Nerve growth factor from Indian Russell’s viper venom (RVV-NGFa) shows high affinity binding to TrkA receptor expressed in breast cancer cells: Application of fluorescence labeled RVV-NGFa in the clinical diagnosis of breast cancer

Islam, Taufikul; Majumder, Munmi; Bidkar, Anil P.; Ghosh, Siddhartha Sankar; Mukhopadhyay, Rupak; Utkin, Yuri N.; Mukherjee, Ashis K.

doi:10.1016/j.biochi.2020.06.004

Cited by 10 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Noteworthy, several studies have also shown the overexpression of TrkA and NGFR/p75NTR receptors in most of the human breast cancers as compared to the expression of these receptors in normal cells [94,114]. Moreover, Islam et al [14] demonstrated that Russell's Viper Venom (RVV)-NGFa (an NGF isoform), labelled with Fluorescein Isothiocyanate (FITC), establishes strong binding to TrkA and NGFR/p75NTR receptors in breast cancer cells but not in non-cancerous cells. This is a promising result for the future development of a tool using a fluorescent molecule-tagged RVV-NGFa binding technique to differentiate cancerous from non-cancerous cells and thus to diagnose breast cancer [14].…”

Section: Ngf and Its Receptors As Diagnostic Markers For Breast Cancermentioning

confidence: 99%

“…Since the 1990s, several studies have indicated that NGF and its receptors could also play a key role in the pathogenesis of breast cancer and consequently could represent a new therapeutic target [9][10][11][12][13]. Other evidence indicates that both NGF and its receptors could be considered as accurate diagnostic and prognostic tools for breast cancer [14][15][16][17]. Moreover, the NGF precursor (pro-NGF) signaling pathways were related to breast cancer invasion and metastasis [18,19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression and Signaling Pathways of Nerve Growth Factor (NGF) And Pro-NGF in Breast Cancer: A Systematic Review

Bruno¹,

Arcuri²,

Vozzo³

et al. 2022

Preprint

View full text Add to dashboard Cite

Breast cancer represents the most frequent cancer and the leading cause of cancer death among women. Thus, the prevention and early diagnosis of breast cancer appears to be of primary urgency as well as the development of new treatments able to improve its prognosis. Nerve Growth Factor (NGF) is a neurotrophic factor that plays a key role in the regulation of neuronal functions thought the binding to the Tropomyosin receptor kinase A (TrkA) and the Nerve Growth Factor receptor or Pan-Neurotrophin Receptor 75 (NGFR/p75NTR). Also, its precursor (pro-NGF) can extert biological activity by forming a trimeric complex with NGFR/p75NTR and sortilin or by binding to TrkA receptors with low affinity. Both in vitro and in vivo studies showed that NGF is synthesized and released by breast cancer cells and has mitogen, antiapoptotic and angiogenic effects on these cells through the activation of different signaling cascades that involve TrkA and NGFR/p75NTR receptors. Conversely, pro-NGF signaling has been related to breast cancer invasion and metastasis. Other studies suggested that NGF and its receptors could represent a good diagnostic and prognostic tool, as well as promising therapeutic targets for breast cancer. Here, we comprehensively summarize and systematically review the current experimental evidence on this topic.

show abstract

Section: Ngf and Its Receptors As Diagnostic Markers For Breast Cancermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression and Signaling Pathways of Nerve Growth Factor (NGF) And Pro-NGF in Breast Cancer: A Systematic Review

Bruno¹,

Arcuri²,

Vozzo³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In the current issue, Islam and coworkers, provide evidence that a low molecular weight protein with the ability to induce neurite outgrowth in the pheochromocytoma cell line model (PC‐12 cells) (Islam et al., 2020). Blockade experiments using anti‐NGF antibodies, demonstrate that the neuritogenesis properties of the isolated protein are not related to NGF, but rather to a long chain α‐neurtoxin named Nn‐α‐elapitoxin.…”

Section: Neuritogenesis Properties Of Nn‐α‐elapitoxinmentioning

confidence: 99%

A unique snake venom neuritogenesis mechanism: A cornerstone in the treatment of neurodegenerative diseases?

