Nerve fibroblast impact on Schwann cell behavior

Dreesmann, Lars; Mittnacht, Ursula; Lietz, Martin; Schlosshauer, Bürkhard

doi:10.1016/j.ejcb.2009.01.001

Cited by 57 publications

(44 citation statements)

References 46 publications

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“…Monolayers were dissolved in lysis buffer, and equal protein concentrations were subjected to SDS-PAGE (10% gels) [29]. Protein concentrations were quantified by using the BCA Protein Assay Kit (Pierce, Rockford, IL, USA).…”

Section: Western Blottingmentioning

confidence: 99%

Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

Baumer

Funk

Schlosshauer

2010

Cell. Mol. Life Sci.

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By counteracting the shortening of chromosome telomeres, telomerase reverse transcriptase (hTERT) prevents senescence and age-related cell death. Embryonic cells display a high telomerase activity that declines rapidly with cell differentiation. Conversely, de-differentiated tumor cells tend to re-express telomerase. In view of the controversial data on the reciprocal correlation between cell proliferation and differentiation, we questioned whether telomerase overexpression and the resulting immortalization would affect the functional phenotype of human endothelial cells. Our comparative analysis addressed (1) distinct cell adhesion to different ECM-proteins analyzed on miniaturized multisubstrate arrays (MSA), (2) protein expression of diverse markers, (3) the uptake of DiI-Ac-LDL, (4) the inflammatory response based on upregulation of ICAM-1, (5) tube formation, and (6) the barrier properties of cell monolayers in transfilter cultures. Our results, based on some 40 data sets, demonstrate that immortalization of primary endothelial cells by hTERT maintains the typical endothelial characteristics without any sign of functional de-differentiation.

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Section: Western Blottingmentioning

confidence: 99%

Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

Baumer

Funk

Schlosshauer

2010

Cell. Mol. Life Sci.

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“…However, fibroblasts (FIBs) in the connective tissue surrounding the axoneSchwann cell bundles usually have stronger mobility [5]. After injury, they migrate into the wound sites in the first place and secrete collagen and transforming growth factors (TGFs) which encourage the formation of scar tissue, impeding the migration of Schwann cells and thus regeneration of functional nerve tissue [6,7]. Therefore, it is of great importance to develop a material which is able to specifically guide the directional migration of Schwann cells, whereas suppresses the migration of fibroblasts.…”

Section: Introductionmentioning

confidence: 99%

A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells

Ren

Mao

et al. 2015

Biomaterials

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“…1 However, an excessive fibroblastic response with scar tissue replacing normal tissue may serve to inhibit nerve regeneration by creating a nonpermissive environment by inhibiting Schwann cell proliferation which is essential in nerve regeneration. [2][3][4] In addition, interfascicular connective tissue, while essential for supporting normal tissue, is observed in excess in chronic compression disorders and after surgical repair, theoretically compromising nerve regeneration. 5 While excessive intraneural connective tissue formation or scarring may compromise nerve regeneration, perineural scarring may cause pain by tethering nerves to the surrounding soft tissue.…”

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confidence: 99%

Collagen nerve protector in rat sciatic nerve repair: A morphometric and histological analysis

et al. 2010

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Peripheral nerve repair is often complicated by fibroblastic scar formation, nerve dysfunction, and traumatic neuroma formation. Use of bio-absorbable protective wraps may improve outcomes of these repairs. This study histologically compared the incidence of neuroma formation, connective tissue proliferation, and axonal regrowth in transected rat sciatic nerves repaired with and without tubular collagen nerve sleeves. Twenty Sprague-Dawley rats underwent unilateral sharp sciatic nerve transection and microscopic nerve repair with four epineural sutures and were randomly treated with or without an encircling collagen nerve sleeve. Normal nerves from the contralateral sciatic nerve were also examined. At sacrifice three months later, the nerves were evaluated for traumatic neuroma formation, perineural scar formation, and morphometric analysis. Histological examination of normal and repaired nerves by a neuropathologist demonstrated healing, minimal Wallerian degeneration and no traumatic neuroma formation. Distal section analysis (nine nonwrapped, 10 wrapped), revealed no significant differences in total fascicular area, myelinated fibers per nerve, fiber density, myelin area per nerve, myelinated fiber diameter, axon diameter, myelin thickness, or G-ratio. Significantly greater (P = 0.005) inner epineural connective tissue formation was observed in nonwrapped nerves (0.62 mm(2) +/- 0.2) versus wrapped nerves (0.35 mm(2) +/- 0.16). The ratio of connective tissue to fascicular area was larger in nonwrapped (1.08 +/- 0.26) versus wrapped nerves (0.63 +/- 0.22) (P < 0.001). This study demonstrated decreased inner epineural connective tissue formation with use of a collagen nerve wrap during primary repair of peripheral nerve transection in a rat sciatic nerve model.

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Nerve fibroblast impact on Schwann cell behavior

Cited by 57 publications

References 46 publications

Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

Does telomerase reverse transcriptase induce functional de-differentiation of human endothelial cells?

A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells

Collagen nerve protector in rat sciatic nerve repair: A morphometric and histological analysis

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