Nephroprotective Effects of Benzyl Isothiocyanate and Resveratrol Against Cisplatin-Induced Oxidative Stress and Inflammation

Abstract

This study was performed to compare the nephroprotective effects of benzyl isothiocyanate (BITC) and resveratrol (RES) and investigate the nephroprotective efficacy of their combination against cisplatin-induced acute renal injury. Five animal groups (each of eight) received either normal saline, a single intraperitoneal injection of cisplatin (20 mg/kg) at the sixth day, cisplatin plus oral RES (30 mg/kg) or BITC (100 mg/kg in diet), or a combination of both for 10 days. Compared to saline-treated mice, cisplatin-intoxicated mice had significantly higher (p < 0.05) serum levels of urea, creatinine, interleukin-1β (IL-1β), and tumor necrosis factor-α. Moreover, biochemical analysis of kidney tissue homogenates showed that cisplatin intoxication was associated with significantly higher (p < 0.05) tissue levels of malondialdehyde (MDA) and lower levels of reduced glutathione and activities of endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in comparison to normal controls. Histopathological and immunohistochemical examinations of renal tissue slices from cisplatin-intoxicated mice showed interstitial leukocytic infiltration, tortuous tubules with vacuolated epithelium, luminal casts, and overexpression of cyclooxygenase-II enzyme. On the other hand, treatment with RES or BITC ameliorated all the previous parameters. The effects of both compounds were comparable in all assessed parameters, except IL-1β serum concentration and renal tissue MDA concentration (which were significantly lower in the RES group). Interestingly, treatment with BITC and RES combination restored the normal concentrations of all the aforementioned biochemical parameters, as well as near normal histological and immunohistochemical pictures. In conclusion, BITC exerted nearly comparable nephroprotective, antioxidant, and anti-inflammatory effects to RES and the combination of both agents showed more potent nephroprotective effects against cisplatin than each one alone.

Protective role of luteolin against bisphenol A‐induced renal toxicity through suppressing oxidative stress, inflammation, and upregulating Nrf2/ARE/ HO‐1 pathway

“…The cell membranes consist of lipids as its main component, and thus LPO can lead to cell damage and apoptosis. The peroxidation stage leads to the formation of MDA and the presence of MD in the cells confirms radically induced LPO . The present study stated an increase in MDA concentration in the renal cells in the case of BPA‐induced rats, thus confirming the condition of OS.…”

“…Nrf2 then translocates to the nucleus and binds to the ARE . This binding causes the downstream activation of HO‐1 . The present study stated enhanced nuclear accumulation of Nrf2 by triptolide in case of the reperfused kidney.…”

The effects of vitamins and selenium mixture against brain tissue induced byd‐galactosamine

“…In general, antioxidants stabilize cell membranes (particularly, neuronal membranes) by counteracting lipid peroxidation . Antioxidant substances are plant extracts such as chard, spirulina platensis, resveratrol, cinnamon, grape seed, melatonin, quercetin, and others. All these antioxidants have proved their beneficial protective role against tissues, lipid peroxidation, and oxidative stress.…”

Molecular Mechanisms of the Anti-Cancer Effects of Isothiocyanates from Cruciferous Vegetables in Bladder Cancer

“…Similar to other ITC members, BITC has immunomodulatory, anti-microbial, and anti-oxidative activities under various pathological conditions [34,65,66] Several studies have also shown that BITC possesses anti-cancer and chemopreventive effects in various types of malignancies [67][68][69]. However, there is limited information on its anti-cancer effects and molecular mechanisms in UC.…”