Hernández‐Kelly¹,

Ortega²

2020

Journal of Neurochemistry

View full text Add to dashboard Cite

Neurodegenerative diseases are a worldwide health problem and are a major cause of death and disability. A progressive loss of defined neuronal populations is triggered by a diverse array of stimuli that converge in deficient neurotrophic signaling. Therefore, much effort has been placed in recent years in the characterization of the molecular mechanisms associated with the structure and function of neurotrophins, its receptors, signaling strategies, and their target genes. This Editorial highlights an impressive study by the group of Prof. Ashis K. Mukherjee, a renowned specialist in snake venoms, in which a component of the Indian Cobra N.naja venom with no significant similarity to nerve growth factor, is shown to induce sustained neuritogenesis. An elegant transcriptomic and functional analysis of this component, named Nn-α-elapitoxin, mapped novel domains in mammalian neurotrophic receptors that trigger both conventional and novel signal cascades that support neurite extension in the PC-12 neuronal model system. The authors discuss their findings in the context of the paradoxical neurite outgrowth properties of this toxin which originate in their unique receptor binding site. This study takes an important step towards a better understanding of the complexity of neuronal development and maintenance of the nervous system and provides a potential target to improve neurotrophic signaling, independent of endogenous growth factors, in the diseased brain.

show abstract

“…Since the 1990s, several studies have indicated that NGF and its receptors could also play a key role in the pathogenesis of breast cancer and, consequently, could represent a new therapeutic target [ 9 , 10 , 11 , 12 , 13 ]. Other evidence indicates that both NGF and its receptors could be considered accurate diagnostic and prognostic tools for breast cancer [ 14 , 15 , 16 , 17 ]. Moreover, the NGF precursor (pro-NGF) signaling pathways were related to breast cancer invasion and metastasis [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Expression and Signaling Pathways of Nerve Growth Factor (NGF) and Pro-NGF in Breast Cancer: A Systematic Review

et al. 2022

View full text Add to dashboard Cite

Breast cancer represents the most common type of cancer and is the leading cause of death due to cancer among women. Thus, the prevention and early diagnosis of breast cancer is of primary urgency, as well as the development of new treatments able to improve its prognosis. Nerve Growth Factor (NGF) is a neurotrophic factor involved in the regulation of neuronal functions through the binding of the Tropomyosin receptor kinase A (TrkA) and the Nerve Growth Factor receptor or Pan-Neurotrophin Receptor 75 (NGFR/p75NTR). In addition, its precursor (pro-NGF) can extert biological activity by forming a trimeric complex with NGFR/p75NTR and sortilin, or by binding to TrkA receptors with low affinity. Several examples of in vitro and in vivo evidence show that NGF is both synthesized and released by breast cancer cells, and has mitogen, antiapoptotic and angiogenic effects on these cells through the activation of different signaling cascades that involve TrkA and NGFR/p75NTR receptors. Conversely, pro-NGF signaling has been related to breast cancer invasion and metastasis. Other studies suggested that NGF and its receptors could represent a good diagnostic and prognostic tool, as well as promising therapeutic targets for breast cancer. In this paper, we comprehensively summarize and systematically review the current experimental evidence on this topic. INPLASY ID: INPLASY2022100017.

show abstract

Nerve growth factor from Indian Russell’s viper venom (RVV-NGFa) shows high affinity binding to TrkA receptor expressed in breast cancer cells: Application of fluorescence labeled RVV-NGFa in the clinical diagnosis of breast cancer

Cited by 10 publications

References 39 publications

Expression and Signaling Pathways of Nerve Growth Factor (NGF) And Pro-NGF in Breast Cancer: A Systematic Review

Expression and Signaling Pathways of Nerve Growth Factor (NGF) And Pro-NGF in Breast Cancer: A Systematic Review

A unique snake venom neuritogenesis mechanism: A cornerstone in the treatment of neurodegenerative diseases?

Expression and Signaling Pathways of Nerve Growth Factor (NGF) and Pro-NGF in Breast Cancer: A Systematic Review

Contact Info

Product

Resources

About