Nephroprotective effect of ethanolic extract of Azima tetracantha root in glycerol induced acute renal failure in Wistar albino rats

Abstract

The gravity of the impact of renal failure on human health is well known and as there is no specific pharmacotherapy for renal failure, the current study was undertaken to evaluate the effect of root extract of Azima tetracantha, an ancient medicinal plant used in Siddha and Ayurvedhic systems of medicine.The experiment was done in glycerol-induced acute renal failure in Wistar albino rats. Thirty rats were divided into five groups. Group 1 was given normal saline (10 ml/kg) per oral, group 2 with single dose of hypertonic glycerol (8 ml/kg) by intramuscular injection into the hind limbs, group 3 with glycerol and ethanolic extract of A. tetracantha root (ATR) 250 mg/kg, group 4, glycerol and ATR 500 mg/kg and group 5, 500 mg/kg ATR. Extract was given orally 60 min prior to glycerol injection. 24 h urine output, serum creatinine, blood urea nitrogen, total proteins and albumin were measured for all the groups. Kidneys were examined for histopathological changes.The antioxidant activity of the extract was tested in vitro and in vivo. Rats treated with ATR showed significant improvement in biochemical parameters and histopathological changes compared to glycerol treated group. The protective effect was highly significant at 500 mg/kg. Both in vitro and in vivo assays showed significant antioxidant activity. The in vitro activity was comparable to vitamin-C.The ethanolic extract of ATR has nephroprotective effect in glycerol-induced acute renal failure and the mechanism of action could be the antioxidant effect.

Evaluation of Antiepileptic Activity of Ethanolic Extract of Azima Tetracantha Root in Mice

“…Most of the plant products evaluated for antiepileptic activity are reported to have antioxidant activity due to their phytochemical content. The in vitro antioxidant activity of Azima tetracantha root extract and in vivo activity of kidney tissue has been reported by konda et al 2015 [19]. Hence it can be inferred that the antiepileptic activity of EEATR could be not only due to its effect GABAergic transmission and calcium channel blockade but also could be due to antioxidant activity.…”

“…Pharmacological actions reported for AT are anti-inflammatory, analgesic, antipyretic, antimicrobial, antifungal, antiulcer, diuretic, anticancer, anti-snake venom, and hepatoprotective and antioxidant activities [16][17][18]. The nephroprotective effect in acute renal failure has been recently reported [19].…”

Nephroprotective effect of Berberis baluchistanica against gentamicin-induced nephrotoxicity in rabbit

“…These observations are correlated well with the renal histological findings, which explained extensive and marked tubular necrosis. The nephroprotection by medicinal plants may be by inhibiting the production of ROS and lipid peroxidation (Fahmi et al, 2016;Konda et al, 2016;Tilili et al, 2017).…”

“…More interest has developed to the protective effects of medicinal plants against chemically induced toxicities (Frei and Higdon, 2003). For example, Azima tetracantha (Konda et al, 2016) and Capparis spinosa (Tlili et al, 2017), Cestrum nocturnum (Saleem et al, 2017), Morus alba (Ullah et al, 2016), Nigella sativa (Begum et al, 2006), Sechium edule (Mumtaz et al, 2012), Terminalia muelleri (Fahmy et al, 2016) show nephroprotective effect.…”

Nephroprotective effect of ethanolic extract of abutilon indicum root in gentamicin induced acute renal failure

“…There are many plants have been screened for nephro protective activity both in acute and chronic renal failure models in India. 10,11 Abutilon indicum is belongs to malvaceae family. It has been used for commonly called 'Country mallow' is a perennial plant up to 3 m in height.…”