Cited by 17 publications

References 44 publications

Detection of melatonin protective effects in sepsis via argyrophilic nucleolar regulatory region‐associated protein synthesis and TLR4/NF‐κB signaling pathway

Detection of melatonin protective effects in sepsis via argyrophilic nucleolar regulatory region‐associated protein synthesis and TLR4/NF‐κB signaling pathway

Exploring the Chemical Constituents and Nutritive Potential of Bee Drone (Apilarnil): Emphasis on Antioxidant Properties

Evaluation of the protective role of resveratrol against sepsis caused by LPS via TLR4/NF‐κB/TNF‐α signaling pathways: Experimental study

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