Nephroprotective effects of (−)-α-bisabolol against ischemic-reperfusion acute kidney injury

Sampaio, Tiago Lima; Menezes, Ramon Róseo Paula Pessoa Bezerra de; Costa, Marcus Felipe Bezerra da; Meneses, Gdayllon Cavalcante; Arrieta, Mauren Cristina Villalta; Filho, Adriano José Maia Chaves; Morais, Glayciane Bezerra de; Libório, Alexandre Braga; Alves, Renata; Evangelista, Janaína Serra Azul Monteiro; Martins, Alice Maria Costa

doi:10.1016/j.phymed.2016.11.008

Cited by 33 publications

(25 citation statements)

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“…In this study, we observed the increase in MDA levels ( Fig. 2E) related to the AKI onset, as shown previously by Da Costa et al 2015, Sampaio et al (2016) and Kadkhodaee et al (2014). MDA and GSH levels were measured in renal tissue to indirectly assess oxidative stress.…”

Section: Resultssupporting

confidence: 87%

Evaluation of KIM-1 as an early biomarker of snakebite-induced AKI in mice

Dantas

Sampaio

Lima

et al. 2018

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Acute kidney injury (AKI) is one of the most important complications of bothropic poisoning and its early identification remains challenging. The nephrotoxicity of Bothrops insularis venom (BinsV) was previously described by our research group. In this study, we continued to evaluate the effect of BinsV on kidney function in mice and LLC-MK2 proximal tubule cells, evaluating KIM-1 protein as an early AKI biomarker. Male Swiss mice were inoculated with BinsV intramuscularly and observed for 24 h in a metabolic cage model. Urine and blood were collected for biochemical analyses and the kidneys were examined for oxide-reducing balance and submitted to histological analysis. LLC-MK2 cells incubated with BinsV were assessed for cell viability and cell death mechanism by flow cytometry. Histological analysis of the kidneys indicated AKI and the oxide-reducing analyses demonstrated a decreasing in reduced glutathione (GSH) levels and an increasing on Malondialdehyde (MDA) levels. BinsV was cytotoxic to LLC-MK2 and the cytometry analyses suggested necrosis. Within 24 h after the envenomation, urinary creatinine did not increase, but the urinary levels of KIM-1 increased. In conclusion, we found AKI evidence in the kidney tissue and the increase in the KIM-1 levels suggest it can be used as an early AKI biomarker.

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Section: Resultssupporting

confidence: 87%

Evaluation of KIM-1 as an early biomarker of snakebite-induced AKI in mice

Dantas

Sampaio

Lima

et al. 2018

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“…It showed the ability to lessen oxidative stress and delay the development of various disease complications by countering ROS production as in isoproterenol-induced myocardial infarction [68]. Moreover, (−)-α-bisabolol has exhibited in vivo and in vitro nephroprotective activity in kidney ischemic-reperfusion models in vivo, through the attenuation of functional alterations and reduction of lipid peroxidation and urinary kidney injury molecule-1 (uKIM-1) levels, which was associated with an improvement of GSH levels in male Wistar rats, and in vitro through enhancing cell viability and decreasing ROS production in Rhesus Monkey Kidney Epithelial Cells (LLC-MK2 cells) [69] and inhibiting NADPH oxidase enzyme activity in HK2 cells [70]. In addition, cinnamaldehyde inhibits ROS production in association with the up-regulation of the antioxidative enzyme heme-oxygenase 1 (HO-1).…”

Section: Discussionmentioning

confidence: 99%

Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

et al. 2020

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This study aimed to describe the protective efficacy of Moringa oleifera ethanolic extract (MOEE) against the impact of cobalt chloride (CoCl2) exposure on the rat’s kidney. Fifty male rats were assigned to five equal groups: a control group, a MOEE-administered group (400 mg/kg body weight (bw), daily via gastric tube), a CoCl2-intoxicated group (300 mg/L, daily in drinking water), a protective group, and a therapeutic co-administered group that received MOEE prior to or following and concurrently with CoCl2, respectively. The antioxidant status indices (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)), oxidative stress markers (hydrogen peroxide (H2O2), 8-hydroxy-2-deoxyguanosine (8-OHdG), and malondialdehyde (MDA)), and inflammatory response markers (nitric oxide (NO), tumor necrosis factor (TNF-α), myeloperoxidase (MPO), and C-reactive protein (CRP)) were evaluated. The expression profiles of pro-inflammatory cytokines (nuclear factor-kappa B (NF-kB) and interleukin-6 (IL-6)) were also measured by real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that CoCl2 exposure was associated with significant elevations of oxidative stress and inflammatory indices with reductions in the endogenous tissue antioxidants’ concentrations. Moreover, CoCl2 enhanced the activity of the NF-κB inflammatory-signaling pathway that plays a role in the associated inflammation of the kidney. MOEE ameliorated CoCl2-induced renal oxidative damage and inflammatory injury with the suppression of the mRNA expression pattern of pro-inflammatory cytokine-encoding genes. MOEE is more effective when it is administered with CoCl2 exposure as a prophylactic regimen. In conclusion, MOEE administration exhibited protective effects in counteracting CoCl2-induced renal injury in rats.

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“…Samples were incubated in the dark for 30 min, washed twice with PBS, and re-suspended in PBS (500 µL/tube). Finally, the samples were analyzed using flow cytometry [73]. Results were evaluated using relative fluorescent intensity, calculated as described in the previous item.…”

Section: Methodsmentioning

confidence: 99%

Trypanocidal Mechanism of Action and in silico Studies of p-Coumaric Acid Derivatives

Lopes

Castillo

Monteiro

et al. 2019

IJMS

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Trypanosoma species are responsible for chronic and systemic infections in millions of people around the world, compromising life quality, and family and government budgets. This group of diseases is classified as neglected and causes thousands of deaths each year. In the present study, the trypanocidal effect of a set of 12 ester derivatives of the p-coumaric acid was tested. Of the test derivatives, pentyl p-coumarate (7) (5.16 ± 1.28 μM; 61.63 ± 28.59 μM) presented the best respective trypanocidal activities against both epimastigote and trypomastigote forms. Flow cytometry analysis revealed an increase in the percentage of 7-AAD labeled cells, an increase in reactive oxygen species, and a loss of mitochondrial membrane potential; indicating cell death by necrosis. This mechanism was confirmed by scanning electron microscopy, noting the loss of cellular integrity. Molecular docking data indicated that of the chemical compounds tested, compound 7 potentially acts through two mechanisms of action, whether by links with aldo-keto reductases (AKR) or by comprising cruzain (CZ) which is one of the key Trypanosoma cruzi development enzymes. The results indicate that for both enzymes, van der Waals interactions between ligand and receptors favor binding and hydrophobic interactions with the phenolic and aliphatic parts of the ligand. The study demonstrates that p-coumarate derivatives are promising molecules for developing new prototypes with antiprotozoal activity.

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Nephroprotective effects of (−)-α-bisabolol against ischemic-reperfusion acute kidney injury

Abstract: (-)-α-Bisabolol has a nephroprotective effect in kidney I/R, with antioxidant effect. Moreover, this result seems to be associated to a direct protective effect on tubular epithelia.

Cited by 33 publications

References 45 publications

Evaluation of KIM-1 as an early biomarker of snakebite-induced AKI in mice

Evaluation of KIM-1 as an early biomarker of snakebite-induced AKI in mice

Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

Trypanocidal Mechanism of Action and in silico Studies of p-Coumaric Acid Derivatives

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